FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\LanB1R1059X.UAS.Tag:HA
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General Information
Symbol
Dmel\LanB1R1059X.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0408753
Feature type
allele
Associated gene
Dmel\LanB1
Associated Insertion(s)
Carried in Construct
PBac{UAS-LanB1.R1059X.HA}
Key Links
Genomic Maps

Transgenic product class
stop_gained
(Yasuda et al., 2025)
Mutagen
Nature of the Allele
Transgenic product class
stop_gained
(Yasuda et al., 2025)
Mutagen
in vitro construct
(Yasuda et al., 2025)
Progenitor genotype
Carried in construct
PBac{UAS-LanB1.R1059X.HA}
Cytology
Description

UASt regulatory sequences drive expression of the LanB1 open reading frame, mutated to carry a R1059X amino acid substitution. This change is analagous to a K1049Pfs7 change in the orthologous human LAMB1 gene, a pathogenic variant associated with LAMB1-related leukoencephalopathy. The coding sequence is tagged at the C-terminal end with three copies of Tag:HA. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Yasuda et al., 2025)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
LanB1
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
2L:7,818,198..7,818,199 [+]
Nucleotide change:

CGT7818198TAG

Amino acid change:

R1059term | LanB1-PA; R1059term | LanB1-PB

Reported amino acid change:

R1059term

Comment:

Analogous mutation in human LAMB1 implicated in LAMB1-related leukoencephalopathy and lissencephaly; mutation carried on in vitro construct; site and nature of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Yasuda et al., 2025)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Yasuda et al., 2025)
      LAMB1:p.Lys1049Ter
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      Comments concerning this variant
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      LanB1R1059X.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)