CAAAAAATATTGACTTGTCTTACAAAACTGATTTTCATTGTTCGCTACTTAATATTTAGTGATAAATATATTACTTCGGG ACTGATAATCTTATGAATATACGTGTGAGATTTGGCGATACCCTTCATTACTTAGGAAGCCGAGCGCATAAGCTCACACC ATTATAATATTTAAGTGATATGAATATTAAATATTAAAATTTAAAACCAACTTTTTAATCAAAGAGTTTCCTTAAAATTG TTTAAGTTGCCGATCAAAAGTGGAAATTTATGCGTGCCGTTATTTGAAATACCAATCTTTTGAAAATGTTTACGCCGACG GTTAATATTATTTAAAAGGATAATTGAATTTATAAAACGACGTCATTCTTGTTGTGGACTAACTTTAATGAAATGGACGC CTACGCGTTACCTACATATTTTCCTCTTGCGTATTCTGAATTGCAGTTTTTAGCGTCCAGGAGAGCAGCTGCCGTCGCTG CAGCGGCTACTGTTTTACCAGGATCACCATGCATAAACCAACATCACCCAACTGACGTTTCAAGCTCGGTAACAGTGCCA TCAATTATTCCAACGGGTGGAACATCAGATTCAATTAAAACTTCAATACAACCACAAATATGCAATGAAAACACCCTTCT TGGAAATGCTGGCCACCAGCACAATCATCAGCCTCAACATGTTCACAATATAAATGTTACTGGACAGCCACATGACTTTC ACCCGGCCTATAGAATTCCCGGATACATGGAACAGTTGTATTCTCTTCAACGAACTAATTCAGCTTCATCTTTTCACGAT CCCTATGTCAATTGTGCATCAGCATTCCATCTTGCCGGACTTGGTTTGGGATCAGCTGATTTTTTGGGCAGCCGAGGTTT GAGCTCTTTGGGTGAACTGCATAATGCTGCTGTGGCAGCAGCAGCAGCCGGATCTCTCGCAAGTACTGATTTTCACTTCA GTGTTGATGGAAACAGGCGGTTGGGTAGCCCTCGACCACCAGGAGGAAGTATACGTGCCAGCATAAGTCGCAAACGAGCA CTTTCGTCATCGCCTTATTCCGATTCATTTGATATTAATTCAATGATTCGATTTTCGCCAAACTCTTTAGCCACAATTAT GAACGGTTCTCGAGGAAGCAGCGCGGCTAGTGGTTCTTACGGTCATATTTCTGCTACCGCCCTTAATCCCATGTCTCATG TTCATTCAACCCGTCTGCAACAGATACAAGCTCATTTATTGCGTGCAAGTGCAGGTCTGTTAAATCCGATGACACCACAA CAAGTTGCTGCATCTGGATTTTCTATAGGGCATATGCCAACCTCTGCTTCTTTGAGAGTGAATGACGTACATCCTAATCT AAGCGATTCACATATCCAGATAACAACATCCCCAACTGTTACAAAGGATGTAAGCCAGGTCCCAGCGGCAGCATTCTCGT TGAAGAACTTAGATGATGCAAGAGAGAAAAAGGGACCCTTTAAAGATGTTGTCCCCGAGCAGCCTTCATCGACATCTGGC GGCGTAGCACAAGTAGAAGCCGACAGTGCGTCTTCACAGTTGTCTGACCGTTGTTACAACAACGTGGTAAACAATATTAC AGGCATTCCTGGTGACGTTAAAGTAAACAGCCGCTTGGATGAGTATATTAATTGCGGATCAATTTCGATTCCGTCAAATG AATATGATTGTGCTAATGCTGATACTACGGATATCAAGGATGAACCCGGAGATTTCATTGAAACAAATTGTCATTGGCGT AGCTGTCGCATTGAGTTCATTACGCAAGATGAACTTGTTAAACATATAAATAATGATCATATTCAAACAAACAAGAAAGC TTTTGTCTGTCGATGGGAAGATTGTACACGCGGTGAAAAACCATTTAAAGCGCAGTATATGCTTGTTGTGCATATGCGGC GTCATACCGGTGAAAAGCCACACAAATGCACGTTTGAAGGCTGCTTTAAGGCATATTCACGTTTGGAAAACCTGAAAACT CATTTACGGTCACACACAGGAGAAAAACCATACACTTGCGAGTATCCGGGATGTAGCAAAGCCTTTAGTAATGCTAGTGA TCGCGCAAAGCATCAAAATCGAACACACAGTAATGAGAAACCGTACATTTGTAAAGCACCTGGATGCACAAAACGTTACA CCGACCCGAGCTCTTTGAGAAAACATGTAAAAACAGTCCATGGAGCTGAATTTTATGCAAATAAAAAACATAAGGGGTTG CCTCTAAATGACGCAAACTCTCGGTTACAACAAAACAACAGTCGGCATAATCTTCAAGAGCACAATATCGACTCAAGCCC TTGCAGTGAAGACTCACATTTGGGAAAAATGTTAGGCACGTCTAGTCCTAGCATTAAATCTGAATCAGATATAAGCTCAT CAAACCATCACTTAGTAAATGGAGTTCGAGCATCTGACAGTTTGTTAACATATTCACCTGATGATCTTGCTGAAAATCTT AATTTGGATGATGGCTGGAATTGTGACGATGACGTGGACGTGGCAGACCTACCAATTGTGTTACGTGCTATGGTAAATAT TGGTAACGGAAATGCATCCGCCTCGACTATTGGAGGTTCTGTTTTGGCTAGGCAACGGTTTAGAGGTCGTCTTCAAACTA AAGGTATAAATTCAAGTACGATTATGCTTTGCAACATTCCAGAAAGCAATCGTACCTTTGGAATTAGCGAACTTAATCAG CGTATTACAGAACTGAAAATGGAGCCAGGTACTGATGCTGAAATTAAAATTCCTAAGCTCCCAAACACGACCATAGGAGG ATATACGGAAGACCCTTTACAAAACCAAACAAGTTTTCGTAATACAGTATCGAATAAGCAAGGAACTGTTTCCGGATCGA TACAAGGACAGTTTCGTCGCGATAGTCAAAATTCCACTGCAAGCACTTATTACGGTAGCATGCAAAGTCGTCGTAGCAGT CAATCATCCCAGGTGTCTTCTATACCTACCATGCGACCGAACCCCTCGTGTAACTCGACTGCATCCTTTTATGATCCCAT TTCACCAGGGTGCTCACGGCGCTCTAGCCAAATGTCAAATGGTGCTAACTGCAATTCATTTACTTCCACATCAGGACTAC CCGTATTAAATAAGGAATCGAACAAAAGCTTAAATGCATGTATTAATAAACCTAATATCGGGGTTCAGGGTGTTGGAATT TACAATAGCAGTCTACCCCCACCTCCATCGTCCCACTTAATTGCAACAAACTTAAAGCGCTTACAGCGGAAAGATTCAGA ATATCACAATTTCACTAGCGGGCGATTTTCTGTTCCTAGTTATATGCATTCTTTACACATAAAAAACAACAAACCAGTCG GAGAAAACGAATTTGATAAAGCAATAGCGAGTAATGCACGACGTCAAACTGATCCAGTGCCAAACATAAACTTAGATCCA TTAACAAATATTTCTCGGTTCAGTACAACACCGCATAGCTTTGATATTAATGTCGGAAAGACTAATAATATAGCATCGTC AATTAATAAGGACAATTTAAGAAAGGATCTGTTCACTGTTTCAATTAAGGCTGACATGGCAATGACTAGTGACCAACATC CAAATGAAAGAATAAATTTGGACGAAGTTGAAGAACTTATACTTCCCGATGAAATGCTTCAGTACTTAAACTTGGTAAAA GACGACACAAATCATTTGGAAAAAGAACATCAAGCTGTACCTGTGGGGTCAAATGTATCCGAAACTATAGCTTCAAACCA CTACCGAGAACAATCAAATATATATTACACCAATAAACAAATTTTAACACCGCCTTCTAACGTTGATATACTACCAAATA CAACAAAGTTTACAGTACAAGACAAATTTGCTATGACGGCGGTCGGGGGTTCATTCAGTCAAAGAGAATTAAGTACCCTA GCTGTACCAAATGAACATGGGCATGCCAAATGCGAAAGCTTCCATCACCAGTCTCAAAAATATATGAACACTGACATTGG CTCCAAGCAGCAATCAGCATTACCGTCAGCTCATCAACGGCAAACAGAAAAATCAAATTATAATCAAATCATAGACTCAT CGATGACTAGCTTGCCAGAACTTAATGTAGATTCAATATATCCACGAAACGAAACAGAAAATATATTTAAAGTACACGGA GATCATGATAATGAAATCCAATGCGGCATTATAAGTCAATCTCAAATGTCACCTTCCACTAATTTGAATAATGATGGTCA GTTTTCTACCGTAAATATGCAACCAATAACAACATCCAAATTATTTCCCCCTGAACCACAAAAAATAGTTTGTGACACCC AAGCGAGTAATACTTCGGTTATGCATTTAGATACATATCAGCGTACTCTGGAATATGTACAAAGTTGTCAAAACTGGATG GAGACGAACAACACATCTACCAATCAAATACAAAGCCTCCCGGGAATGCCGGTAAACAATACATTGTTTCCCGATGTTAG CAGCTCTACACATCCATATCATGGGACAAATATGGTGATCAACGATATGACTACATCCCTTACTTCCTTACTCGAGGAAA ATAGATACCTTCAAATGATGCAGTAAAAAATGTTATCTAGCTAACACTGACAAGAAATCAGTCATAATAAACTATTTTAT TCTATTTAGATTGTATTTACTTTATAGGCAATATACCAATTACAAAACAAAATTAAATTAAAATCCTGATTCAACACTGT AATATTTTTTACAAATGTCCTTTAATCAAGTTCATTTATTATTTTACAAAAACGAGAATTTTATTTTAAATATTCTAAAT ACATGTAACAATATAATGTCTTCCGAGCTATTTTAAACATTAAAATGCAATGATTATGCTCTTACTAAATAAAAAAAAAA AAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.
Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.
RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
