FB2026_02 , released June 18, 2026
Allele: Dmel\RxKO
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General Information
Symbol
Dmel\RxKO
Species
D. melanogaster
Name
FlyBase ID
FBal0370821
Feature type
allele
Associated gene
Dmel\Rx
Associated Insertion(s)
TI{mCherry}RxKO
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Klöppel et al., 2021)
gene targeting by homologous recombination
(Klöppel et al., 2021)
Progenitor genotype
Associated Insertion(s)
TI{mCherry}RxKO
Cytology
Description

394bp of the endogenous Rx locus has been deleted; this removes the ATG codon in the first exon and sequence encoding the first 123 amino acids of Rx. The deleted sequence has been replaced by a cassette that comprises a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal, 2: a w+* marker. The mCherry coding region is expected to be expressed under the control of the endogenous Rx regulatory sequences.

(Klöppel et al., 2021)
Allele components
Component
Use(s)
Inserted element
TI{mCherry}
Encoded product / tool
mCherry
Also carries
attP
loxP
mFRT71
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DRxKO
      (Klöppel et al., 2021)
      RxKO
      Name Synonyms
      Secondary FlyBase IDs
        References (1)