UASp regulatory sequences drive expression of mutant egl (contains K694A, R695A and R699A substitutions), tagged at the C-terminal with 3xTag:FLAG. Contains silent mutations so that the construct is RNAi resistant (not targeted by the shRNA produced by eglGL01170).
Scer\GAL4VP16.nanos.UTR/eglRBD6.UASp.RR.FLAG fails to rescue eglGL01170