FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\GH01554
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General Information
Symbol
Dmel\GH01554
Species
D. melanogaster
Name
GH01554
FlyBase ID
FBcl0120517
Feature type
cDNA_clone
Computed gene(s)
Dmel\PGRP-LE
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,374
      NCBI
      AY058258
      Full Length sequence dataSequence Downloader
      TGGACTGATATGCAAAAAAGCATTTCACCACGGCACCTGCGCATATAATGGTGGATAGCCTGTGGAACGTCCTTATCTTA
TCGTGTAAGGTGGACACGACACGAACACTAATCAGAGAATAGAGCAGTTCTAACTCACAATATTGATAAACAAAGTAAGG
GCCAGCCGAGAGATACACGCGCATTTATTGGCAGCAAACAGAAGCCAAAACTACGGACATGTCCGAATCGGGAATCAAAA
AGTTGAGCCAGGAGCGGACTCGCGAATGGTTGGCTAGTCAGGAGGACGAGGAACTGGAGTCCATTGCAGAGTCCTCGGTT
GTGGACAGCTTGGACTACGATTATACCGAGGAAGAGGAGGATGCCGACCAAAATACCAGTGAAGAAATCAGCACTATGAC
ACTAGGCACTCAAATCGCTACCAAAAAGCATTCGATCATCAGCGACACCATAAGGGACCTTATGAACTCGATCAACAGCA
TTCAGACTTTGGGCAACGTTAATATAAGCAACTCCACGAACGTCCATATCGGCAATGTTACCAATATTAATGGAAATATA
CAAATCATAGCCGATGGCCTTACTCAAAACCGAAGAGATCGGCGGCATGTTTCACCACCGAGAGATAACGCTCCCAAAAC
TCCGACACATTTCGAAGACGACTATCAGGACGAGTCGGAGGAGAGGGTGCGCTCGGATGTGTTCATCAGGCGCCAGAAAT
TCAAAATACCCAAGGAGCTGTCCGCCATCATTCCGCGCTCTTCGTGGCTAGCACAGAAGCCCATGGACGAGCCACTGCCC
TTGCAACTGCCTGTTAAATATGTGGTTATCTTACACACAGCTACAGAGAGTTCAGAAAAGCGGGCGATCAACGTAAGACT
TATCCGTGACATGCAGTGCTTCCACATTGAGTCGCGGGGATGGAATGACATTGCGTACAACTTCCTGGTCGGCTGCGATG
GCAACATCTACGAGGGACGCGGATGGAAAACGGTGGGCGCCCACACTCTGGGTTACAACAGGATCTCGCTGGGGATCAGT
TTCATTGGATGCTTCATGAAAGAACTCCCGACGGCAGACGCCTTGAATATGTGCCGCAATCTGTTGGCCCGCGGCGTTGA
GGATGGTCACATATCCACCGACTATAGATTAATTTGTCACTGCCAGTGCAATTCGACGGAGAGTCCTGGTCGGCGATTGT
ACGAGGAGATACAGACATGGCCCCACTTTTACAATATCGAGGAGGAGGAACAATGAGGGACAAAAGAAGAGCACCATTTC
ACATACTTAGACTTAAATGCACCCGAGTTACGTTTATAATGTTGAACATTAAATCACCTGTTTATAAATTTTCCAAAAAA
AAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)