FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\RdlA2'P-T6'I
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General Information
Symbol
Dmel\RdlA2'P-T6'I
Species
D. melanogaster
Name
FlyBase ID
FBal0399326
Feature type
allele
Associated gene
Dmel\Rdl
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Guo et al., 2023)
gene targeting by homologous recombination
(Guo et al., 2023)
Progenitor genotype
TI{TI}Rdl3xP3RFP
(Guo et al., 2023)
Cytology
Description

CRISPR/Cas9-mediated homology-directed repair has resulted in a chromosome with a Rdl locus that carries an A to P point mutation in the 2' position of the M2 membrane-spanning region and a T to I point mutation in the 6' position of the M2 membrane-spanning region.

(Guo et al., 2023)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:9,155,151..9,155,151 [-]
Nucleotide change:

C9155151T

Amino acid change:

T305I | Rdl-PA; T305I | Rdl-PC; T305I | Rdl-PD; T305I | Rdl-PE; T305I | Rdl-PF; T305I | Rdl-PG

Reported amino acid change:

T?I

Comment:

A T to I amino acid substitution was introduced in the 6' position of the M2 membrane-spanning region of Rdl by CRISPR/Cas9-mediated homology-directed repair. An A to P substitution at position 2' of the same domain was also introduced in the line. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change. Nucleotide change not specified.

(Guo et al., 2023)
point_mutation
3L:9,155,164..9,155,164 [-]
Nucleotide change:

G9155164C

Amino acid change:

A301P | Rdl-PA; A301P | Rdl-PC; A301P | Rdl-PD; A301P | Rdl-PE; A301P | Rdl-PF; A301P | Rdl-PG

Reported amino acid change:

A?P

Comment:

A A to P amino acid substitution was introduced in the 2' position of the M2 membrane-spanning region of Rdl by CRISPR/Cas9-mediated homology-directed repair. AT to I substitution at position 6' of the same domain was also introduced in the line. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change. Nucleotide change not specified.

(Guo et al., 2023)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
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      Disease
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      Modifiers Based on Experimental Evidence ( 0 )
      Disease
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      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      RdlA2'P-T6'I
      (Guo et al., 2023)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)