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FB2026_02
,
released June 18, 2026
CGCACGTGTGCTCCGCTCAGTGTTAGTGTTATTGTGCTTCAATTTGGGCCCCACGACATGGTCATCCTTCGCTACTACGA TGTGCAGGCCCACTCCGCGGCCGAGGAGGAGAGTGTCCTGCGTCGGTTGCGCGAGGAAGACGGCGCCGTGGTGTCCGTGC GCATGGAGCGCTGCTATCATCTGGAGTACAGCGCTCAGGCAGAGCACTCACTGGCCCTGGACGAGCTGCTGGTGTGGCTG GTCAAGCAACCGCTGAGCAAAGGCCAGAGCTTGTCCAGGCAACCTGCCCTGCAGTCAACTGGGTCGAGTCAGTTGCTCCT GGAGATCGGGCCGCGTTTTAACTTCTCCACGCCGTACTCCACGAACTGCGTGAACATATTCCAGAATCTCGGGTACTCAG AGGTGCGTCGCATGGAAACCTCCACCCGCTATCTGGTTACTTTTGGCGAGGGATCAAAGGCGCCGGAGGCAGCCAGGTTT GTTCCTCTGCTCGGTGACCGCATGACCCAGTGCTTGTACACCGAGGAGAATACCCCCAAGGCGAGCTTTGACGAGCAGCT ACCTGAGCGCCAGGCCAACTGGCATTTCGTGCCCGTTTTGGAGGAGGGTAGGGCGGCACTGGAGCGGATTAATCAGGAGC TGGGCTTAGCCTTCAACGACTACGATTTGGACTACTACCACGACTTGTTTGCCAAGGAGCTGGGCCGCAATCCCACCACT GTGGAGCTCTTCGATTGCGCCCAGAGCAACAGTGAGCACTCGCGCCACTGGTTTTTCCGCGGACGTATGGTGATCGACGG CGTGGAGCAGCCCAAGTCGCTGATTCGCATGATCATGGACACGCAGGCCCACACGAACCCGAACAACACCATTAAGTTCA GCGACAACAGCAGTGCCATGGTGGGATTCGATCACCAGACCATAGTTCCGTCCTCCGTAGTCGCTCCCGGCGCAGTGCGT CTGCAGAGCGTGCAGTCTGACCTGATTTTCACGGCGGAGACCCACAACATGCCCACTGCAGTGGCGCCTTTCAGCGGAGC CACCACCGGCACTGGCGGACGACTGCGTGATGTCCAGGGCGTGGGCAGAGGAGGCGTGCCGATCGCCGGCACCGCTGGCT ACTGTGTTGGCGCTCTTCACATTCCAGGTTACAAACAGCCGTACGAGCCTTTGGACTTTAAATACCCTGCGACGTTTGCG CCCCCACTTCAGGTGCTCATTGAGGCGAGCAATGGCGCCTCCGACTACGGAAATAAGTTCGGCGAGCCAGTGATCTCTGG TTTTGCCCTCTCCTATGGACTGAACAGTGCTGCCGATGCCAGCCAGCGGGATGAGTACGTGAAACCGATCATGTTCAGCG GTGGCTTGGGCACCATGCCCGCTACCATGCGCGAGAAGCTGCCGCCGGCACGCGGTCAGTTGCTGGCCAAGATCGGGGGC CCAGTGTACAGGATTGGAGTGGGTGGCGGCGCCGCAAGCTCTGTGGAGATACAAGGATCCGGAGATGCTGAGCTGGATTT TAATGCCGTACAGCGAGGAGACGCCGAAATGGAGAATAAATTGAACCGTGTGGTGCGAGCCTGTCTAGACCTGGGCGAGC AAAACCCCATATTAGCCATCCATGACCAGGGGGCTGGCGGCAACGGCAATGTCCTCAAGGAGCTAGTGGAACCAGGCTTT GCCGGAGCTGTTATCTTCTCCAAGGAGTTCCAACTCGGCGATCCCACGATCACTGCCCTCGAACTGTGGGGTGCCGAGTA CCAGGAAAACAATGCCATTCTTTGCAACGCTGATCAACGCGAACTGCTGGAAAAGATCTGCCGCCGTGAACGCTGTCCCA TCAGCTTCGTGGGAGTGGTGACCGGAGATGGGCGAGTAACGCTGCTGGAGAAGCCTGCCCCCAAGGATCTGGAACAGGCG TTGAATGCGTCCAATCGCAGCGAAGTCTCACCGTTCGACTTGGAACTTAAGTACGTTTTGGGCGACATGCCCAAGAGAAC GTACGACTTGAAACGCGAACAGACGCCGCTCAAGGAGTTGAGTCTGCCGAAAGGTCTCCTTCTGGACGAGGCCTTGGAGA GAGTTCTTAGCTTGGTGGCAGTCGGTAGCAAGCGCTTCCTGACCAACAAGGTGGATCGCTGCGTCGGTGGATTGATTGCA CAGCAACAGTGCGTGGGACCGCTCCAAGCTCCACTAGCGGACTACGCGTTAACCACCGTCTCCCACTTTAGTCACTCTGG AATCGCCACTTCAATTGGTACACAGCCACTAAAAGGACTGCTTGATCCCGCCGCAATGGCCAGGATGTGTGTGGCTGAAG CGTTGAGTAACCTTGTGTTTGTAAAGATCAGCGTACTGGCTGATGTCAAGTGCTCTGGCAACTGGATGTGGGCTGCAAAG CTGCCCGGCGAGGGTGCCCGGATGTTCGACGCCTGCAAGGAGCTCTGTCAGATTCTTGAGGAACTTCACATCGCCATCGA TGGCGGAAAGGACTCCCTGTCCATGGCGGCTAAGGTGGGCGGTGAGACAATAAAGTCCCCGGGTACTCTTGTCATCTCCA CCTATGCTCCCTGTCCGGATGTGCGGCTAAAGGTAACGCCTGATCTTAAAGGACCCGGCGCGGGGTCGAAGACTTCCCTG CTCTGGATAAATCTGGAGAACAGCGCTCGCCTAGGTGGATCGGCTCTGGCTCAGGCATACGCGCAGCAGGGCAAAGATAC GCCAAACTTGACAAGGAGTGATGTGCTGGGCAAAGCATTTGCTGTGACTCAATCGTTGTTGGGAGAAGGACTTATTCAAG CAGGACACGATGTCAGCGACGGAGGATTGCTTGTTTGCGTATTGGAGATGGCCATCGGAGGACTGAGTGGCCTTAGAGTG GATCTATCGGAGCCCTTGGCCAAGCTCAAGAACTTCGATAAGTCTGTCGAGAAACTTAATCGTCCCGAACTAGCTGTTCT CTTCGCCGAGGAGTGTGGATGGGTGGTGGAAGTCCTGGACACAGACCTGGAACGAGTTCGATCTACCTACGAAAAGGCAG GAGTACCAAACTACTATTTGGGCGTGACCGAGGGATTCGGACTGGATTCCAGAGTGGTACTCAAGAACGGCAAGTCAGAG CTGCTGGACCAGCCACTCCGTGTGCTCTACAAGAAATGGGAGAGAACCAGTTACGAGCTGGAAAAGCTTCAGGCCAACCC TGAGTGCGCCGAGGCAGAGTACAATAGCCTTGAGTATCGCCAAGCACCTCAGTATAGGGGACCTCAGAATGTGCAGGCCG AGCTTACTCTCAAGCGATCCTCCGCCCCAGTTCGCGTGGCCGTGCTCCGTGAGGAGGGTGTCAATAGTGAGCGCGAGATG ATGGCCTGCCTGCTGAGGGCTAACTTCGAGGTGCACGATGTGACCATGTCGGATCTTCTTCAAGGCACCGCAAGCGTGTC CCAATACCGCGGACTAATTTTCCCGGGCGGCTTCAGCTATGCGGACACTTTGGGATCCGCCAAGGGCTGGGCAGCAAACA TCCTCCACAATCCCCGCTTGTTGCCTCAGTTTGAGGCTTTCAAGCGTCGTCAAGATGTGTTCTCCTTGGGCATTTGCAAT GGTTGCCAGCTGATGACACTTATAGGATTTGTTGGCAGTGCAAAGAGCGAGGTGGGCGCAGATCCCGACGTGGCCCTGCT CCATAACAAATCGCAGCGGTTCGAGTGTCGTTGGGCCACCGTGAAGATTCCAAGCAATCGCTCCATCATGCTCGGAAGTA TGAAGGATCTGGTTCTTGGCTGTTGGGTGGCTCACGGCGAAGGTCGCTTTGCATTCCGCGACGAGAAGCTCATTAGCCAC TTGCAGTCGGAACAGCTGGTGACATTGCAATATGTTGATGATGTAGGCAAACCGACGGAACTGTATCCACTTAACCCGAA CGGTAGTCCTCAGGGAATTGCTGGCCTTTGCTCGTCGGATGGACGTCACTTGGCCCTCATGCCACATCCCGAGCGATGCT CCTCCATGTACCAGTGGCCCTATGTGCCATCTTCATTCGAAGTTTCCCCCACACAATCGGAAAGTCCTTGGCAGATTATG TTTAACAACGCGTACAATTGGTGTGTCAAGTCGAATCAATAGATATTCTAGATGAATTGAAAGCAGTTAAATAGGTTCTT GGCATTTATGACCTGATATATCTAAATACTATATACACGAATTTGTTTACTATTTTTATAAAATAAACTTAAACACTCAA ACAACTTTAAAACTCAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.
Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.
RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.