FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT25289
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General Information
Symbol
Dmel\AT25289
Species
D. melanogaster
Name
AT25289
FlyBase ID
FBcl0040959
Feature type
cDNA_clone
Computed gene(s)
Dmel\stc
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,677
      NCBI
      BT125955
      Full Length sequence dataSequence Downloader
      GGATTGCGGACGATCCCGTGGTAGCGCTGGGAAATGCCCAGCAGAGGACGGCGGGCAGTTCGAGATTGTTCCGGCAAAGA
AGCAGGCGCAACTGCAACAGACGCTGGTCACCAACGACAGCACCGGACTCTCAATGGCTGAGTACTGGCAGCAGTTGACC
AACGGCGCCGGAGAAGCGGGACCCGGCAATGAGTCATCCGCCATGGCAGACTGCAACGGAGGTCACGAATCGGCGGCAGT
GGGTGGTTCCTGCAACAGGCACAGCAACAACAATTACGTGAACTTCAATCAGTTCATTATGCAGCACAATCTGGGTGGAG
GAGCGCCCTCGAATGCCACCAGCACTATGCAGCATCCGGTGGGCAGCAGCTACACTAACTTCTCCCTGGGCGGCGGTGGA
GGAGCCTTTGGACTGAACCCACCAGTGGCATCCGCGAGTACCAGCCACTTTGCAAATTTTTACTCGCAGGCCATGATCCC
AACCTATCAAAATGGCGATGGCTTCGCTAGAGTCACCGTCACCAGCAGCTATGGGTCGGTCAATCCGAGCAACAGCAATT
TTTCGAGTTTTTACACACCTTTTGGCAATAATCCGTTTGACTTCAGCGCTTCCAAGCTGCAGGCCTCGGCTCCGGAATTC
GTGCCCAATTTCGCCAAGCTGAGTCTAGAAGAGACACCGGCAGCCGCCACTACGAACGGAAACAGCACTGCCAGCCTAGA
GACTGCCATAAATAGACGAGACCTAGGACTCTGCGAGCCCAAGAACCAGCTGAACGAGGAGCTAACAACCAATGCTCGAA
CCATAATTACGAACGGGAGCGTGAGCGGGAACGTGACCGGGACCGGGACAGGGAAAGAGACCGGGACAGAGACAGGGACA
GAGACCGGGACAGAGACCGGGACAGGGACAGGGACTCTAGACCCGGAAATACGCGACAACAACGCCGCTCCGACTATCGG
GACGATCGGGAGGACCGGTACGAAAGAAGCGATCGTAGGAGGCCGCAGAAGCAGCAACGCTACGACAATCATCGCAGCAA
CAAACGCAGGGATGACTGGAATCGCAACAGGGACCGCATCAACGGCTTTCCCAGGGCCGTTGACGATTTGGACACGAGCA
ATGAGAGCGCACACCCATCGCCCGAAAAGCAGTCGCAACTGCAGCAAATCTCCCCAAGGCGAGGTCCACCGCTACCACCG
GCGGATAACGAAAAACTATCGCAGCGAGAGAAACTGGTTCGCGACATCGAACAGCGCCGATTGGAGTGCCTTGTATGCGT
GGAGGCGATCAAGTCGCACCAGCCAACCTGGTCGTGCAGGAACTGCTATCACATGCTGCACCTTAAGTGCACCATCACCT
GGGCGAGTAGCTCCAAGTCCGAGGTGGGCTGGCGTTGTCCAGCTTGCCAGAATGTGCTGCAGGATTTGCCACGCGACTAT
CTCTGCTTTTGCGGCAAACTGAAAAACCCGCCGGTATCACGGACCGAATTGGCCCACAGCTGTGGCGAGGTCTGCTGCCG
AATTGAGGGTTGTAGCCATGCCTGCACTCTGCTCTGTCACCCGGGGCCTTGCCCGCCGTGCCAGGCCAACGTGGTGCGCA
GCTGTGGATGTGGACGCAGCACAAAAACGATGCAGTGCGCCATGAAGGAAGAGGTGCTCTGCGGCGAGATCTGCGACAAG
CTGCTTAACTGTGGCGAGCATCGCTGCCAGGCGGAGTGCCACTCGGGCAAGTGCGCGGCGTGTTCCGAGCAAGTGGTGCA
GCAGTGCCACTGCGGCAAGCAGGAGCGCAAGGTGCCATGCACGCGAGAATCGCAGGACAAACGCACCTATTCGTGCAAGG
ACAGCTGCGGTCAACCGCTTCCCTGCGGCCACCACAAGTGCAAGGACTCCTGCCACGCGGGAAGTTGTAGACCCTGCAAG
CTGAGTCCCGAGCAGATAACAAGTTGTCCTTGTGGCAAAATGCCTGTACCGGCTGGCCAGCGTTCAAGCTGCCTGGACCC
GATTCCCACATGCGAGGGCATCTGCTCTAGGACATTACGTTGTGGAAAGCCAGCGCATCCGCATCAATGCGGAAGCAAAT
GTCACTTGGGCCAGTGCCCTCCATGTCCCAAGCAGACCGGTGTAAAGTGTCGTTGCGGCCACATGGACCAAATGATAAAG
TGTCGGCAGCTATGTAACAGAGCGGACGACGCCCGCTGCAAGCGACGTTGCACCAAGAAGCGCAGCTGTGGAAAGCACAA
GTGCAACGTTGAGTGCTGCATCGATATAGACCACGACTGTCCACTGCCATGTAATCGAACCCTCAGTTGTGGCAAACACA
AGTGCGATCAGCCGTGTCACCGCGGCAATTGTCCGCCATGCTACCGCAGCAGTTTCGAAGAGCTCTATTGCGAGTGTGGT
GCCGAAGTGATCTATCCACCAGTGCCGTGTGGCACCAAGAAGCCAATCTGCAAGCTTCCTTGCTCGCGCATTCATCCATG
CGATCATCCGCCGCAGCACAACTGCCATTCGGGCCCCACCTGTCCGCCCTGCATGATTTTCACGACGAAATTGTGTCACG
GCAACCACGAGCTGCGCAAGACCATTCCCTGCTCGCAGCCCAACTTTAGCTGTGGCATGGCCTGTGGCAAGCCGTTGCCG
TGTGGCGGGCACAAGTGTATAAAGCCATGTCACGAGGGCTCCTGTCAGTCTGCCGGCGAGATATGCCGGCAGAGCTGCAC
CAAGCCACGACCGACCTGTGGTCATAAGTGTGCAGCTGCCTGTCACGAGGGAGCCTGTCCGGAAACGCCTTGCAAGGAGC
TGGTGGAGGTGCAGTGCGAGTGCGGCAACCGAAAGCAGAACCGCAGCTGCCAGGAACTAGCCAGGGAGCACAGTCGTATA
GCCACCATCCAGCTGGCCTCGTCCATGGCAGAGATGTCACGCGGCAATTACATGGAGCTCAGCGAAATCTTGGCTCCAGC
CAAGAAATCTAATAAAACTTTGGATTGCAACGACGAATGTCGTCTACTGGAGAGGAATCGTCGACTTGCCGCGGCCCTGT
CTTCTGGAAATTCCGATACAAAGCAAAAGTGTCTAACCAAGTACTCGGAGTTTGTTCGTGGCTTCGCAAAGAAGAACCCA
GCGCTGACCAAGAGCGTTTACGAGACGTTGACCGACCTGGTAAAGTTGGCCAAGGAAAGCAAGCAACGGTCGAGGAGCCA
CTCTTTCCCCACCATGAACCGCGAAAAGAGGCAGCTGGTGCACGAGTTGTGCGAGGTATTTGGGATTGAATCGGTTTCCT
ATGACAAGGAGCCAAATCGCAACGTTGTTGCCACGGCCCATAAGGATAGGTGCTGGTTCCCCGCCACGAGCATCATGGAA
GTGCTGGCTCGCGAGTCCGGACAACGCCGTGTCCCGGTGCCCAGTAACAATGCTTGGGGATTGAAGAAGTAGATCAAATC
CGTGTGGTATACAGAGATATTAAATATATTAAATCGTTGGCGCTGGAAAATAATAGTTAAAAATGGATAGATGATGCAAA
TGGAAGCCTGGATGTGGAAGACTTATTGATTGAACCTAAATGAGGAAAATTTTGTGGATGCGCTGCGAGGACAATACAGA
AGGATAATTGCCGTTACCACGTATATTTTGTAAATTATAACAAATGTTAGTTGAACCCTAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)