FB2026_03 , released September 17, 2026
Allele: Spyo\Cas9H840A.nanos
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General Information
Symbol
Spyo\Cas9H840A.nanos
Species
S. pyogenes
Name
FlyBase ID
FBal0388195
Feature type
allele
Associated gene
Spyo\Cas9
Associated Insertion(s)
Carried in Construct
P{nanos-Cas9.H840A}
Transgenic product class
missense_variant
(Ren et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ren et al., 2014)
Mutagen
in vitro construct
(Ren et al., 2014)
Progenitor genotype
Carried in construct
P{nanos-Cas9.H840A}
Cytology
Description

nanos regulatory sequences (~700bp promoter sequence, the 5'UTR and the 3'UTR) regulate expression of Spyo\Cas9 carrying a H840A amino acid replacement. This mutation is predicted to inactivate the HNH domain and should result in the encoded protein acting as a nickase that cleaves a single DNA strand when targeted to a genomic site of interest using sgRNA, however only very low levels of mutagenesis were seen when this transgene was used in combination with two sgRNAs targeting a region of interest.

(Ren et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Spyo\Cas9
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Spyo\Cas9H840A.nanos
      Name Synonyms
      Secondary FlyBase IDs
        References (1)