FlyBase Allele Report: Dmel\amls[RNAi.5xUAS.3-ORF]

General Information

Symbol

Dmel\amls^{RNAi.5xUAS.3-ORF}

Species

D. melanogaster

Name

FlyBase ID

FBal0404335

Feature type

allele

Associated gene

Dmel\amls

Associated Insertion(s)

Carried in Construct

M{5xUAS-amls.RNAi.3-ORF}

Key Links

Transgenic product class

RNAi_reagent

(Reim et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Reim et al., 2014)

Mutagen

in vitro construct

(Reim et al., 2014)

Progenitor genotype

Carried in construct

M{5xUAS-amls.RNAi.3-ORF}

Cytology

Description

5xUAS regulatory sequences drive expression of a short hairpin RNA (shRNA) that targets the als open reading frame (target site: CGGACGAAAGCCTGGATTTTA).

(Reim et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

amls

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^C-765

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^nub.PU

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^salm.E

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^vg.PU

(Reim et al., 2014)

Phenotype Manifest In

wing margin, with Scer\GAL4^C-765

(Reim et al., 2014)

wing margin, with Scer\GAL4^nub.PU

(Reim et al., 2014)

wing margin, with Scer\GAL4^salm.E

(Reim et al., 2014)

wing margin, with Scer\GAL4^vg.PU

(Reim et al., 2014)

wing sensillum, with Scer\GAL4^C-765

(Reim et al., 2014)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, amls^{RNAi.5xUAS.3-ORF} has visible | adult stage phenotype, enhanceable by lgs^17E.UAS/Scer\GAL4^nub.PU

(Reim et al., 2014)

Scer\GAL4^C-765, amls^{RNAi.5xUAS.3-ORF} has visible | adult stage phenotype, enhanceable by Scer\GAL4^C-765/pan^RNAi.UAS.cUa

(Reim et al., 2014)

Scer\GAL4^salm.E, amls^{RNAi.5xUAS.3-ORF} has visible | adult stage phenotype, enhanceable by Scer\GAL4^salm.E/sgg^UAS.cUa

(Reim et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, amls^{RNAi.5xUAS.3-ORF} has wing margin phenotype, enhanceable by lgs^17E.UAS/Scer\GAL4^nub.PU

(Reim et al., 2014)

Scer\GAL4^C-765, amls^{RNAi.5xUAS.3-ORF} has wing margin phenotype, enhanceable by Scer\GAL4^C-765/pan^RNAi.UAS.cUa

(Reim et al., 2014)

Scer\GAL4^salm.E, amls^{RNAi.5xUAS.3-ORF} has wing margin phenotype, enhanceable by Scer\GAL4^salm.E/sgg^UAS.cUa

(Reim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

amls^{RNAi.5xUAS.3-ORF} is rescued by amls^{ΔUBX.3-ORF-RR.UAS}/Scer\GAL4^vg.PU

(Reim et al., 2014)

Not rescued by

amls^{RNAi.5xUAS.3-ORF} is not rescued by amls^ΔN.UAS/Scer\GAL4^vg.PU

(Reim et al., 2014)

amls^{RNAi.5xUAS.3-ORF} is not rescued by amls^ΔPUG.UAS/Scer\GAL4^vg.PU

(Reim et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

als^{RNAi.5xUAS.3-ORF}

amls^{RNAi.5xUAS.3-ORF}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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