UAS sequences regulate expression of wild-type als that is tagged at the N-terminal end with 3xTag:HA. Seven conservative base pair mutations have been introduced, to render the construct insensitive to RNAi mediated by either M{10xUAS-als.RNAi.3-ORF} or M{5xUAS-als.RNAi.3-ORF}.
amlsUAS.3-ORF-RR.N-Tag:HA/Scer\GAL4vg.PU partially rescues amlsRNAi.10xUAS.3-ORF
amlsUAS.3-ORF-RR.N-Tag:HA/Scer\GAL4ey.PU partially rescues amlsRNAi.10xUAS.3-ORF