UASp sequences regulate expression of a deleted form of the Spc105R B isoform which lacks amino acids 123-1285. The coding sequence is tagged at the C-terminal end with 6xTag:MYC. Silent mutations have been introduced to render the transgene resistant to RNAi mediated by the P{TRiP.GL00392} transgene.
Scer\GAL4αTub84B.PL/Spc105RΔM.UASp.RR fails to rescue Spc105RGL00392