A TI{KozakGAL4} DNA cassette has been inserted into Rae1, replacing the coding sequence (coordinates of deleted sequence are 2R:21658844..21659985 , release 6 genome). This results in a simultaneous knock-out of Rae1 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Rae1 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: CACTGCTATGCACTCTTCATTGG and CAAGCCGCGCATTAACTGATCGG. The 3' end of the deletion associated with the insertion is 67 bp downstream of the 3' end of the ND-B12 gene and may affect the proper 3' end formation of its transcripts.