FlyBase Allele Report: Hsap\HIPK1[UAS.Tag:MYC]

General Information

Symbol

Hsap\HIPK1^UAS.Tag:MYC

Species

H. sapiens

Name

FlyBase ID

FBal0371180

Feature type

allele

Associated gene

Hsap\HIPK1

Associated Insertion(s)

Carried in Construct

P{UAS-hHIPK1.MYC}

Key Links

Transgenic product class

cDNA

(Kinsey et al., 2021)

wild_type

(Kinsey et al., 2021)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kinsey et al., 2021)

wild_type

(Kinsey et al., 2021)

Mutagen

in vitro construct

(Kinsey et al., 2021)

Progenitor genotype

Carried in construct

P{UAS-hHIPK1.MYC}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\HIPK1 isoform 1 cDNA. Tagged at the N-terminal with Tag:MYC.

(Kinsey et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\HIPK1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | larval stage, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

neoplasia | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Kinsey et al., 2021)

visible | adult stage, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

Phenotype Manifest In

arista | absent, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

chaeta | ectopic, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

leg, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

mesothoracic leg, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

metathoracic leg, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

sex comb | ectopic, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

wing, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

wing disc, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

wing disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Kinsey et al., 2021)

wing hair | ectopic, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

wing vein, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

Detailed Description

Statement

Reference

The expression of Hsap\HIPK1^UAS.Tag:MYC under the control of Scer\GAL4^dpp.blk1 leads to increased apoptosis in the wing disc. Wings show severe notches, vein abnormalities, altered pigmentation, as well as ectopic small hairs and sensory bristles resembling those found on halteres. Legs show mild malformations, including ectopic sex combs on the middle and rear legs of males, where they are not normally found.

Wing disc clonal expression of Hsap\HIPK1^UAS.Tag:MYC under the control of Scer\GAL4^Act5C.PI leads to increased eye disc size, which is becomes highly distorted.

(Kinsey et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Hipk-BG00855/Hsap\HIPK1^UAS.Tag:MYC is a suppressor | partially of increased mortality during development phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

NOT Suppressor of