FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT25963
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General Information
Symbol
Dmel\AT25963
Species
D. melanogaster
Name
AT25963
FlyBase ID
FBcl0004441
Feature type
cDNA_clone
Computed gene(s)
Dmel\spas
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI06043
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    seminal vesicle
    (Stapleton et al., 2002)
    testis
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,835
    NCBI
    BT001351
    Full Length sequence dataSequence Downloader
    CACTGGCTGGGACTCCAGAGCTTAGCTTCAATTTTCAACGTAGTGAGAGTTGTAAGTGCGGAAGAAAATCAGTACTCTCG
GATATCAGCAAAATGAGCACTAGCTCAAACGACGTACAACAATAATATTCGAGATTTGTTTGTTTTTTTGTGCGGCATCG
ATACAAACGCGTGCCACGGCCAACCGATTTGGCTTACAAGGATAATTTCCCCGTGCAACTTTCACGGGGTTAGGGCAAGG
AGAGCAGGGGGCCGGAGTGCTGGACGCGCAGACAGCGCGGAATCGATATCGATGGTACGCACTAAAAACCAGTCGTCCTC
CTCCAGCGCCAGCAGCAGCAGCACCAAGTCACCAATAAAGTCCAGCAGCGGGGCAGGATCATCCGGCGGAGGACTCGGTG
GTCGCCAGTCCACTCACCGCTCGTCGAGCGCCTCAAACGTTGCTGCCGTTGTTGCCGGCGGTTCATCTGCCGCCGGTGGA
GGCTCCTCATCGAATCGCCGTAGTCCGGGCAGCTCACCCGACGGCGACGACGACACCACCACCACAGATGACCTGACCCC
CACAACGTGCTCCCCACGCAGCGGCCACCACCATTCGTACGGCGGCTACTCGTCCTCTGTGCACAAACAGAACCTCTACG
TCGTCTCGTTCCCCATAATCTTTCTTTTCAACGTTCTGCGCTCGCTGATTTATCAGCTGTTTTGTATTTTTCGCTACCTA
TACGGTGCGAGCACAAAGGTGATATACCGCCCGCACCGACGCGACTGTAATATTGAAATCGTGGTGCAGAATTCCTCCAA
GGAGCAACAGCAGTCGTTGAACCATCCTTCGGAGCTGAACCGTGAAGGTGACGGACAGGAACAGCAGTTATCCAATCAGC
CACAGCGTTTCAGGCCCATTCAACCGTTGGAGATGGCCGCCAATCGTCCGGGAGGAGGGTATTCACCTGGTCCCGGCGAT
CCATTGCTGGCCAAGCAGAAGCATCACCATCGACGCGCCTTCGAGTACATCTCCAAGGCGCTCAAAATTGATGAGGAGAA
CGAAGGTCACAAAGAGCTGGCAATTGAGCTCTACCGAAAAGGTATCAAGGAGCTAGAGGATGGCATAGCTGTCGATTGCT
GGAGTGGACGTGGTGATGTGTGGGATCGGGCTCAGCGCCTGCACGACAAGATGCAGACAAACCTTTCGATGGCGCGGGAT
CGTCTCCATTTTCTAGCTCTGCGTGAGCAGGATTTGCAAATGCAGCGCCTCTCCTTAAAGGAGAAGCAGAAAGAAGAGGC
TCAAAGCAAGCCGCAGAAGACCAGGGAGCCCATGCTGGCAGGAATGACCAACGAACCAATGAAACTAAGGGTGCGCAGCA
GTGGCTATGGGCCCAAGGCCACCACCAGTGCCCAACCCACTGCATCTGGCCGCAAACTGACAATTGGATCCAAACGACCC
GTCAACTTGGCCGTGGCCAACAAATCACAAACGCTGCCCCGCAACCTTGGCTCCAAAACATCTGTAGGCGCAGTCCAAAG
GCAACCAGCCAAGACAGCTGCAACGCCTCCCGCCGTTCGGCGACAATTTTCTTCGGGGCGTAATACGCCGCCCCAGCGTT
CGCGGACTCCCATAAACAACAACGGACCCAGTGGCAGTGGCGCCAGCACTCCGGTGGTTAGTGTGAAGGGAGTGGAACAG
AAGCTGGTGCAACTTATACTCGACGAGATCGTCGAGGGTGGGGCCAAAGTGGAGTGGACTGACATTGCCGGTCAGGATGT
GGCCAAGCAGGCTCTACAGGAAATGGTCATTCTACCCTCCGTCCGACCGGAACTCTTTACGGGGCTTCGTGCACCGGCTA
AGGGTTTATTGCTGTTTGGTCCTCCCGGAAATGGCAAGACACTGCTGGCCCGCGCCGTGGCTACTGAGTGTAGTGCCACC
TTCCTGAACATTTCGGCTGCCTCGCTGACCAGCAAGTACGTGGGGGATGGCGAGAAACTGGTGAGGGCTCTCTTCGCCGT
GGCCCGTCACATGCAGCCCTCCATTATTTTCATCGACGAGGTGGACTCGTTGCTTTCAGAGCGAAGCAGCAGCGAGCATG
AAGCATCGCGTCGCCTAAAGACCGAGTTTCTAGTGGAGTTCGATGGGCTGCCTGGAAATCCAGACGGAGACAGGATCGTG
GTGCTGGCCGCCACCAATCGACCGCAGGAATTGGACGAGGCAGCCCTGCGTCGGTTCACAAAGCGCGTTTACGTCTCACT
GCCCGACGAGCAAACCCGCGAGCTGCTCCTCAATCGACTGCTCGAGAAGCAAGGCAGTCCGTTGGATACCGAGGCGTTGC
GTCGCCTTGCAAAGATAACAGACGGATACTCGGGCTCCGACTTAACGGCACTGGCCAAGGACGCCGCATTGGAGCCCATT
CGAGAGTTGAATGTGGAGCAAGTGAAATGTCTGGACATCAGTGCAATGCGTGCAATCACAGAGCAGGACTTTCACAGTTC
GCTCAAGCGCATCAGGCGCTCGGTGGCGCCGCAAAGCCTCAACTCATATGAGAAGTGGTCGCAAGATTATGGCGACATCA
CCATCTAGTCTGTTCTAGACAATGATCCTGCCCTCCTTCATCAATCCAATTTGGAGAAGCTGTGATGTGAGTCTCAGAGC
GCAACCCATTGTCGTTACACTAGTTTTGATTATGTGACCCATGTTTTTCGCCCTTAAGGTTCTATTCAATCCGTAGACTT
TTCGTTTCACAAACATTGTTACATTTGTATTTACCTGTGAGTTATTTAATATGATTTGTTTCCCAGCTAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)