FB2026_03 , released September 17, 2026
Allele: Avic\GFPspGFP1-10.D4H.UAS.Tag:FLAG,Tag:ER(cb5)
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General Information
Symbol
Avic\GFPspGFP1-10.D4H.UAS.Tag:FLAG,Tag:ER(cb5)
Species
A. victoria
Name
FlyBase ID
FBal0414393
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{UAS-D4H-GFP11x7-V5-P2A-FLAG-GFP(1-10)-ER}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wang et al., 2026)
Progenitor genotype
Carried in construct
P{UAS-D4H-GFP11x7-V5-P2A-FLAG-GFP(1-10)-ER}
Cytology
Description

UASt sequences regulate expression of a split-GFP sensor that can be used to monitor cholesterol and endoplasmic reticulum (ER) contact. The construct contains a dicistronic transcription unit that encodes two open reading frames (ORFs) separated by a 2A linker (this promotes separate translation of each protein). The first ORF comprises Tag:D4H(Y415A.A463W) fused to seven tandem copies of the spGFP11 split fluorescent protein fragment, followed by Tag:V5. The second ORF comprises Tag:FLAG fused to spGFP1-10 followed by a Tag:ER(cb5) ER targeting motif.

(Wang et al., 2026)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spGFP1-10
Tagged with
Tag:ER(cb5)
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Avic\GFPspGFP1-10.D4H.UAS.Tag:FLAG,Tag:ER(cb5)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)