FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\RELAvg-2A-p65AD.attP.FRT
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General Information
Symbol
Hsap\RELAvg-2A-p65AD.attP.FRT
Species
H. sapiens
Name
FlyBase ID
FBal0409747
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
TI{2A-p65(AD)::Zip+}vg2A-p65AD.attP.FRT
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Soffers et al., 2025)
gene targeting by homologous recombination
(Soffers et al., 2025)
Progenitor genotype
Associated Insertion(s)
TI{2A-p65(AD)::Zip+}vg2A-p65AD.attP.FRT
Cytology
Description

A Trojan exon encoding a T2A peptide followed by the TI{2A-p65(AD)::Zip+} hemidriver has been inserted in an intron of vg. The T2A-TI{2A-p65(AD)::Zip+} sequence is flanked by a pair of FRT sites, which in turn are flanked by a pair of inverted attP sites. This allows for excision of the T2A-TI{2A-p65(AD)::Zip+} exon (using FLP recombinase) or for replacement of the sequence between the attP sites with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase.

(Soffers et al., 2025)
Allele components
Component
Use(s)
Inserted element
TI{2A-p65(AD)::Zip+}
Encoded product / tool
p65(AD)::Zip+
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAvg-2A-p65AD.attP.FRT
      Name Synonyms
      Secondary FlyBase IDs
        References (1)