FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH10726
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General Information
Symbol
Dmel\GH10726
Species
D. melanogaster
Name
GH10726
FlyBase ID
FBcl0112413
Feature type
cDNA_clone
Computed gene(s)
Dmel\kkv
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,482
      NCBI
      AY051434
      Full Length sequence dataSequence Downloader
      CTCGATCCGGACTATTATGAATTTGAGACCCACATCTTCTTCGATGACGCCTTCGAAATCTCCGATCACAGCGACGATGA
TATCCAGTGCAATCGATTCGTCAAGCTCTTGATAGCCACCATGGACGAGGCTGCCTCCGAGATCCATCAGACCACGATCA
GACTGCGTCCGCCCAAGAAGTATCCCACTCCGTACGGAGGCCGCCTGGTGTGGACCCTTCCAGGGAAGACCAAATTCATT
ACGCACTTAAAGGACAAGGACCGCATTCGTCACAGGAAGCGTTGGTCTCAGGTCATGTACATGTATTACCTGCTGGGACA
TCGTCTAATGGAGCTGCCCATTTCGGTGGATCGCAAGGATGCAATTGCGGAGAACACCTACCTTTTGACCCTGGACGGAG
ATATTGACTTCAAGCCGAATGCGGTGACTCTACTGGTGGATCTGATGAAGAAGAACAAGAACCTGGGTGCTGCCTGCGGT
CGCATTCATCCCGTGGGATCGGGACCCATGGTGTGGTACCAACTTTTCGAATACGCCATCGGCCATTGGCTGCAAAAGGC
CACGGAGCACATGATTGGCTGCGTGCTCTGTTCACCTGGATGCTTCTCCCTGTTCAGGGGCAAGGCCCTCATGGATGACA
ATGTGATGAAGAAATACACGACACGGTCGGATGAGGCTCGTCACTACGTGCAGTACGATCAGGGCGAGGATCGTTGGCTG
TGCACATTGCTCCTCCAGAGGGGATACCGCGTGGAGTACTCGGCTGCCAGTGATGCGTACACCCACTGCCCCGAGGGATT
CAATGAGTTCTACAACCAGCGGCGCAGATGGGTGCCCTCGACCATAGCCAACATCATGGATCTTCTGGCGGATGCGAAGC
GCACGATCAAGATCAATGACAACATTTCGCTTCTGTACATCTTCTACCAGATGATGTTGATGGGCGGCACCATCCTGGGA
CCCGGAACCATTTTCCTTATGTTGGTGGGTGCATTCGTGGCAGCCTTTCGCATTGACAACTGGACCTCCTTCCACTACAA
CATCGTGCCGATCCTGGCCTTCATGTTTATCTGCTTCACCTGTAAGTCGAACATCCAGTTGTTCGTGGCCCAAGTCCTTT
CGACGGCATATGCCCTGATCATGATGGCGGTGATTGTGGGTACGGCCTTGCAGTTGGGAGAGGACGGAATAGGCTCCCCT
TCGGCCATTTTCCTGATATCCATGGTGGGCTCATTCTTCATAGCCGCATGTTTGCATCCACAAGAGTTCTGGTGCATTAC
ATGCGGCCTAATCTATTTGCTGTCCATCCCGTCCATGTACCTGCTGCTCATCTTGTACTCTATCATCAACCTAAACGTCG
TCTCCTGGGGCACCCGCGAGGTGGTGGCTAAGAAGACCAAGAAAGAACTGGAGGCGGAGAAGAAGGCCGCCGAGGAGGCC
AAAAAGAGGGTGAAGCAGAAGAGCATGCTGAGCTTCCTTCAGAGTGGAATCGGGGACAATGGCGACGAGGAGGGCTCCGT
GGAGTTCTCTCTCGCTGGACTCTTCCGCTGCATATTCTGCACCCACGGAAAAACATCCGACGAGAAGCAGCAGCTGACCT
CCATCGCCGAATCGCTGGACACGATCAAACATCGAATGGACACCATCGAGAGTGCTGTGGATCCCCATGGTCACCATGCC
TCCCGACATGGCAGGAGGAGAACCACATCCAGTGGCTCCAAGGATCACCACCTGCTGACCTCGGTGGCGGAGAAGTCTGG
CGATGAATCGGACGAGTCGGACTCAGACACTTCGGCGGAACCAAAGCAGGAGCGAGACTTTCTAACCAATCCCTACTGGA
TCGAGGACCCCGATGTACGCAAGGGCGAGGTCGATTTTCTCTCCAGCACCGAGATCCAGTTCTGGAAGGATCTCATCGAC
CAGTATCTCTATCCCATCGACAATGATCCCGTGGAGCAGGCCCGCATAGCCAAAGATCTGAAGGAGCTGCGCGACTCGTC
GGTGTTCGCGTTCTTCATGATCAATGCACTGTTCGTGCTGATCGTGTTCCTGCTGCAGCTGAACAAGGACAACATCCACG
TGAAGTGGCCCTTCGGAGTGCGGACGAACATCACCTACGACGAGTCCACCCAGGAGGTGCACATCTCCAAGGAGTACCTT
CAGCTGGAGCCCATCGGATTGGTGTTCGTATTCTTCTTTGCTCTTATTTTGATAATCCAGTTCACGGCCATGCTGTTCCA
TCGATTTGGAACCATTTCGCACATCCTGGCCTCGACTGAACTCAACTTCTGCAAGAAGAAGTCTGAGGATCTGACACAGG
ACCAACTAATAGATAAGCATGCTGTGGAGATCGTCAAGAACTTGCAGAGGCTGCAGGGAATCGATGGCGACTACGACAAT
GACTCCGGTTCCGGGCCAGATCGCATAGCCAGGCGGAAGACCATTCAAAATCTGGAGAAGGCGCGACAGCCACGTCGCCA
GATCGGCACCCTGGATGTGGCGTTCAAGAAGCGCTTCCTGAAACTCACCGCCGATGCGGAGAACAATCCGGCCACGCCCA
TTCTCACCCGCCGCCTGACAATGCGGGCGGAGACCATCCGGGCACTGGAGGTGCGTAAGAATTCGGTGATGGCCGAAAGG
CGGAAGTCCGCGATGCAAACTCTGGGAGCAAAGAACGAGTACGGAATCACAACTGGAGCACCGATTAACAACAATGGAGC
TCTGCCGAATCAGCGAAGCGGACGTGTCTCGAATGCAGGCATCAGCATCAAGGATGTATTCAATGTGAACGGTGGTGGTG
CCGAGCAGATTTACGGCTCGAATGGGGGCGGCACTATCAACCAGGGTTACGAGCATGTGATCGACGAGGACGGCGACGGC
AACTCCCTGCGGCTGACCACCAGGAATCCCCATCCCCACCCGCACCACCAGGTCTCCTGGGGCCAGAACACCAACGGCGG
CGGCGGTAATGGCACAGGTCGCCTGTGAAGGACCCGCCCATCCTAGACGTAGAGCTAAGTTAGACCTAGAATTGTAGTCA
GAAGGAAGGATTGTGTTGTATAGTATGTATAGGGACGCGAGTGAGAGCCAGGACACTCTGGCTCAGAAGGATCAGGTCGT
TCATAAGGCTTCATGGCAACAGAACGAACATTATGCTTAGCTAATCACACTGCCAGTGTCGATGTGTGCCAATAATTGGG
TGGCTGATCGCCCACTGTGGCAGCAACATTCATGCGATGTACTATACTATCATTTGTTAGGTTATCTTTAAGTCAATACC
CGCGTGGATCATCCGAAACACGCGGCCAGTGCATGCTGACACAAAATACTCAACGTGGAGCTGAACCGCAGTGGCCTATT
TGTACACTCGACGACATATCGAGGATAGTTCTTTTTAAATGAATAATCATTGATGATTTGATGGATTGTAAAACTTTTTA
AAACAATAAATAGAAGCATCAAACAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)