FlyBase Allele Report: Dmel\TyrRS[NIG.4561R]

General Information

Symbol

Dmel\TyrRS^NIG.4561R

Species

D. melanogaster

Name

FlyBase ID

FBal0391983

Feature type

allele

Associated gene

Dmel\TyrRS

Associated Insertion(s)

Carried in Construct

P{NIG.4561R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21, Arsham and Neufeld, 2009, Storkebaum et al., 2009)

Progenitor genotype

Carried in construct

P{NIG.4561R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-4561R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size | cell autonomous | somatic clone, with Scer\GAL4^Act5C.PP

(Arsham and Neufeld, 2009)

Phenotype Manifest In

lysosome & larval fat body | somatic clone | cell autonomous, with Scer\GAL4^Act5C.PP

(Arsham and Neufeld, 2009)

scutellar bristle, with Scer\GAL4^sca.PU

(Storkebaum et al., 2009)

Detailed Description

Statement

Reference

Aats-tyr^{dsRNA.Scer\UAS.NIG} clones in fat bodies from well-fed early third instar larvae (generated using Scer\GAL4^Act5C.PP) accumulate lysosomes, as indicated by elevated LysoTracker staining, and comprise small cells with small nuclei.

(Arsham and Neufeld, 2009)

Flies expressing Aats-tyr^NIG.4561R under the control of Scer\GAL4^sca.PU have bristle defects; scutellar bristles are often shorter than normal or missing. The dorsal scutellar bristles show more severe defects than the anterior scutellar bristles.

(Storkebaum et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sca.PU, TyrRS^NIG.4561R has scutellar bristle phenotype, suppressible by Hsap\YARS1^WT.UAS, Scer\GAL4^sca.PU

(Storkebaum et al., 2009)

Scer\GAL4^sca.PU, TyrRS^NIG.4561R has scutellar bristle phenotype, suppressible by Hsap\YARS1^E196K.UAS, Scer\GAL4^sca.PU

(Storkebaum et al., 2009)

Scer\GAL4^sca.PU, TyrRS^NIG.4561R has scutellar bristle phenotype, suppressible by Hsap\YARS1^G41R.UAS, Scer\GAL4^sca.PU

(Storkebaum et al., 2009)

Scer\GAL4^sca.PU, TyrRS^NIG.4561R has scutellar bristle phenotype, suppressible by Hsap\YARS1^{153-156delVKQV.UAS}, Scer\GAL4^sca.PU

(Storkebaum et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The bristle defects caused by expression of Aats-tyr^NIG.4561R under the control of Scer\GAL4^sca.PU are completely rescued by co-expression of Hsap\YARS^Scer\UAS.cSa.

The bristle defects caused by expression of Aats-tyr^NIG.4561R under the control of Scer\GAL4^sca.PU are completely rescued by co-expression of Hsap\YARS^{E196K.Scer\UAS}.

The bristle defects caused by expression of Aats-tyr^NIG.4561R under the control of Scer\GAL4^sca.PU are not rescued by co-expression of Hsap\YARS^{G41R.Scer\UAS}.

The bristle defects caused by expression of Aats-tyr^NIG.4561R under the control of Scer\GAL4^sca.PU are not rescued by co-expression of Hsap\YARS^{153-156delVKQV.Scer\UAS}.

(Storkebaum et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

NIG-Fly

4561R-2

P{NIG.4561R}2

Notes on Origin

Discoverer

Comments

Expression of Aats-tyr^{dsRNA.Scer\UAS.NIG} produces dsRNA which results in dsRNA interference (RNAi) of the Aats-tyr gene. Aats-tyr^{dsRNA.Scer\UAS.NIG} was generated by the National Institute of Genetics of Japan in Shizuoka.

(Arsham and Neufeld, 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Aats-tyr^NIG.4561R

Aats-tyr^{dsRNA.Scer\UAS.NIG}

TyrRS^NIG.4561R

TyrRS^RNAi.UAS.NIG

TyrRS^{dsRNA.Scer\UAS.NIG}

TyrRS^{dsRNA.UAS.NIG}

Name Synonyms

Secondary FlyBase IDs

FBal0230412
FBal0230481

References (6)

Report Sections

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