FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-paAIP2}
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General Information
Symbol
P{UAS-paAIP2}
FlyBase ID
FBtp0151919
Feature type
transgenic_transposable_element
Component Allele(s)
Zzzz\CamKII-IPpaAIP2.UAS
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Zzzz\CamKII-IPpaAIP2.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Zzzz\CamKII-IP
Tagged with
Tag:AsLOV2
Description

UAS regulatory sequences drive expression of 'paAIP2', a genetically encoded light-inducible inhibitor of CaMKII kinase activity. paAIP2 is composed of the autocamtide-2 related inhibitory peptide 2 (AIP2) sequence (KKKLRRQEAFDAL), tagged at the N-terminal end with the light-sensitive Tag:AsLOV2 domain. In the absence of photoactivation, the Tag:AsLOV2 domain sterically blocks the action of AIP2. Upon illumination with blue light, the Tag:AsLOV2 domain undergoes a conformational change that allows the AIP2 sequence to act as a CaMKII kinase inhibitor. The conformational change is reversible, so that if the blue light illumination is removed, the Tag:AsLOV2 domain spontaneously reverts to the conformation that sterically blocks AIP2 (details from PMID:28318784).

(Thornquist et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-paAIP2}
(FlyBase, 1992-)
UAS-paAIP2
(Thornquist et al., 2020)
Secondary FlyBase IDs
    References (3)