UASt regulatory sequences drive expression of the sl open reading frame (amplified from the RE62235 cDNA clone), tagged at the C-terminal end with three copies of Tag:HA. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
wing, with Scer\GAL4nub.PK
slUAS.Tag:HA rescues slCR01192-TG4.0