FB2026_03 , released September 17, 2026
Allele: Zzzz\CamKII-IPpaAIP2.UAS
Open Close
General Information
Symbol
Zzzz\CamKII-IPpaAIP2.UAS
Species
a. artificial
Name
FlyBase ID
FBal0376583
Feature type
allele
Associated gene
Zzzz\CamKII-IP
Associated Insertion(s)
Carried in Construct
P{UAS-paAIP2}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Thornquist et al., 2020)
Progenitor genotype
Carried in construct
P{UAS-paAIP2}
Cytology
Description

UAS regulatory sequences drive expression of 'paAIP2', a genetically encoded light-inducible inhibitor of CaMKII kinase activity. paAIP2 is composed of the autocamtide-2 related inhibitory peptide 2 (AIP2) sequence (KKKLRRQEAFDAL), tagged at the N-terminal end with the light-sensitive Tag:AsLOV2 domain. In the absence of photoactivation, the Tag:AsLOV2 domain sterically blocks the action of AIP2. Upon illumination with blue light, the Tag:AsLOV2 domain undergoes a conformational change that allows the AIP2 sequence to act as a CaMKII kinase inhibitor. The conformational change is reversible, so that if the blue light illumination is removed, the Tag:AsLOV2 domain spontaneously reverts to the conformation that sterically blocks AIP2 (details from PMID:28318784).

(Thornquist et al., 2020)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Zzzz\CamKII-IP
Tagged with
Tag:AsLOV2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Zzzz\CamKII-IPpaAIP2.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)