CTGCCTCATCTTGAATTTGACTAAAAGTGCCGATTTCCAGCTCAACGAATAGTCCAAGTCGCGCCGAATTGTACAATTAA GTTAAAAAGTTCAACGTCAACTAGTTTATCATGGCAGATTCCAAGGGTGAAGATCTGGCTACGGCGATCCTGAAGCGCAA GGACCGTCCCAACCGACTGATTGTGGAAGAGGCGCAGAATGACGACAACTCTGTGGTGTCGCTATCGCAGGCGAAGATGG ATGAGCTGCAGCTCTTCCGTGGCGACACCGTGATTCTGAAGGGTAAGCGCCGAAAGGAGACGGTGTGCATCGTGCTCTCG GACGACACCTGTCCCGACGAGAAGATCCGCATGAACCGCGTGGTGCGCAACAACCTGTGCGTCCATCTTTCAGATGTGGT TTCCGTGCAGTCCTGCCCGGACGTCAAGTACGGCAAACGGGTGCGCATCCTGCCCATCGACGAAAGCACCGAAGGCGTTA CCGGCAACCTGTTCGAGATCTATCTGAAGCCGTACTTCCTAGAGGCCTATCGGCCAATCCACATGGGCGACAACTTTATT GTGCGCGCCGCCATGCGTCCTATTGAGTTCAAGGTGGTGCTGACCGACCCGGAGCCCTACTGCATCGTAGCTCCCGAGAC TGTAATCTTCTGCGACGGTGATCCAATCAAGCGCGAGGAGGAGGAGGAGTCCCTAAACGCCGTTGGCTACGATGATATCG GTGGTTGCCGCAAGCAGCTGGCCCAGATCAAGGAGATGGTTGAGTTGCCTTTGCGGCATCCATCGCTCTTCAAGGCCATT GGTGTGAAGCCACCGCGTGGTATTCTTATGTACGGTCCCCCGGGTACCGGTAAGACCCTGATTGCCAGGGCTGTGGCCAA CGAGACCGGAGCCTTTTTCTTCCTGATTAACGGACCGGAGATCATGTCCAAACTGGCCGGAGAGTCGGAATCGAATCTGC GTAAGGCCTTCGAGGAAGCCGAGAAAAACTCGCCGGCTATCATCTTCATCGATGAGATCGACGCTATTGCCCCCAAGCGT GACAAGACCCACGGTGAGGTGGAGCGTCGCATTGTGTCGCAGCTTTTGACCCTGATGGATGGCATGAAGAAGAGCTCCCA TCTGATCGTTATGGCTGCTACCAACAGGCCCAACTCCATTGACCCGGCCCTGCGTCGCTTTGGACGTTTTGATCGTGAGA TTGACATCGGCATTCCAGATGCCACTGGTCGGCTGGAGGTGCTGCGCATTCACACCAAGAACATGAAGCTGCATGATGAT GTTGACCTGGAGCAGATTGCTGCCGAATCCCACGGACATGTGGGCGCTGATCTGGCTTCACTCTGCTCTGAGGCTGCCCT TCAGCAAATCCGTGAGAAGATGGACCTCATCGATTTGGAAGACGATAAGATCGATGCCGAGGTGCTGGCGTCGCTGGCCG TGACAATGGAGAACTTCCGCTACGCTATGACCAAGTCCAGTCCATCGGCGCTGCGTGAAACTGTTGTTGAGGTGCCCAAC ACCACCTGGACAGACATCGGCGGTCTGGAGAGCGTCAAGAAGGAATTGCAGGAGCTGGTGCAATACCCAGTGGAGCATCC GGACAAGTTCTTGAAGTTTGGCATGCAGCCCAGTCGCGGTGTCCTTTTCTACGGACCCCCTGGTTGCGGTAAGACGCTGC TGGCCAAGGCCATTGCCAACGAGTGCCAGGCGAACTTCATCTCAGTCAAGGGTCCCGAGCTGCTGACCATGTGGTTCGGC GAGTCTGAGGCCAACGTGCGCGACATCTTCGATAAGGCTCGCTCGGCGGCTCCTTGTGTGCTCTTCTTTGACGAGCTGGA CTCGATCGCCAAAGCCCGTGGCGGTAATGTCGGCGATGCTGGCGGCGCCGCTGATCGTGTGATCAATCAGATCCTTACCG AAATGGATGGCATGGGAGCCAAGAAGAATGTGTTCATCATCGGAGCCACCAATCGCCCGGACATTATCGATCCGGCCATC CTCCGTCCGGGCCGTCTGGATCAGTTGATCTATATTCCGCTGCCCGACGACAAATCGCGTGAGGCTATCCTGAAGGCTAA CTTGCGCAAGTCGCCGCTGGCCAAGGAAGTAGACCTTACCTACATCGCCAAGGTGACGCAGGGCTTCTCTGGCGCCGATC TGACCGAGATCTGCCAGCGGGCTTGCAAGCTGGCCATCCGACAGGCCATCGAGGCTGAAATTCGTCGCGAGAAGGAGCGC GCCGAAAATCAGAACTCCGCGATGGACATGGACGAGGACGATCCAGTGCCGGAGATTACCAGCGCCCACTTCGAGGAGGC CATGAAGTTCGCTCGCCGTTCGGTGTCCGATAATGACATCAGGAAGTACGAGATGTTTGCCCAGACTTTGCAGCAGTCCC GTGGATTCGGACAGAACTTCAGATTCCCCGGTCAAACCGGCAACACCTCAGGATCTGGTAACAACTTGCCAGTTAACTCG CCCGGCGACAACGGCGACGATGATCTTTACAGTTAGATTTCTAGTTTTACACCGCAAAACAAAACCACAAAATGGAACTT TTTAAATGAATTTTTTCCGTAAAACCGAGCAAGCAAAACCAATCACATTCGCATCCTGGACGCGGTAACTTACTACAGCA ACCATTGGCTAAGACATCCAGCCGATGGCGAAATCAGATGACATACCCGATTCAATATTCACTTCTTTGATACCATCCTT ACTCTTGGTCGCGGATACGTGCAGTAGGAGAAGGTTCCCATTTGGATTTGGGCTCGCCTAATCCGAAACCATTTTAATTG GCGAACTATTCCGCGCCTCACACTAATCATTCGTGCATTGTCGGTAGAGTGGCTCCGATATATGAGTTTCTATAAACATA ATTATTAACGATACACATACACACATACATTTAAAGATAAATGCATATATTAAACACCGTAAAATTATAAGTGTATTTGG AGCTACATTTTTAAGTGAATAAAAATTATAAATTCAACAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.
Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.
RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
