FB2026_02 , released June 18, 2026
Allele: Dmel\PolG2HomeR
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General Information
Symbol
Dmel\PolG2HomeR
Species
D. melanogaster
Name
FlyBase ID
FBal0371725
Feature type
allele
Associated gene
Dmel\PolG2
Associated Insertion(s)
TI{TI}PolG2HomeR
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Kandul et al., 2021)
gene targeting by homologous recombination
(Kandul et al., 2021)
Progenitor genotype
Associated Insertion(s)
TI{TI}PolG2HomeR
Cytology
Description

A 'HomeR' ('Home and Rescue') sequence cassette composed of 1. a snRNA:U6:96Aa promoter driving expression of a gRNA that targets PolG2 (target sequence GCGCAGCCGGGACACAAGGCTGG) and 2. a Avic\GFPEGFP.3xP3.cLa marker, has been inserted into the endogenous PolG2 locus, immediately downstream of the gene. Due to the design of the donor plasmid used, 22 codons at the 3' end of the PolG2 locus (downstream from the cut site of the gRNA encoded by the inserted HomeR cassette) have been 're-coded' so that the encoded amino acids are not changed, but the sequence is resistant to the sgRNA encoded by the inserted HomeR cassette. A p10 3' UTR has been inserted downstream of the re-coded codons to support robust expression of the re-coded PolG2 gene. Used as part of a split gene-drive system.

(Kandul et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PolG2GDe
      (Gamez et al., 2021)
      PolG2HomeR
      (Kandul et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)