FB2026_03 , released September 17, 2026
Allele: Spyo\Cas9xCas9(3.7)-BE3.nanos
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General Information
Symbol
Spyo\Cas9xCas9(3.7)-BE3.nanos
Species
S. pyogenes
Name
FlyBase ID
FBal0400845
Feature type
allele
Associated gene
Spyo\Cas9
Associated Insertion(s)
Carried in Construct
P{nanos-xCas9(3.7)-BE3}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Thakkar et al., 2023)
Progenitor genotype
Carried in construct
P{nanos-xCas9(3.7)-BE3}
Cytology
Description

The nanos promoter drives expression of 'xCas9(3.7)-BE3', a base editor that targets cytosine in DNA; it favors the conversion of C to T. The xCas9(3.7)-BE3 sequence (from Addgene plasmid 108380) encodes a fusion protein composed of 1. a modified rat Apobec1 cytidine deaminase ( RGDID:2133 ), 2. x-Cas9(3.7), an engineered Cas9 nickase (contains the D10A mutation) that contains mutations broadening the PAM compatibility to GAA, GAT, and NG in addition to its canonical NGG, and 3. the uracil DNA glycosylase inhibitor (UGI) from a Bacillus subtilis bacteriophage (see PMID:29512652).

(Thakkar et al., 2023)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Spyo\Cas9
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Spyo\Cas9xCas9(3.7)-BE3.nanos
      Name Synonyms
      Secondary FlyBase IDs
        References (1)