FB2026_02 , released June 18, 2026
Clone: Dmel\AF083318
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General Information
Symbol
Dmel\AF083318
Species
D. melanogaster
Name
AF083318
FlyBase ID
FBcl0000595
Feature type
cDNA_clone
Computed gene(s)
Dmel\LeuRS
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    AF_cDNA
    • AF cDNA library of D. melanogaster, Canton-S strain, adult brain.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult brain
      (Posey et al., 2001)
      Sequence Data of the Insert
      Partial Sequence
      Total bases
      1,420
      NCBI
      AF083318
      Partial sequence dataSequence Downloader
      CCAATTTCGTGGAGAGCACTGCGGATGCTGGTATTCTGCGTCTATACACTTTCATTGAGTGGGTCAAAGAGATGTTGGAA
AACAGGAGCAGTCTGAGAAAGGGCACTGATAAAACCTTCAACGATCAAGTTTTCCTGAGTGAACTCAATTTGAAAACGCA
GCAAACCGATGAGAATTACCGAAAGATGTTGTTCAAGGAGGCTCTAAGGAGTGGCTTCTACGAACTGCAGTTGGCCAGGG
ACAAGTACAGGGAGCTGTGTGGCGCAAATGGAATGCATGAAGATCTGGTTCTGGAGTTCATTCGCAGACAAGCTCTTTTG
GTATCCCCTATTTGCCCCCATATGGCGGAGCATGTCTGGGGACTGCTGGGTAACAAGGAGTCCATTGTTCATGCTCGGTG
GCCAGAAGTGGGAGCCATCAACGAAGTCGATATCCTGTGCTCGGAGTACCTCATGGAAGCCGCTCACTCGTTCCGTCTGA
ACCTCAAGAACTTGCTGCAAATCAAGGGCAAGGCCGGAAAAGATAAATCTGTGAATGTCCAGGCAGCGAAACCGAATCGA
GGGCTCGTTTGGGTCGCCAAGACCTATCCTCCTTGGCAGTGCTGTGTCCTCGATACCATGAAGGAGTTGTTTAACAAATC
GCAGGCCCTTCCAGATAACAAAGTCATCGCTGCTACACTGCAGCAAAAGGCCGAGTTAAAGAAGTTCATGAAACGAGTGA
TGCCCTTTGCCCAGATGATTCGCGAAAAGGTGGAGTCCGGCAAGGGTGTGGCTGCATTGGCCGTGACTTTGGAGTTCGAT
GAGCGTCAGGTGTTGATCAGCAATTTGGAGTACCTGAAGAACACACTGGATCTTGATGTCTTGGAAATTAAGTACACCGA
TGATCCGTCAGCGCCGGAGAAAACTCGTGAAGAAGTGCGTCCCGGCTCTCCCTTCATCAGCTTTTCAGTGGCCCCAAATG
TGAGCGTTGAGCTCACGAATCCTATCGAGCGCTCGTCGCTTTTCCAGGTCAACACTGTCATATCCGAGGGAGACACGGTC
CAATCTCTGCGAGAGAAGCTCTCCAAGATTATTGGACTTAAAACGGATGCGGCTACCCTTAAGATCTGGCGGTATGAGGA
TCCCGTTCTGGGACCCCGAAAAATTCCCAACTTTGAGGATTACAAAGCAGGAAAGTCTGTACTCGGCGAGGGAAACTTCG
TTCTGGATGTGGAGAACAAGAAAGTGAGCCTGGAGCAGGCTGGAAAACTGACCGAAGTGGGAAGGAATCTGATCTATGTG
GTGGATTAGGTCAAAACGGCATCACAAGCCAAACTAACTTATTCATAGAATGAAATTAAAATGTTGTAAAACACAAATAA
TGGCTATTAAAATGTACTGTGAGTTGGTTGTGCCCAATTTAACTTACGATTAAAAAAAAA
      Library Information (1)
      Library: AF_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult brains. The strain used was not the iso-1 sequenced strain.

      (Posey et al., 2001)
      Sample preparation

      Tissue was dessicated using the Stratagene HybriZAP system.

      Protocol

      Library constructed from dessicated tissue using the Stratagene HybriZAP system. Library was designed for protein expression, and constructed such that full-length cDNAs containing 5' untranslated regions are unlikely to be present.

      (Posey et al., 2001)
      Comment

      GenBank AF083295-AF083548, AF171701-AF171760.

      (Posey et al., 2001)
      Reported As
      Symbol Synonyms
      AF
      AF_cDNA
      References (1)
      Research paper (1)
      Posey et al., 2001, Genome Biol. 2(3): RESEARCH0008
      Survey of transcripts in the adult Drosophila brain. [FBrf0135825]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)