The 'SEED-mCh' cassette in TI{TI}boiSEED-mCh has been removed by 'harvesting' (using Cas9 together with 'gRNA#1' (sequence GTAGTACGATCATAACAACG) and 'gRNA#2' (sequence GTACATCCATACAGTACCAG) to trigger a single-strand annealing repair event), reconstituting a functional mCherry tag in the boi locus.