FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL80Tub.Tag:degron(AID)
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General Information
Symbol
Scer\GAL80Tub.Tag:degron(AID)
Species
S. cerevisiae
Name
FlyBase ID
FBal0373787
Feature type
allele
Associated gene
Scer\GAL80
Associated Insertion(s)
Carried in Construct
PBac{tubP-TIR1-2A-GAL80.AID}
Also Known As
Auxin-GAL80
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(McClure et al., 2022)
Progenitor genotype
Carried in construct
PBac{tubP-TIR1-2A-GAL80.AID}
Cytology
Description

A αTub84B promoter drives expression of a bicistronic transcript that encodes two open reading frames separated by a 2A self-cleaving peptide (to ensure separate translation of each protein). The first ORF encodes Atha\TIR1 carrying two point mutations (D170E and M473L) that improve substrate affinity and auxin sensitivity (details from PMID:26552885). The second ORF encodes GAL80 tagged with Tag:degron(AID) (minimal degron sequence has been used). In the absence of auxin, Tag:degron(AID)-tagged GAL80 is produced, which can repress GAL4 activity. In the presence of auxin, the Tag:degron(AID) degron tethers the tagged GAL80 protein to the Atha\TIR1 protein, triggering the degradation of GAL80 protein and allowing GAL4-mediated expression.

(McClure et al., 2022)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
GAL80
Tagged with
Tag:degron(AID)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL80Tub.Tag:degron(AID)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)