FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Set8
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General Information
Symbol
Dmel\Set8
Species
D. melanogaster
Name
SET domain containing 8
Annotation Symbol
CG3307
Feature Type
protein_coding_gene
FlyBase ID
FBgn0011474
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
[histone H4]-lysine(20) N-methyltransferase (2.1.1.361)
Gene Summary
SET domain containing 8 (Set8) encodes the histone methyltransferase that controls lysine20 monomethylation of the product of His4. This histone mark is essential for normal progression through the cell cycle. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

PR-Set7, l(3)neo41, Set7, dSet8

Key Links
Genomic Location
Cytogenetic map
88B3-88B3
Sequence location
Recombination map
3-55
RefSeq locus
NT_033777 REGION:14316038..14320494
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables histone H4K20 methyltransferase activity
inferred from mutant phenotype
(Karachentsev et al., 2005, Fang et al., 2002, Nishioka et al., 2002)
enables histone H4K20 monomethyltransferase activity
inferred from mutant phenotype
(Yang et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables histone H4K20 methyltransferase activity
inferred from biological aspect of ancestor with PANTHER:PTN002920257
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR016858
(InterPro Project Members, 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in chromosome condensation
inferred from direct assay
(Sakaguchi and Steward, 2007)
inferred from genetic interaction with FLYBASE:mei-41; FB:FBgn0004367
(Sakaguchi and Steward, 2007)
involved_in DNA damage checkpoint signaling
inferred from direct assay
(Sakaguchi and Steward, 2007)
involved_in heterochromatin formation
inferred from mutant phenotype
(Karachentsev et al., 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in regulation of DNA damage response, signal transduction by p53 class mediator
inferred from biological aspect of ancestor with PANTHER:PTN002920257
(GO Reference Genome Project, 2011-)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN002920257
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in polytene chromosome
inferred from direct assay
(Karachentsev et al., 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002920257
(GO Reference Genome Project, 2011-)
is_active_in polytene chromosome
inferred from biological aspect of ancestor with PANTHER:PTN002920257
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily. (Q9VFK6)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
histone H4K20 monomethyltransferase activity
L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = N(6)-methyl-L- lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine + H(+) (2.1.1.361)
RHEA 60344:
Summaries
Gene Snapshot
SET domain containing 8 (Set8) encodes the histone methyltransferase that controls lysine20 monomethylation of the product of His4. This histone mark is essential for normal progression through the cell cycle. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
SET DOMAIN LYSINE METHYLTRANSFERASES -
SET domain lysine methyltransferases (KMTs) catalyze the addition of methyl groups to lysine residues. SET domain KMTs possess a domain that was original characterized in the D.mel KMTs Su(var)3-9, E(z) and trx. (Adapted from FBrf0216343).
Protein Function (UniProtKB)
Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, possibly by contributing to the maintenance of proper higher-order structure of DNA and chromosome condensation during mitosis.
(UniProt, Q9VFK6)
Summary (Interactive Fly)

Set domain protein - methyltransferase that targets Histone H4 - helps maintain silent chromatin by a histone modification that precludes neighboring acetylation of the H4 tail

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Set8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VFK6)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.41

Gene model reviewed during 5.53

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Set8-RA
FBtr0082929
NM_142097
3311
691
Set8-RB
FBtr0082930
NM_169577
4183
691
Set8-RD
FBtr0309996
NM_001260171
3175
691
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Set8-PA
FBpp0082388
76.5
691
7.40
Q9VFK6
NP_650354
AAF55047
Set8-PB
FBpp0082389
76.5
691
7.40
Q9VFK6
NP_731900
AAN13605
Set8-PD
FBpp0301702
76.5
691
7.40
Q9VFK6
NP_001247100
AFH06418
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

691 aa isoforms: Set8-PA, Set8-PB, Set8-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Set8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.47

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
larval lateral VNC glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic/larval midgut
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in polytene chromosome
inferred from direct assay
(Karachentsev et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Set8 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Set8
Transgenic constructs containing regulatory region of Set8
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
lethal - all die before end of larval stage (with Df(3R)red3l)
lethal - all die before end of larval stage | recessive
lethal - all die before end of pupal stage | recessive
partially lethal, with Set820
partially lethal, with Set8RG
partially lethal (with Set81)
partially lethal (with Set820)
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile, with Set820
fertile, with Set8ΔN
Other Phenotypes
Allele
abnormal body size | pupal stage | recessive
abnormal mitotic cell cycle
abnormal neuroanatomy
abnormal neuroanatomy (with Df(3R)red3l)
flightless, with Scer\GAL4Mef2.PR
melanotic mass phenotype | larval stage, with Set820
melanotic mass phenotype | larval stage, with Set8RGHL
non-enhancer of variegation, with P{hsp26-pt-T}39C-27
non-enhancer of variegation, with P{hsp26-pt-T}39C-31
non-enhancer of variegation, with P{hsp26-pt-T}39C-50
non-enhancer of variegation, with P{hsp26-pt-T}39C-55
non-enhancer of variegation, with P{hsp26-pt-T}39C-62
non-suppressor of variegation, with P{hsp26-pt-T}39C-27
non-suppressor of variegation, with P{hsp26-pt-T}39C-31
non-suppressor of variegation, with P{hsp26-pt-T}39C-50
non-suppressor of variegation, with P{hsp26-pt-T}39C-55
non-suppressor of variegation, with P{hsp26-pt-T}39C-62
some die during larval stage (with Df(3R)red3l)
some die during larval stage | recessive
some die during pupal stage | recessive
suppressor of variegation | dominant, with P{hsp26-pt-T}118E-10
suppressor of variegation | dominant, with P{hsp26-pt-T}Dyrk3118E-15
visible, with Scer\GAL4pnr-MD237
visible | adult stage, with Set820
visible | adult stage, with Set8RG
visible | adult stage, with Set8ΔN
Phenotype manifest in
Allele
anterior pupal spiracle
chromosome
condensed chromosome
embryonic/larval cuticle
embryonic/larval cuticle (with Df(3R)red3l)
eye, with Set820
eye, with Set8RG
eye, with Set8ΔN
eye (with Df(3R)red3l)
eye (with Set81)
eye (with Set820)
larval brain | third instar larval stage
larval brain | third instar larval stage (with Df(3R)red3l)
larval digestive system (with Df(3R)red3l)
larval tracheal system
leg disc
macrochaeta (with Df(3R)red3l)
melanotic mass
melanotic mass (with Df(3R)red3l)
mesonotum, with Scer\GAL4pnr-MD237
myofibril, with Scer\GAL4Mef2.PR
neuroblast | third instar larval stage
sarcomere, with Scer\GAL4Mef2.PR
wing disc
wing margin (with Df(3R)red3l)
Z disc, with Scer\GAL4Mef2.PR
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (20)
Hsap\KMT5A
7 of 14
Yes
Yes
 
1  
Hsap\ASH1L
1 of 14
No
No
Hsap\EHMT1
1 of 14
No
No
1  
Hsap\EHMT2
1 of 14
No
No
Hsap\EZH1
1 of 14
No
No
Hsap\EZH2
1 of 14
No
No
1  
Hsap\KMT2A
1 of 14
No
No
3  
Hsap\KMT2B
1 of 14
No
No
Hsap\KMT2C
1 of 14
No
No
Hsap\KMT2D
1 of 14
No
No
Hsap\NSD1
1 of 14
No
No
Hsap\NSD2
1 of 14
No
No
1  
Hsap\NSD3
1 of 14
No
No
Hsap\SETD1A
1 of 14
No
No
Hsap\SETD1B
1 of 14
No
No
Hsap\SETD2
1 of 14
No
No
Hsap\SETDB1
1 of 14
No
No
1  
Hsap\SETMAR
1 of 14
No
No
Hsap\SUV39H1
1 of 14
No
No
4  
Hsap\SUV39H2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (17)
Rnor\RGD1561318
8 of 14
Yes
Yes
Rnor\Kmt5a
7 of 14
No
Yes
Rnor\Ehmt1
1 of 14
No
No
Rnor\Ehmt2
1 of 14
No
No
Rnor\Ezh1
1 of 14
No
No
Rnor\Ezh2
1 of 14
No
No
Rnor\Kmt2a
1 of 14
No
No
Rnor\Kmt2b
1 of 14
No
No
Rnor\Nsd1
1 of 14
No
No
Rnor\Nsd2
1 of 14
No
No
Rnor\RGD1561853
1 of 14
No
Yes
Rnor\Setd1a
1 of 14
No
No
Rnor\Setd1b
1 of 14
No
No
Rnor\Setd2
1 of 14
No
No
Rnor\Setmar
1 of 14
No
No
Rnor\Suv39h1l1
1 of 14
No
No
Rnor\Suv39h2
1 of 14
No
No
Mus musculus (laboratory mouse) (16)
Mmus\Kmt5a
7 of 14
Yes
Yes
Mmus\Ehmt1
1 of 14
No
No
Mmus\Ehmt2
1 of 14
No
No
Mmus\Ezh1
1 of 14
No
No
Mmus\Ezh2
1 of 14
No
No
Mmus\Kmt2a
1 of 14
No
No
Mmus\Kmt2b
1 of 14
No
No
Mmus\Kmt2c
1 of 14
No
No
Mmus\Nsd1
1 of 14
No
No
Mmus\Nsd2
1 of 14
No
No
Mmus\Setd1a
1 of 14
No
No
Mmus\Setd1b
1 of 14
No
No
Mmus\Setd2
1 of 14
No
No
Mmus\Setmar
1 of 14
No
No
Mmus\Suv39h1
1 of 14
No
No
Mmus\Suv39h2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\kmt5a
6 of 13
Yes
Yes
Xtro\ehmt2
1 of 13
No
No
Xtro\ezh2
1 of 13
No
No
Xtro\kmt2b
1 of 13
No
No
Xtro\nsd3
1 of 13
No
No
Xtro\prtn3
1 of 13
No
No
Xtro\rnd1
1 of 13
No
Yes
Xtro\setd1b
1 of 13
No
No
Xtro\setd2
1 of 13
No
No
Xtro\setdb1
1 of 13
No
No
Xtro\setmar
1 of 13
No
No
Xtro\suv39h2
1 of 13
No
No
Danio rerio (Zebrafish) (28)
Drer\kmt5aa
8 of 14
Yes
Yes
Drer\kmt5ab
8 of 14
Yes
Yes
Drer\ash1l
1 of 14
No
No
Drer\ehmt1a
1 of 14
No
No
Drer\ehmt1b
1 of 14
No
No
Drer\ehmt2
1 of 14
No
No
Drer\ezh1
1 of 14
No
No
Drer\ezh2
1 of 14
No
No
Drer\kmt2a
1 of 14
No
No
Drer\kmt2ba
1 of 14
No
No
Drer\kmt2bb
1 of 14
No
No
Drer\kmt2ca
1 of 14
No
No
Drer\kmt2d
1 of 14
No
No
Drer\LOC101883274
1 of 14
No
No
Drer\LOC101885800
1 of 14
No
Yes
Drer\LOC103909690
1 of 14
No
Yes
Drer\LOC103910708
1 of 14
No
Yes
Drer\nsd2
1 of 14
No
No
Drer\nsd3
1 of 14
No
No
Drer\setd1a
1 of 14
No
No
Drer\setd1ba
1 of 14
No
No
Drer\setd2
1 of 14
No
No
Drer\setmar
1 of 14
No
No
Drer\si:dkey-23a23.2
1 of 14
No
Yes
Drer\si:dkey-199k11.6
1 of 14
No
Yes
Drer\si:dkeyp-87d1.1
1 of 14
No
Yes
Drer\suv39h1a
1 of 14
No
No
Drer\suv39h1b
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (15)
Cele\set-1
8 of 14
Yes
Yes
Cele\mes-4
1 of 14
No
No
Cele\set-2
1 of 14
No
No
Cele\set-6
1 of 14
No
No
Cele\set-11
1 of 14
No
No
Cele\set-12
1 of 14
No
No
Cele\set-13
1 of 14
No
No
Cele\set-15
1 of 14
No
No
Cele\set-16
1 of 14
No
No
Cele\set-19
1 of 14
No
No
Cele\set-20
1 of 14
No
Yes
Cele\set-21
1 of 14
No
No
Cele\set-23
1 of 14
No
No
Cele\set-25
1 of 14
No
No
Cele\set-32
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP012481
7 of 12
Yes
Yes
Agam\AgaP_AGAP012610
3 of 12
No
Yes
Agam\AgaP_AGAP001535
1 of 12
No
No
Agam\AgaP_AGAP002246
1 of 12
No
No
Agam\AgaP_AGAP002741
1 of 12
No
No
Agam\AgaP_AGAP003013
1 of 12
No
No
Agam\AgaP_AGAP003597
1 of 12
No
No
Agam\AgaP_AGAP004656
1 of 12
No
No
Agam\AgaP_AGAP007978
1 of 12
No
No
Agam\AgaP_AGAP011192
1 of 12
No
No
Agam\AgaP_AGAP011688
1 of 12
No
No
Agam\AgaP_AGAP012516
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (23)
Atha\ASHH1
1 of 13
Yes
No
Atha\ASHH3
1 of 13
Yes
No
Atha\ASHH4
1 of 13
Yes
No
Atha\ATX1
1 of 13
Yes
No
Atha\ATX2
1 of 13
Yes
No
Atha\CLF
1 of 13
Yes
No
Atha\EFS
1 of 13
Yes
No
Atha\MEA
1 of 13
Yes
No
Atha\SDG4
1 of 13
Yes
No
Atha\SDG14
1 of 13
Yes
No
Atha\SDG16
1 of 13
Yes
No
Atha\SDG20
1 of 13
Yes
No
Atha\SDG21
1 of 13
Yes
No
Atha\SDG25
1 of 13
Yes
No
Atha\SDG29
1 of 13
Yes
No
Atha\SUVH1
1 of 13
Yes
No
Atha\SUVH3
1 of 13
Yes
No
Atha\SUVH4
1 of 13
Yes
No
Atha\SUVH5
1 of 13
Yes
No
Atha\SUVH6
1 of 13
Yes
No
Atha\SUVH7
1 of 13
Yes
No
Atha\SUVR5
1 of 13
Yes
No
Atha\SWN
1 of 13
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SET1
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\clr4
1 of 12
Yes
No
Spom\set1
1 of 12
Yes
No
Spom\set2
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Set8. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (11)
ash1
2 of 13
CG4565
2 of 13
G9a
2 of 13
Set1
2 of 13
Set2
2 of 13
Su(var)3-9
2 of 13
trr
2 of 13
trx
2 of 13
E(z)
1 of 13
egg
1 of 13
NSD
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KMT5A; lysine methyltransferase 5A
    7 of 14
    607240
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Dmel gene
        Ortholog showing functional complementation
        Supporting References
        Set8
        Hsap\KMT5A
        (Crain et al., 2022)
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Set8
        enhanceable
        mei-41
        (Sakaguchi and Steward, 2007)
        Set8
        suppressible
        mei-41
        (Sakaguchi and Steward, 2007)
        Set8
        suppressible
        Set8::Hsap\KMT5A
        (Crain et al., 2022)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-55
        Cytogenetic map
        88B3-88B3
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        88B3-88B3
        Limits computationally determined from genome sequence between P{lacW}trxj14A6&P{PZ}trx00347 and P{PZ}cv-c06951
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        88C1-88C10
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        88C1-88C10
        (BDGP Project Members, 1994-1999)
        88C-88C
        (determined by in situ hybridisation)
        (Cooley et al., 1988)
        Experimentally Determined Recombination Data
        Location

        3-55

        (FlyBase, 2016)

        3-53.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (12)
        Genomic Clones (18)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (52)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Huang et al., 2021)
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              Monomethylated H4K20 is strongly reduced in pr-set7 mutant larval brains.

              (Sakaguchi and Steward, 2007)

              Double-stranded breaks are not increased in pr-set7 mutants.

              (Sakaguchi and Steward, 2007)

              The DNA damage checkpoint, especially the ATR is pathway, is activated in pr-set7 mutants.

              (Sakaguchi and Steward, 2007)

              The DNA repair pathway is activated after γ-irradiation in pr-set7 mutants.

              (Sakaguchi and Steward, 2007)

              pr-set7 mutations suppress position effect variegation.

              (Karachentsev et al., 2005)

              A mutation in pr-set7 decreases levels of H4-K20 methylation in vivo and causes lethality.

              (Ketel et al., 2003)

              Disruption of pr-set7 reduces levels of His4 K20 methylation.

              (Fang et al., 2002)

              Disruption of pr-set7 results in lethality.

              (Fang et al., 2002)
              Relationship to Other Genes
              Source for database merge of
              Additional comments
              Nomenclature History
              Source for database identify of

              Source for identity of: pr-set7 CG3307

              (Fang et al., 2002)

              Source for identity of: pr-set7 l(3)neo41

              (Ketel, 2003.2.10)

              Source for identity of: Set8 pr-set7

              (FlyBase, 2009-)

              Source for identity of: PR-Set7 CG3307

              (Nishioka et al., 2002)

              Source for identity of: Set8 PR-Set7

              (Crain et al., 2022)
              Nomenclature comments

              CG3307 has ben renamed from 'PR-Set7' to 'Set8' to reflect the fact that the gene model does not contain a PR domain, and to prevent any confusion over orthology (CG3307 is orthologous to the human SETD8/KMT5A gene).

              (Crain et al., 2022)
              Etymology
              Synonyms and Secondary IDs (20)
              Reported As
              Symbol Synonym
              CG3307
              (Hosono et al., 2015, Nikalayevich and Ohkura, 2015, Ni et al., 2011.7.19, Müller et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Butler et al., 2003, Nishioka et al., 2002)
              PR-SET7
              (Ho et al., 2014, Scharf et al., 2009)
              PR-Set7
              (Richards et al., 2022, Pang et al., 2021, Yamaguchi et al., 2021, Lu et al., 2019, Song et al., 2018, Li et al., 2016, Lv et al., 2016, Comoglio and Paro, 2014, Sakaguchi et al., 2012, Tokusumi et al., 2012, Yang et al., 2008, Li et al., 2007, Sakaguchi and Steward, 2007, Sakaguchi and Steward, 2007, Karachentsev et al., 2005, Karachentsev et al., 2005)
              PR/SET07
              (Zhimulev and Belyaeva, 2003)
              PRSET7
              (Greer and Shi, 2012)
              PRSet7
              (Rennie et al., 2018)
              Pr-set-7
              (Bai et al., 2007)
              Pr-set7
              (Gujar and Wang, 2025, Gulati et al., 2020)
              PrSet7
              (Sahashi et al., 2014)
              SET8
              (Dillon et al., 2005)
              Set7
              (Sakaguchi et al., 2012, Karachentsev and Steward, 2004, Nishioka, 2003.4.24)
              Set8
              (Crain et al., 2024, Nikalayevich and Ohkura, 2015, Schotta et al., 2004, Ketel, 2003.2.10)
              dPR-Set7
              (Meier et al., 2012)
              dSET8
              (Ketel et al., 2003)
              dSet8
              (Endo et al., 2021, Fang et al., 2002)
              l(3)neo41
              (Spradling et al., 1999, BDGP Project Members, 1994-1999, Cooley et al., 1988)
              pr-Set7
              (Minakhina and Steward, 2006)
              pr-set7
              (Huang et al., 2021, Kim and Choe, 2014, Debruhl et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Gan et al., 2010, Müller et al., 2010)
              Name Synonyms
              PR/SET domain containing protein 7
              SET domain containing 8
              pr-set7
              Secondary FlyBase IDs
              • FBgn0038222
              Datasets (1)
              Study focus (1)
              Experimental Role
              Project
              Project Type
              Title
              • bait_protein
              modENCODE_chromatin_proteins_cells
              Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 55 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              References (89)