FB2026_03 , released September 17, 2026
Allele: Dmel\shgTS-FRET
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General Information
Symbol
Dmel\shgTS-FRET
Species
D. melanogaster
Name
FlyBase ID
FBal0388508
Feature type
allele
Associated gene
Dmel\shg
Associated Insertion(s)
TI{TI}shgTS-FRET
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Eder et al., 2017)
Progenitor genotype
TI{TI}shgGX23w-
(Eder et al., 2017)
Associated Insertion(s)
TI{TI}shgTS-FRET
Cytology
Description

A tension-sensing module has been inserted into the coding sequence of the endogenous shg locus, in the cytoplasmic domain between the transmembrane domain and the β-catenin binding domain. The tension-sensing module consists of ECFP (FRET donor) and mEYFP (FRET acceptor) connected by an elastic linker (GPGGA)[[8]], derived from spider silk. If the tension is low, the two fluorophores are close enough to allow FRET. With increased tension, the elastic linker stretches, increasing the distance between the two fluorophores and reducing FRET.

(Eder et al., 2017)
Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      shgTS-FRET
      Name Synonyms
      Secondary FlyBase IDs
        References (1)