The RMCE cassette of the TI{CRIMIC.TG4.1}Ack-likeCR01407-TG4.1 insertion has been replaced with a TI{FFL.1} cassette that is in gene-trap (GT) orientation relative to Ack-like. This is expected to truncate the Ack-like protein and to result in the expression of a separate mCherry protein under the control of endogenous Ack-like regulatory sequences.