FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH13327
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General Information
Symbol
Dmel\GH13327
Species
D. melanogaster
Name
GH13327
FlyBase ID
FBcl0113401
Feature type
cDNA_clone
Computed gene(s)
Dmel\spir
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI03875
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,431
    NCBI
    AY089503
    Full Length sequence dataSequence Downloader
    AGACGCCTCGTCGTTGTCCGCTCTGCAGCTAACAATTATTCTGTGGGCTAAGTGAAAATGAAATCGAAAGCGGTGGCTAT
CTAGTAAAATAAGCAAAGTACAACAAAGTGACGTGTGGATATTGCGAAACCCTCAACCATATTTAATGGAGCTGCTGGGC
AAACCCGACTGATTCCCATCAACCCTCCCTGAAAAGAGAGAACAGTGCGGCCCAGAGGGTGGCTTCCCTTTTTCGGACTC
GGATCGATAGCCTCCATGCAACCGTGTGAGAAAACCGAAAAGTGAAAAGCCAAACGGAAATGTGCCAGGCTTAACCCATG
GAAGCCCGGCCAGCGAAACGCTCCACAGCGGCCTCCGTCTTCCGGTCGCATCACATGCCGCGGGAGTCGGATCTGGTGGA
CATCGGCAGCGATGCCTCGCTGTACTGCGGCTCCGACGGGGAGTCCAGTCAGGCCCAGTCCACATCCACATCCACTCCGA
ACCCGCAAACGAGTTCCGACCAGGATCTGGACCAGCCTCAGCCAACACCTCGCGCGGCACCGAGAGCATCGGCCTCCAAT
AATCCGCCAACACCCAAGCCACGACAGGCTATCAGGTCATCAAAAGTGCTTCCAAGTGCAAACTCCACGCTGTCACGATC
CAGACAGCGGCTCATCAAAGTGGACTTCTCCAAGTTTCAGGACGACGATCTCTTCTACGATGAGAACAGCATCAGCAGCT
CCCACTCCACAGCGGCCACCCACCAGCATCATCCACATTTCGCCGAGATGCACCGCTGCTCGCAGCCCAAGATGCCGCCG
TATCCATTCGGTGGCTACATGGTGCCCAGTCAGGCACGACAAGATTGCCGGGAGACGGCGTCGCTGATGCGGCCCCGACG
CACAATGGAGCCAGCAAAGCAGGTGCCGCCTCCAGAAGAGCCATCCTTCACGAAGGACGAGTACCACAAGTTCTACGACA
CGGCCCTGGAATCCTACGACCTGGCCACTCAGTGCGAGTCGAGGAGGGCTTCCTTGCGCCGCCACACCATCGTTGGATGC
CAGAGCAACCTGGACGAGACGCACTCAATGCCGCCCACGCGGCCCGAGTCGCGTCAATCGGACGACGTCAGCAAGGAGAC
GCCTAAGAGAAGTCCTGCGGAGCAGACCCATCCCTCAGACGAGGGCAGTTCAACATCCTCGCTTGGGCCATGGAACAAGT
CCTTCATGGACAAACAGACGTGGATGGAACGGGGTGACGATCGGCTGTCCGTAACGCTGGCGGAGATCGTTCACATCCGT
TCCGTCATGACCAAGGCGGAGCTGGAGGGCCTGCCGATGGACGTGCGCGTCAGGGAGGATGTTGAAAAGCGTCGCGTCTG
CTTCCTGTGCCTGCGCACTCGGTTCTCGTTCTTCGGTCCCTGGGGCATTCAGTGCAAGTTGTGCCAGCGCACGGTCTGCG
CCAAGTGCTATACCAAGATGCGAATTCCCTCGGAGCACTTCCGAAACGTGCCCCTCGTGCTGATCTCGCCATCGCTGCTG
TCCAGTCCGGCCAGCTCGAGCACCCCATCACCCTCCCATCATGCCCAGCAGGCGCACTCATCCTCGACGGGGAACATCAT
GGACGACCAGTTCCCCAAGTCGTTGATAGAGCGTCTTTTGCGCTCCGAGTCAGATCGAAAGACTCGCAGCACTGTGGGCA
GTGCCCCATCCTCACCCAAGCACCAAAGATCAAACATGAGCACGCCCGGCATTAGCGTGGGACCCGGAGCATCCTCCTCC
TCAGCGGCCGCCACTGGACAGGCCGTGGAGGCACTGCACGACCAGGCGACCATGTCGTCCTCGTATTCCGCGGCCATGCG
ACCCTCTGGCGTCCACCAGCAGCAGAAGCAGCACTACAACAACGCCATGTCGAGGAGCATGGAGGGACCCCGAAGTCTGC
CCGTGCACAGTCCAGCATATCGACCGCTCTCCAATAACAGCACCTTGGAGAGGAAATCCCGCTTTTCGAGGGGATTCAAC
CTGTTCTCCTCGGGCAGCCATCTTGCCCAGACGCAGGAGCAAAAGGAGAACCTGCGCGGCGAGCAGGTGACCGTGTGCAA
CGACTGCCAGGGACTGGTCAACGAGATCACGAGCTCCGTGAAGCAGAAGCGCAGCTCCGCACGGAACCGCACCATTCAGA
ATCTCACCCTGGATCTGACGCCCGTCTGGAAGTAGGCGGGAATCAGATCAGAGATAGGATAACTCCGCAGTGCGTTTGAA
GACCGTAGGATGGTTGCAATGGAGTTCTAACGACTGACTTGGCTCTACATCGCTATACATACTACCATTGACTACATGAC
ACTGATGATGACTCATTGACAGACAACAGACGCTATTTGATTACTGACCACATTCCTTACCTGTGAGAATTTTCAGTTTC
AGCGCGGCTGTTTTAGGGCCTTCAGAACGCACTTGCACATTTAGCTATTACATACATGCATAGATATGGATTAGATCGAC
TGTTGTACACTTAAAAGGATAGAGCCTCGAGGAGCCTCGTTTGTAAATCGTGTTAGTCTAGTGAATAAGCAGAGGAGAAT
ACCGAGAATTCCTGGCTGAAGCTTTGTACATTTTCTGCCGGCTACAGAGAGATCCGCTTGAGCGCACCCCACCACTTTCT
ATTTATAAAGGAGCTAACTTACACGTCGCTACACATTATATTATCGCAGATCGCAACGCGATGCCGTTGTTTATAACTGT
TCAAAGTATGCGTAAAAGTTGATTAAGTACATGTAATCATAACTCTTGTATCCGCATATATCAAATGCACATATATTCGA
CCTTACTTGTACCCTGCTCCTGCATTCGTTTCAGCACGAGCGACGTATTGATCATACAGAATACATCGATATTCGCAATA
CGATTCCCAGTTCAGAAAAAAAAAATTTCGATTGTTGAAATAGTTATCCGAAAATGTTGTGAAACTTTTGTGCTTGCGCA
GATCTTTGTTTACGTTGTGAAGTTTTCGTTTTGTTCCCCTATTAACTACAATGTCAATGTATAACGCAAGAACTACTTGA
TAGCCTAATTTAATTTTAGTCGGCGTTGCTATGCAGCCCAAACTATTTAGTTAATTCGCTTAGTTTTAATTCTCCCTAAG
TGCGTCTAAGTTGTAAGCGACGTCTATATAATTGTACAAGCTGATGATGCAAAGCAAACCCCTAACGAACATTATATTAC
CTAATTGTAGCTGCTATTATAGTTTTTCTAACCGTTTTTGAACAATTTTGTTTGATTTATCTGTAATTGTAAATGCATTT
AAGTACTTGTAATATTAAATTTTATGCTTTTAAGTTAAATTCATTTACAATAATTTAATACACACACGAACACACAAAGA
GATCGAAAATACACAAAACAGCAATAATAAATGAAAATACAAACTAAAGAACAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)