FlyBase Allele Report: Hsap\HIPK3[UAS.Tag:MYC]

General Information

Symbol

Hsap\HIPK3^UAS.Tag:MYC

Species

H. sapiens

Name

FlyBase ID

FBal0371182

Feature type

allele

Associated gene

Hsap\HIPK3

Associated Insertion(s)

Carried in Construct

P{UAS-hHIPK3.MYC}

Key Links

Transgenic product class

cDNA

(Kinsey et al., 2021)

wild_type

(Kinsey et al., 2021)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kinsey et al., 2021)

wild_type

(Kinsey et al., 2021)

Mutagen

in vitro construct

(Kinsey et al., 2021)

Progenitor genotype

Carried in construct

P{UAS-hHIPK3.MYC}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\HIPK3 isoform 2 cDNA. Tagged at the N-terminal with Tag:MYC.

(Kinsey et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\HIPK3

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | larval stage, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

increased size | larval stage, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

neoplasia | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Kinsey et al., 2021)

visible | adult stage, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

Phenotype Manifest In

leg, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

mesothoracic leg, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

metathoracic leg, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

sex comb | ectopic, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

wing disc, with Scer\GAL4^dpp.blk1

(Kinsey et al., 2021)

wing disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Kinsey et al., 2021)

Detailed Description

Statement

Reference

The expression of Hsap\HIPK3^UAS.Tag:MYC under the control of Scer\GAL4^dpp.blk1 leads to increased mitosis in the wing disc, as well as enlargement of the dpp expression domain. Legs show severe malformations, including ectopic sex combs on the middle and rear legs of males, where they are not normally found.

Wing disc clonal expression of Hsap\HIPK2^UAS.Tag:MYC under the control of Scer\GAL4^Act5C.PI leads to increased eye disc size, which is becomes highly distorted.

(Kinsey et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^Hipk-BG00855/Hsap\HIPK3^UAS.Tag:MYC is a non-suppressor of lethal - all die before end of P-stage phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

Scer\GAL4^Hipk-BG00855/Hsap\HIPK3^UAS.Tag:MYC is a non-suppressor of decreased size | pharate adult stage phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Hipk-BG00855/Hsap\HIPK3^UAS.Tag:MYC is a suppressor of ocellus | pharate adult stage | absent phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Hipk-BG00855/Hsap\HIPK3^UAS.Tag:MYC is a non-suppressor of eye | pharate adult stage phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

Scer\GAL4^Hipk-BG00855/Hsap\HIPK3^UAS.Tag:MYC is a non-suppressor of ocellar bristle | pharate adult stage | decreased number phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

Scer\GAL4^Hipk-BG00855/Hsap\HIPK3^UAS.Tag:MYC is a non-suppressor of vertical bristle 2 | pharate adult stage | decreased number phenotype of Hipk^BG00855/Hipk⁴

(Kinsey et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Hsap\HIPK3^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close