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FB2026_01
,
released March 12, 2026
TCGGATCTATACGTGCCCACCAAAAGGATATACAACCACACCCAACTAGAAGGACCTACTAGCCCAGCTGACATCCTGGT TGTCCCGCAAAACGCACGGCACATTCGAAAAGGATACGCGATCAGTGAACCCAAGTGAATAAGGTGCCGTCCAAAAACGA TACGATCCGAATTAGTGGCATACGAAATATCCTTGTATATTTGGGCCCTCATACAGACTGAGCCAGAACAAAGAAAAAAC GCCGAAATGCCGAGCAGGAAGAAAGCCCAGGGGGAATCGGCCGCTGGTGAGGATGCGGACATTGTGATCTCGGGACTTTC CGGGAAGCTGCCGGAGAGCAGCAACATCGAGGAGTTCAAGTACAACCTGCTCAACGGCATCGACATGGTCACAGATGAGC CGCGCCGTTGGGAGGCAGGCATCTATGGACTCCCCGAGCGTATGGCCAAAATGAAGGACTCCGATCTGGAGAAGTTCGAC GACAAGTTCTTTAGCGTCCACCAGAAGCAGGCGGAGCTGATGGACCCCTGCATGCGGATGCTGCTGGAGCTGACCCACGA GGCGATCATCGATGCGGGCATCAATCCCGTACAGCTGCGCGGCAGTCGGACGGGCGTCTACATAGGCCTGTCCTTTGTGG AGACGGAGCACGAGATCCCCAACATGGAGCCGAGCAGTATCAACGGCTACTGCCTGACGGGCTGTGCGCGTGCCATGTTC GCCAATCGCATCTCCTACACGTTCGACTTCAAGGGGCCCAGCTTTATCGTGGACACCGCATGCTCCAGCTCGCTGGTGGC CCTTAACCACGCCTTCGCGGACATGCGGGCGGGTCGCTGTGATTACGCCCTGGTTGCGGGCGTGAATCTCATCCTAAAGC CCATCTTTGCCCTCCAGTTCCTCAGGCTGGGCATCGTGAGCCACGACGGCTCCTGCAAGACCTTCGATGCGGCGGCCAAC GGCTATGCCCGGGCGGACACCTGCGCCGTTGTGCTGCTCCAGAGGCGTAAGGAGGCCAAGCGGGTGTACGCCAGCATCCT AAATGTTCGGACCAATACGGACGGATTCAAGGAGCAGGGCGTAACCTTCCCAGACGGCCGCATGCAGCAGGCCCTGCTGG AGGAGACCTACAGCGAGATCGGCCTCAATCCGGACGAGGTGGTCTACGTGGAGGCACACGGCAGCGGCACACCGGTGGGC GATGACCAGGAAGCCAACATGCTGAGCAACTTCTTCTGTCGCCCCTCGCGATCCACTCCGCTGCTTATTGGCTCAGTGAA GTCGAACATGGGTCACGCGGAGCCAGCCTCGGGAGTGAGTGCCCTGGCCAAGATGATCATCGCCATGGAGGAGGGCATTA TTCCGAAGAATCTGCACTATCGCACGCCCAATCCTTCGGTTCCGGCTCTGGTCGAGGGTAAGCTTAAGGTGGTGGATCGC AATCTTCCCTGGCAGGGTGGCATTGTGGGTCTGAACTCCTTCGGCTTTGGAGGAGCCAATGCCCATGTGGTACTAAAGTC GCACACTAAAGCCAAAGAGCCTAAGGCATGCCCGAAGGCTGGGGAGGCCGAACAGTCCCTGAGTCTAGTTACCTGTTCTG GCCGCACGGAGGATGCAGTGAATCAGTTGCTGAAAGTTGCCACCGAGCAGAGGCACGATCAGGAGCTGCTGACCCTCATC AATGACATCCACTCACATCCTATTCCCCTGCATCCGTTCAGGGGATACGCTGTTCTCGGTACCAGCGGATGCGTTAAGGA GGAGGTGCTGCCCTACGAGGAGGAGGAGCGACCCATCTGGTTCGTCTACGCCGGCATGGGCAGTCAATGGGCCAGGATGG CTAAGGATCTCATGCAGCTGGAGGTTTTCCGGAACTCCATACAGCACTGCGCCGAGGTTCTGGCCCAGGTGGACTTTGAT CTTATAGACGTGCTTACCCGTTCCACGGAGCGAACCTTTGACAATATGCTCTACTCCTTTGTCTCCGTGTCCGCCGTGCA GGTGGCGTTGACAGATCTTCTCCGGGTGCTGGACATACGACCAGATGGAATCATTGGCCATTCGGCTGGTGAGCTGGGGG CAGCCTACATGGACGGATGTCTAACGGCGGAGCAAACAGTTCTGGCCGCCTATTGGCGCGGCAGGAGCGTCCTGGACACT CCGGACTTGCCGCGCGGAAAGATGGCTGCTGTAGGTTTGAGCTGGGAGCAGATCGGATCACAGATCCCAAAGGATTGCTA TCCGGTGTGTCACAACAGTGATGACAATTGCACGGTCTCCGGGCCACCGTCATCCATGGACGCGATGATAGAGGACCTCA CGGCAAAGGGAATTTTCGTGAGAGAGGTTGGCTCTGGAGGCTATGCTTTCCACAGTCCGTACATTGAAGGAGCGGCTCCC ATGCTGCGTAGAAGTCTGGAGCGACTGATCACGGAGCCCAAGAAGAAGAGTATTCGATGGCTTAGTACTAGTGTGAAAGA AGCGGAGTGGGACTCGGAGAAAAACCACCTGGCATCGGCTGGCTACTTTATAAACAACCTCATATCCCCCGTGCTCTTTC ATCAAGCTGTCAAGCGGATTCCCCAAAACGCTCTCATCGTGGAGATCGCACCCCATGGACTCTTCAGGGCGATTCTCCGA TCCTTAAGTCCGCAGATTAGCTATGTGAGTCTGATGCAGCGAGGTCATGCCAATAACTTTGAGTTCTTGCTGAGCCAGTT GGGCAGACTCTACGCTGCCGGAGGGCAGCCGCAGATCCTGAAGATCTCCCCTTCCATCCCCTATCCCGTTTCCCGTGGCA CTCCCATGCTGGGCTCCTTAGTGGGCTGGGATCACACACAGAAGTGGAACTACCCCAAGTTCAAGGGCGGCAGACAGGCG GGACAGCTTAGTGTGGAGCTAGATTTGGGCAAGGAGGAAAACGTCTATCTAGCGGGGCACACGATCGACGGCAGAGTCTT GTTCCCCGCAACCGGCTACATGACCCTGGCCTGGATGAGTCTGGCCCAACAGCAGGGATTGGACTACTTGCGCACGCCCG TGCTATTCGAGGATGTGGTCTTCCATCGGGCCACGATCCTTAGCGTGGGCACTGTGGTAAAACTAACACTCAACTTCTTT CCGGGCAGCAGCTCCTTTGAAATCTGTGAAGGCACAAGCCTGGTGACATCGGGCAAGATCCGTCTGGTGAGCAATGTGCA GCAGGAGCAGCTTCAGCTGTCATCTCTACCGGGAATCGCCGGCTCCAACAAGCTGAGCACCAAGGACATTTACAAGGAGC TAAGACTTAGGGGCTACGACTACACTGGTGTTTTCCAGGGCATTTTGGAGTCGGATATTTCGGCGGTGACGGGTCAGCTG CAGTGGGCTGACAATTGGATCAGTTTTATGGACACCATGCTGCAGTTTCGCATCCTGAGCAATGATATCCGAGAACTGTA CGTGCCCACGGGAATAGAGAGGGCACTGATAGATCCTCTCAAGCACCTGGAGCTGGCAAAGAAGCACCAACAGAAGTTAA CTGTCAACTGGCATCGCAACATTTCCGTAGTCAAGTGCGGTGGAGTGGAGATACACAAGATGAAGACCGCCCAGACCCAA AGGCGTTCTGGTGGCCAATCTCCACCCAGCTTGGAGCGCTATAGATTCTGGCCTCATTCCCAGCACTTGGGTCAGCGGGA GGAGCAGGAAAAGTCAAGGAGCACAGCTCTAGCGGTGGCCATTCAATTGATCATCGAGAACAGCGGTGGAGCTGTAAAGC TCAAGGGCGTGGAACTGGCTGGATCCCGGTCCACTTTGGATGCGCTGAGTGCCGTCCAGCTTCTCGAGCTGATCGAAAGG GAACCGATTCTGGTGGGCGACATGGCGGTGGTTACCAGCTCCTCCAATGAATCCGAACTGGGTGAGCAGCTCAAGGAGCA CGGTATTCGGGTGATAGTCAAGGATATCAAAGCTGGTGCGGTGGAGCAGCAATGCCATTTCGTGTACTCTGTGAATATGC TAGCCCAGAACTCTGTCAAGGATCTGCAGCACTGCTTGGATAGTCTGAAGTCCGATTCGGGATTCCTGCTCCTGGAGGAA GCAGCCAGCAGGTATAACATTATAGGAAAGGAGAAGTTGAACAAACTTAAGCTGGTTTCAGTTTTGGAGCAGTTCTTCGG TACAGATCGAGTGCTTGTCCTAGTCAGAAAACTATCGAAGGTGCAATCCAGCCAAGCCCTGACACTTCACCTCACCAATA AACAGTTCAAGTGGGTCAACGAGCTAAAAAACTCTATTGCAAAGGCCCAAGCTGAAGAGAAAAACCTCTACTTGGTGGCC CAGGGTGAACCCAATTCAGGAGCTTTGGGCTTGATGAACTGCCTGAAGAGGGAAGCTGGAGGGCATTTCCTACGGCTATA CCTTATTCTGGATGAAGGTGTGCCCCAGTTCTCCCTGGACGATCCCTTCTACGCCGCACAGTTGGCCAAGGATCTGGTCG TCAATGTCTACAGAAATGGAAGCTGGGGTAGCTACCGTCACCTGAAGATGGAATCCCGGCCGCCCATGCTGCCAGTGGAG CAGGCGTATGTGAACACCCTGGTCAAGGGGGATCTCTCATCACTTCGCTGGATCGAGAGTCCTCGTTCCAGTCCCATCCA GTCGCAATTGGAGCCCTGCACCGTTTACTATGCTCCGCTCAATTTCCGGGATGTGATGTTGGCCTCCGGTAAACTGGGAG TGGATGCTCTGCCTGGGGATCTGGCCTACCAGGACTGTGTCCTTGGTCTGGAATTCGCTGGCAGGGATTCGTGCGGCCGT CGTGTAATGGCAATGGTTACTGCCAAATCTTTGGCCACCAACTGCTTGGCCAACAGGAATCTCCTCTGGGAAGTCCCATC TAAGTGGACCATGGAGCAGGCTTCGACCGTTCCCTGTGTTTATGCCACCGTTTATTACGCTTTGGTGGTCAGGGGCCAAA TGAAGGAGGGTGAAAGGATCCTCATCCACGCCGGCTCTGGAGGAGTTGGTCAGGCGGCCATTTCGGTGGCTTTGGCTCAC GGACTGACTGTCTTCACCACTGTGGGCAGCAAGGAGAAGCGGGAGTTCCTGCTGAAGCGATTCCCCAAGCTGAAAGCTAA GAATATTGGCAATTCGCGAGACACCTCTTTCGAGCAATTGATCATGAGCGAAACCCACGGAAATGGAGTGGAACTTGTGC TGAACTCTCTCTCCGAGGAGAAGCTGCAAGCCTCCATTCGCTGTCTGGCTTTGAATGGCCGCTTCCTGGAGATCGGCAAG TTTGACTTGAGCAACAACACTCCGCTAGGGATGTCGGTGTTCCTCAAGAACACCTCCTTCCACGGAATCCTTCTGGACAG CGTGATGGAGGGGGAGGAGGAGATGCAGCTAATGGTTGCCAAGTTGGTGGCGGAGGGTATCAAGAGTGGAGCAGTGCAGC CGCTGCCTACCACAGTCTTTGCAGAGCAGGAGATCGAGAAGGCGTTCCGTTTCATGGCGTCCGGCAAACATATTGGCAAG GTGGTGATCAAGGTACGTTTGGAGGAGGAACAGCAGGCGGCTCTCCCGCGACTTAGGCAAATTCAGGCCATACCGCGCTC CTACATGCACCCAGAGAAGAGCTACATCTTGGTGGGGGGACTTGGGGGATTCGGTCTGGAGCTGGCCAACTGGCTAGTTT CCAGAGGAGCCCGCTTTCTGGTTCTGAGCTCTCGCTCTGGGGTGAAGAGCGGGTACCAGGCCCTGATGATTCGTCGCTGG CACGACCGTGGAGTTCAGGTTCAGATCGATACCAACGATGTGACCACCGCTGTTGGCTGCCAGAGACTCCTAGAGGTTAG TAATAAGCTGGCCCTGGTTGGAGGTATCTTTAACCTCGCGGCTGTACTACGGGATGGACTGATCGAAGATCAAAGCCCGA AGAACTTTGAAAAGGTAAGTGCACCGAAGCTACTGGCCACCATACACTTGGACAAGATCTCCCGGGAAATTTGTCCTGCA CTGGAGTACTTCGTGTGCTTCTCCAGCTTGTCCTGCGGCAGGGGAAACATGGGACAGACCAACTATGGACTGGCGAACTC CACGATGGAAAGGATATGCGAGCAGCGGCAGGAATATGGTTATCCGGGCACAGCGATTCAATGGGGAGCAATTGGGGACA CCGGACTGATCATCGAGCACATGGGCAACAACGAAACAGTTGTGGGCGGCACTCTGCCGCAGCGGATGATCAGCTGTCTG GAGACTCTGGACCTATTTCTGCAGCAGCCCCATCCCGTCATGGCCTCCATGGTGGTGGCGGAAAAACGCAAGACGGAGCA GGCCAACCGGCTGAGCTTGATAGCCACGATCGCGAATATCATGGGACTGAGGGATGTAAAGAGTGTCTCCGATAAGACCA CCCTCTTCGATCTTGGCATGGACTCGCTGATGAGCACAGAGATCAAGCAGACGTTGGAGCGTCACTTTGACCTGGTGCTG AGCGCCCAGGAGATCCGCCAGCTAACCTTTAGCGCCCTGCGCCAGATAGACGCCCAGGAACCGCCCTCCGCTTCAGTCTC ATCGCCCTCAGCCTCTTCCACCCTATCAAGGATCAAGGAGGAGGCCTGCGTGGAGGTCCAGCCCCAGAGCGATGAGACGC GCAAGGTATTCGCCAAGGAAGTATTACCCCAGGAGGTGATGGTGCGCCTTCGGTCAAAGGCGCCCGTGGCCAGCGAGGAT CCGGCTGTGTTCTTCTTGGCTCCCATCGAGGGATTCACAATAGCCGTGGAGGCGCTGGCCGCCTCGCTCACCTGCCCCGC CTACGGGCTGCAGTGCACGGAGCAAGTGCCCCTGGACTCCATCGAGGCCTGTGCCGCCTACTATCTGCGCCAGATCCAGA AGCTCCAGCCCCTCGGACCGTACCACATCGTAGGCTACTCCTACGGCTGCCTGCTCGCCCACGCCATTGCTGTGGCGCTG GAGCAGCGCCGCTTCGGGGTCAAGGTCATCATGCTGGACGGAGCGCCCACCATGGCCAGCGGCTATGTCCAGGAGGCCAA GAAGCAGACGGACGACCTCAACCGGCAGCAGTCCATGACGTTGGCCTACTTTGGAGCTCTGCTCGCCGACGTGGATTACA ACCAGCTACTGCAACTCCTCGATGGAGTCCAGACATGGCCCTCCAAGCTCGACAAACTAGCAGACACTCTGGCTGGTTAC ACGCAGCAAACCAGAGAAGTGATCAAGAAGGCCGCCTGCATGTTCAAACAGAAGCTGCTACTTTCTGAGAGCTACAAGGG CGCCAAGCAGCTCCACAGCGATGTGGTCTTGATCAAGTCGGCCGAGCACAATGCGATTATGGCCCAGGACTATGGCCTCA AGGAGATTTGCAGTGCCCAGATCGACATGCATGTGGTGGAGGGTACACACAGGACCTTCCTCAAGGAGCCGCACACTCTA CAGATCATCGAGCGCGTCCTGCGGAAGCGTCCCTAGTGGAAGGGAAGTAGTTCGTACGATTATGCTGATGAATTATCCTG TAGAACTTAATGTACATATACCCTAATCCTATCAATACACGTAGCAATCGCGCATTGATTGTTTAAGAATAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.