FB2026_03 , released September 17, 2026
Allele: Dmel\bezCR70056-KO-kG4-X
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General Information
Symbol
Dmel\bezCR70056-KO-kG4-X
Species
D. melanogaster
Name
FlyBase ID
FBal0376396
Feature type
allele
Associated gene
Dmel\bez
Associated Insertion(s)
TI{KozakGAL4}bezCR70056-KO-kG4-X
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{KozakGAL4}bezCR70056-KO-kG4-X
Cytology
Description

A TI{KozakGAL4} DNA cassette has been inserted into CG3829, replacing the coding sequence (coordinates of deleted sequence are 2R:24988184..24990307 , release 6 genome). This results in a simultaneous knock-out of CG3829 plus a knock-in of GAL4. The cassette is not predicted to trap CG3829 or express GAL4 because the insertion was mistakenly targeted to an intron in the 5'UTR rather than an exon in the 5'UTR. Weak GAL4 expression seen in the third instar larval brain may be non-specific. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: TCTGTCGCTGCACGCTAAAATGG and GCCCTTAACTAAACAACTAGTGG.

(Gene Disruption Project members, 2018-)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
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      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
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      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG3829CR70056-KO-kG4-X
      bezCR70056-KO-kG4-X
      Name Synonyms
      Secondary FlyBase IDs
        References (2)