A chimeric protein consisting of Hsap\KMT5A amino acids 1-214 (codon optimized for Drosophila) fused to Set8 amino acids 555-691 (this protein contains the Set8 SET domain) is expressed under the control of Set8 regulatory sequences (Set8 5' and 3'UTRs are also present).
Set8::Hsap\KMT5AN-KMT5A::Set8-C/Set8::Hsap\KMT5AN-KMT5A::Set8-C is a suppressor of lethal - all die before end of pupal stage | recessive phenotype of Set820
Set8::Hsap\KMT5AN-KMT5A::Set8-C, Set820 has fertile phenotype
Set8::Hsap\KMT5AN-KMT5A::Set8-C, Set820 has visible | adult stage phenotype
Set8::Hsap\KMT5AN-KMT5A::Set8-C, Set820 has eye phenotype