FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\ImpKH3-4
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General Information
Symbol
Dmel\ImpKH3-4
Species
D. melanogaster
Name
FlyBase ID
FBal0392026
Feature type
allele
Associated gene
Dmel\Imp
Associated Insertion(s)
P{PTT-un}G00080
Carried in Construct
Also Known As
GFP-Imp, G080-GFP-Imp
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Vazquez-Pianzola and Suter, 2023.7.14)
Progenitor genotype
P{PTT-un}G00080
(Vazquez-Pianzola and Suter, 2023.7.14)
Associated Insertion(s)
P{PTT-un}G00080
Cytology
Description

Substitution of GDDG for GTRG in the Imp KH3 RNA-binding domain plus substitution of GDDG for GKGG in the KH4 RNA-binding domain. Induced on the P{PTT-un}G00080 chromosome; the inserted protein trap element is still present.

(Vazquez-Pianzola and Suter, 2023.7.14)
Allele components
Component
Use(s)
Inserted element
P{PTT-un}
Tagged with
EGFP
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
X:10,798,070..10,798,075 [-]
Inserted_sequence:

GACGAT

Comment:

The substitution of 6 nucleotides (ACCAGG to GACGAT) results in the substitution of GDDG for GTRG in the Imp KH3 RNA-binding domain. One of two mutations in strain. There is also a substitution of GDDG for GKGG in the Imp KH4 RNA-binding domain.

(Vazquez-Pianzola and Suter, 2023.7.14)
MNV
X:10,797,640..10,797,645 [-]
Inserted_sequence:

GACGAT

Comment:

The substitution of 6 nucleotides (AAGGGT to GACGAT) results in the substitution of GDDG for GKGG in the Imp KH4 RNA-binding domain. One of two mutations in strain. There is also a substitution of GDDG for GTRG in the Imp KH3 RNA-binding domain.

(Vazquez-Pianzola and Suter, 2023.7.14)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
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      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ImpKH3-4
      (Vazquez-Pianzola and Suter, 2023.7.14)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)