FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\HL02012
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General Information
Symbol
Dmel\HL02012
Species
D. melanogaster
Name
HL02012
FlyBase ID
FBcl0150294
Feature type
cDNA_clone
Computed gene(s)
Dmel\ap
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pBS_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,368
      NCBI
      BT005201
      Full Length sequence dataSequence Downloader
      CGGGCATCTAATCGTGGCCAGTGTCAAGTGTCCAATAGCACATCTAGCCGGGGTGTAGAGGATTACCCAAGACCAACGTA
GCACCGCAGCCCTTTTAAGGAGGCATAAGGCTCGCAAGACACAACGCACAGCCTACACAACGACAAACATAACACGACCG
ACGGACATCATCCTTGGAAATAGACCTCGTGTCATTCCAAAAGACGCACATGGGCGTCTGCACCGAGGAGCGCCCTGTGA
TGCATTGGCAGCAGAGCGCAAGATTTCTTGGGCCCGGCGCAAGGGAGAAAAGTCCAACACCACCTGTAGCACATCAAGGG
AGCAATCAATGTGGCAGTGCTGCAGGTGCAAATAACAATCACCCATTGTTCCGCGCGTGCTCCTCATCCTCGTGTCCAGA
TATTTGTGATCATAGTACAAAGCCATTTGGCAACGCTTACGGCACCGAGTCATTTAGAAGCTACGAAACAGCCGATCGTG
CTACCTTTGAGGACTCAGCCGCCAAATTCTCCATCAGCCGCAGTCGAACAGACTGCACGGAGGTCAGCGACGAGACGACG
TCGGGCATATCATTCAAGACCGAACCCTTCGGACCGCCCAGCAGTCCCGAGTCCACAAGCGATAGCAAAATAACGCGCAA
CCTCGACGACTGCTCCGGCTGCGGACGTCAGATACAGGATCGCTTCTACCTCTCCGCTGTGGAAAAACGGTGGCATGCAA
GTTGCCTACAGTGCTACGCCTGTCGGCAGCCGCTGGAACGGGAATCCTCATGCTACTCACGTGACGGCAACATTTATTGC
AAAAACGATTATTATAGTTTTTTTGGTACTCGCCGATGCTCGCGCTGCCTGGCCTCCATCAGCTCCAACGAGCTGGTCAT
GCGCGCCAGAAATCTTGTTTTTCACGTCAACTGCTTCTGCTGCACTGTCTGCCACACGCCACTGACAAAGGGAGACCAGT
ACGGCATCATCGACGCCCTCATCTACTGCAGGACCCACTACAGCATAGCCAGGGAGGGGGATACCGCCTCATCCAGTATG
AGCGCCACCTACCCGTACAGCGCCCAGTTCGGCTCACCCCACAACGACTCCTCGAGCCCGCACTCGGACCCTAGTCGGAG
CATTGTTCCTACGGGCATCTTTGTGCCCGCGTCCCACGTAATCAACGGACTGCCGCAGCCAGCTCGTCAAAAGGGCAGGC
CCCGCAAGCGCAAGCCCAAGGACATCGAGGCGTTCACCGCTAACATAGATCTCAACACTGAGTACGTGGACTTTGGCCGA
GGCTCGCACCTAAGCTCCTCGTCGCGCACCAAACGAATGCGAACCTCGTTTAAGCATCACCAGCTGCGTACCATGAAGTC
CTACTTTGCCATTAATCATAACCCTGATGCAAAGGATCTAAAACAATTGTCACAGAAAACTGGTTTACCAAAGAGGGTCC
TACAGGTCTGGTTTCAAAATGCAAGGGCCAAATGGCGCCGCATGATGATGAAGCAGGATGGCAGCGGCCTGCTGGAGAAG
GGCGAAGGCGCTCTGGACCTCGACAGCATCTCCGTGCACAGTCCTACGTCGTTTATATTGGGAGGACCGAACAGCACACC
GCCACTTAACTTGGACTAACGGATGCTCAGGAAACGCGAAAACAAAATGATAGTGACAAAGGAAATGGGCGCAGCAGTCA
CCTCCGACATCATACGTTCTTATGCCAGTTGTCTATTCGCGTGCCTCATACACAGTCCCGGACCCGGTTTGACTACTGAA
TCCGGATACTTGTCTAGATCAAGCACGGCTAGCTCTTGAATGCTATGGGCTGCCTTGTCGGATGGGAATCAATTTGGAAA
GCTTGTCTTGTGTTCAAAATCGAAAAATTGATTCATCCACACGTTAAACGCCCGGGAAACGCCTCGGAAAATGCTAAGAA
AATATGTATTTTTGTATTTGCCTATTGACCGTTTCCAGCACTACTCGCCTCGTCCTTGCCCCACATGTTGTTTCTATAGT
TTTAACGTTCACTTGTAAATATGTACTGTAATCACATAGTTTATTTCAAACGATCCATATTAGCCTGACTCCCCTGTTTT
TTGTATTCTTTTAAAGCGCAGAATATCAATTCGAACATTGAAATTATGAATCAAAAGAAGGTGGATTCATGCAAAGATTA
CCAACTTGTAACGTTCTTTCTAAAGCTGTCATAATAATTAAGAAAACACAACTTCGCTAAATTTCAATTTGTATAGAAAA
TAGTTATGTTACTATACCAGTCAAAATTTTTAAACATCAAACTTGCAGAATTTACGAATTAAAGTATGACTAAAAACTTT
TAAATACCAAACAGCGAAACGTGTGATATTTTAAATTTGTTTTTTTCTTTTTCAGTGACACGACTAAAAGATACCCGTTA
AATTGTAAAATAATCTTGCCAATATATATGCGGCCAGGTCATGCCCTTCCTCATATGAATTGCCCGCTGGCAATGTTTTT
ACCAAGCTTTCACCTTCCGATTAGGTTTGGTGGTCATTTAAGTAAATTCCGATTTAAACTTTGTTTTTAATACGGGAGCG
TGTCACTTCCTTTCGGTATGAACATAGGTGAGGAAAAGTTTGAGGCCAATCTTACGGCGCACAAGCGGCACACAAATGAA
ATGAAATTCTTTAGCTTTTATAGTAGACGAAAAACGATCCTTATCAAGCATAAATGCTTATATCTCCTGTAACATTATTT
TTGACCAGTCTAGTTTACACGTATGTTCTACGAGGAACGGACCGATTGTTCCTATGAGAGCTATAGGTTTTACCATATGT
ATATGCATGCATTACTTCAGCTCCATCTCTTTTTGATTCATAAACGTATAATGGTTTGAGAGTAAACATTTTGTGGCGTA
TATAGTTCAAGTTTACATATTTAGAGTACGGTGCCCAAGACGCGCTTTAAAAATATTGAAAACGGGTTGTTAATGAAGTC
CCATATGTATAATTGTCTAGGTACATGGATATTGTTAATATATTATTATTTTTATTTAAAGAGCTCTCAACAATATGTAT
GAAGAAATCTGCGTATATCAACATATAATGAAATGCTGATGCATTCGTATTATTATATGTAACTAATATTTATTGCCCAA
ATTTAATAGTTAGAGACGAATTTAGTTTAGAACAGACTATACAATAAAGTCCATATGTACATGTAGTATTACGGTAAAGT
AATAGCTTCGCTCAAGCATACGACCAGGTTCGTAAGCTATTAATACGAAACTGTAATTATAATCAAATCAGTAAACACTG
GAAGATTTGTACTTACAAATAGTTAACTCATAAGTCTTACCATAGAAGCTTAAATAAAACGTATTCAAGCAAAAAAAAAA
AAAAAAAA
      Library Information (1)
      Library: HL_pBS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pBS
      (Rubin et al., 2000)
      HL_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)