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FB2026_01
,
released March 12, 2026
CTACAATGGCATCGGAGGAAGAGAATGACGATAATTTCCAGGAAGAAGAAGAGGCCCAGGAGGACAACGCACCTGCGGCG GAGCTGTCCAACGATTCCGACGCCCCTCTGAAGCCGAATAACGACGAAGACGACGACTACGATCCGGAGGACAGTCGCAG GAAGAAGAAGGGCAAGAAGCGCAAGACCAGGAAGGGCGAAGAGAAGGGTCGCAAGAAGAAGAAGCGCAAGAAGAACGAGA GCGAAGAGGACAGCGACTTTGTCCAGCACGATGAGGAGGTGGAGTACCCCAGCACCTCCAAGCGCGGTCGCAAGCGCAAG GAGGAGAAACAGGCCGCCAAGGAGAAGGAGTCGGCATCATCCGGAATGCCATCTGTGGAAGACGTGTGCTCAGCCTTTAG CGTCTGCAATGTGGAGATCGAGTACAGTGAGGAGGAGCTGCAGAGCCTAACGACCTACAAGGCTTTCATGCATCATGTTC GTCCGATTCTGCAGAAGGAAAACCCCAAGATTGCGGCACCAAAGCTAGTGATGCTGGTGGCAGCCAAGTGGCGTGAGTTC TGCGAGAGCAATCCGCACATCCAGCAGGAGGGTGGTGCCGCCGGCTCCGGTGGATCCGCCGGACAGGCACGCAGTGTGAC AGGCGATGAGCCGGAGGAACCTCGATCATCGCGTTCCTCGCGCAACGAGAAACCGGATGACATTTACGAGGAGGCCGTCG AGGAGGAGGAAGAAGAGGAGGAGGAGGAGAAGAAACCTCGTCGTAAGCGCAGTGGACGTGGCAAGAAGGGGCGTCGTCCC TCCGGCAAGGTGCCCACTCTTAAGATTAAGCTGCTCGGCAAGCGCAAACGCGATAGTTCGGATGAGGAGCAAGATGCTAG CGGTGCATCGGAACGCGATTCGGATCTTGAGTTTGAACGGATGCTTCAGAAATCGGATGACAGTGCCGATGAAAAGGAGG CTCCTGTCTCCTCTAAGGCGGACAACTCAGCACCCGCTGCCCAGGACGATGGATCAGGAGCTCCAGTGGTGCGCAAGAAG GCCAAGACCAAGATCGGCAACAAGTTCAAGAAGAAGAATAAACTCAAGAAAACGAAGAACTTCCCGGAGGGCGAAGATGG CGAGCATGAGCACCAGGACTACTGCGAGGTGTGCCAGCAAGGAGGTGAGATCATCCTGTGCGACACCTGCCCTCGGGCAT ATCACTTGGTGTGCCTGGAGCCAGAACTCGATGAACCGCCAGAGGGCAAGTGGTCGTGTCCGCACTGCGAGGCTGACGGA GGTGCTGCTGAGGAAGAGGACGATGATGAGCACCAGGAATTCTGCCGCGTGTGCAAGGACGGTGGCGAGTTGCTGTGCTG CGACTCATGTCCCTCCGCCTATCACACATTCTGCTTGAATCCGCCTCTGGACACTATACCCGATGGCGATTGGCGCTGTC CTCGTTGCAGCTGCCCTCCGCTCACTGGCAAGGCTGAGAAGATCATCACCTGGCGATGGGCACAGCGTAGCAATGACGAC GGTCCCTCCACCTCGAAGGGATCAAAGAACAGTAACTCCCGGGTGCGCGAGTACTTTATCAAGTGGCACAACATGTCCTA CTGGCATTGCGAATGGGTGCCCGAAGTGCAACTGGACGTCCATCATCCGCTCATGATTCGTTCGTTCCAGCGCAAGTACG ACATGGAAGAACCGCCCAAGTTCGAGGAGTCGCTGGATGAGGCGGACACTCGTTACAAGCGTATTCAGCGACACAAGGAC AAGGTTGGCATGAAGGCAAACGATGATGCCGAAGTGCTGGAGGAGCGGTTCTACAAGAATGGCGTCAAGCCAGAGTGGCT CATTGTTCAGCGCGTAATCAACCATCGTACGGCAAGAGATGGCAGCACCATGTATCTGGTGAAGTGGCGCGAGTTGCCCT ATGACAAGTCCACCTGGGAAGAAGAGGGTGATGACATACAGGGCCTGCGGCAAGCCATCGATTACTATCAGGATCTGCGT GCGGTTTGCACCTCGGAGACAACTCAGTCGCGCAGCAAAAAGAGCAAGAAGGGTCGCAAGTCCAAGCTCAAGGTCGAGGA CGACGAGGATCGCCCGGTCAAGCACTACACACCGCCGCCGGAAAAGCCAACTACTGATCTGAAGAAGAAGTATGAGGATC AGCCGGCTTTCTTGGAGGGCACTGGCATGCAATTGCATCCCTACCAGATCGAGGGCATTAACTGGTTGCGCTACAGCTGG GGCCAGGGCATCGACACCATCCTGGCCGACGAGATGGGTCTGGGTAAGACCATTCAGACGGTCACCTTCCTGTACTCTTT GTACAAGGAGGGCCATTGCCGCGGACCCTTCCTCGTGGCCGTGCCACTCTCGACGCTAGTGAACTGGGAGCGTGAGTTTG AGCTGTGGTCTCCGGACTTTTACTGCATCACGTATATCGGTGACAAGGACTCGAGAGCGGTTATACGGGAGAACGAGCTG AGCTTCGAGGAGGGCGCCATTCGTGGTAGCAAGGTTTCGCGTTTGCGGACCACCCAGTATAAATTCAACGTACTGCTGAC TAGCTACGAGCTGATCTCCATGGACGCTGCTTGCCTTGGCAGCATTGACTGGGCCGTGCTCGTGGTGGACGAGGCCCATC GCTTGAAGAGCAACCAGAGCAAGTTCTTCCGTATCCTGAACAGCTACACTATTGCCTACAAGCTTCTGCTCACCGGCACA CCGCTGCAGAACAATCTCGAGGAGCTGTTCCATCTGCTCAACTTCTTGAGCCGCGACAAGTTCAATGATCTGCAGGCCTT CCAGGGCGAATTCGCTGACGTTTCGAAGGAGGAGCAGGTTAAGCGTCTGCACGAGATGCTTGGACCGCACATGCTGCGTC GTTTGAAGACGGATGTGCTGAAGAACATGCCGTCCAAGTCTGAGTTTATCGTGCGCGTCGAGCTGTCGGCCATGCAGAAA AAGTTCTACAAGTTCATCTTGACCAAGAACTACGAGGCTTTGAATTCGAAGAGCGGTGGTGGCTCTTGCTCACTGATCAA CATTATGATGGACCTAAAGAAGTGCTGCAACCATCCGTATCTCTTCCCCTCGGCCGCCGAGGAGGCAACCACCGCTGCCG GTGGTCTCTACGAGATTAACTCGCTGACCAAGGCTGCTGGCAAGCTGGTCCTGCTATCCAAGATGCTGAAGCAGCTGAAG GCACAAAACCATCGTGTCTTGATTTTCTCCCAGATGACCAAGATGTTGGACATT
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.