Comment: 0-22 hr AEL
CCGCATCAAGTCCTCGTCCTCCGGCGTGGCCAGTGGCAGCGGATCGGGCGGAGGCGGTGGCGGATCCGGATCGGGGCTGA GCCAGCGTAGTGGCGGACACAAGGATGCACGCTGCAATCCCACCGTGGGTCTCAACATATTCACCGAGCATAACGAAGCC CTGCTGCAGTCGCGTCCATTACCTCACATTCCGGCCGGCAGCACGGCGGCCTCTCTTCTGGCGGATGCCGCTGAGCTGCA GCAGCATCAGCAGGATTCCGGTGGACTGGGACTGCAGGGCTCCTCCCTGGGCGGTGGTCACAGTTCGACTACATCCGTGT TTGAATCCGCCCATCGGTGGACCTCGAAGGAGAACCTACTGGCCCCCGGACCCGAGGAGGATGATCCGCAACTTTTTGTG GCGCTATACGATTTCCAAGCCGGCGGAGAGAATCAATTGAGTCTGAAGAAAGGCGAGCAGGTGCGCATACTTAGCTACAA CAAATCGGGGGAGTGGTGCGAGGCGCACTCGGACTCCGGAAACGTTGGATGGGTGCCCTCCAACTATGTCACGCCGCTCA ATTCGCTGGAGAAGCACTCCTGGTACCACGGGCCTATCTCACGCAACGCCGCCGAGTATCTTCTCAGCTCCGGAATCAAT GGCAGTTTCCTGGTCCGTGAAAGTGAAAGTTCACCGGGTCAAAGGAGCATCAGTTTGAGATACGAGGGTCGCGTCTATCA CTACCGCATCTCAGAGGATCCCGATGGGAAAGTCTTCGTTACCCAGGAGGCCAAGTTTAACACTCTGGCGAGCTGGTGCA TCATCACAGCGTGCCCCATGAGGGTCACGGCCTGATCACACCGCTCCTATATCCGGCGCCCAAGCAGAACAAGCCCACCG TCTTTCCGCTGAGTCCCGAGCCGGATGAGTGGGAGATCTGCCGGACGGACATCATGATGAAGCACAAGCTAGGCGGCGGT CAGTACGGAGAGGTCTACGAGGCCGTTTGGAAGCGGTATGGCAATACGGTGGCTGTTAAAACGCTCAAGGAGGACACCAT GGCACTGAAGGACTTCCTCGAAGAGGCGGCCATCATGAAGGAAATGAAGCACCCTAATCTGGTGCAGCTCATTGGTGTTT GCACCAGAGAACCACCGTTCTATATCATCACCGAGTTTATGTCGCACGGCAATCTGTTGGACTTCCTGCGCTCCGCCGGA CGCGAAACGCTCGATGCAGTAGCGTTGCTGTACATGGCCACTCAGATAGCGTCGGGAATGAGCTACCTGGAGTCGCGCAA CTACATTCATCGCGATCTCGCTGCCCGCAATTGCCTGGTGGGTGACAACAAGCTGGTCAAGGTGGCGGATTTCGGCCTGG CACGTTTGATGCGGGACGACACGTATACAGCACATGCCGGAGCCAAGTTCCCGATCAAATGGACCGCACCGGAGGGTCTG GCCTACAACAAGTTTAGCACCAAATCAGACGTTTGGGCCTTCGGAGTCTTGCTGTGGGAAATCGCCACGTATGGAATGTC ACCGTATCCGGCCATCGATCTGACCGATGTGTACCACAAGCTAGACAAGGGCTATCGCATGGAGCGACCGCCAGGCTGCC CGCCGGAGGTGTACGACTTGATGCGCCAGTGCTGGCAGTGGGATGCCACCGACAGGCCCACGTTCAAGAGCATACACCAT GCGCTGGAGCACATGTTTCAGGAATCGTCCATCACCGAAGCGGTGGAAAAGCAGCTGAACGCCAACGCCACCAGCGCGAG CAGCTCCGCTCCGAGCACATCGGGCGTGGCCACCGGCGGAGGAGCCACAACCACGACGGCGGCCAGCGGCTGCGCTTCCT CATCCTCGGCCACCGCCTCGCTAAGTCTCACACCGCAGATGGTGAAGAAGGGTTTACCCGGCGGTCAGGCCCTCACGCCG AACGCCCACCACAACGATCCGCACCAGCAGCAGGCCAGCACGCCCATGTCAGAAACCGGATCCACTTCCACCAAGCTAAG CACTTTCTCCAGCCAGGGCAAGGGCAATGTCCAGATGCGTCGCACCACCAACAAGCAGGGCAAACAGGCGCCCGCCCCAC CAAAGCGAACCAGCCTGCTCTCGAGCAGTCGGGACTCCACTTATCGCGAGGAGGATCCAGCCAACGCCAGATGCAATTTC ATCGACGACCTCAGCACGAATGGACTAGCCCGGGACATCAACAGTTTGACGCAGCGGTACGATTCCGAAACAGATCCGGC AGCCGACCCGGACACAGATGCCACGGGCGATAGTCTGGAGCAGAGTCTGAGCCAAGTGATAGCCGCTCCGGTCACCAACA AGATGCAGCATTCTCTTCACAGCGGAGGAGGAGGAGGAGGAATAGGTCCTCGATCCTCGCAGCAACACAGCTCCTTCAAG CGACCGACTGGAACACCCGTGATGGGTAACCGAGGACTAGAGACCCGGCAGAGCAAGCGGTCCCAGCTCCATTCACAGGC TCCGGGTCCAGGACCGCCATCAACTCAACCGCATCATGGCAACAACGGCGTGGTGACCAGTGCTCATCCCATCACTGTGG GTGCGCTCGATGTGATGAATGTCAAGCAGGTGGTGAACCGCTACGGAACACTACCAAAGGGTGCCAGAATCGGTGCCTAT CTGGACAGTCTTGAGGATAGCAGTGAGGCTGCTCCTGCTCTTCCGGCAACTGCTCCTTCTCTGCCACCAGCCAATGGACA CGCCACGCCTCCGGCTGCCAGACTAAATCCGAAGGCCAGCCCCATTCCGCCACAGCAAATGATCAGGAGCAACTCGTCGG GCGGAGTGACCATGCAAAACAATGCGGCTGCCAGCTTAAACAAGCTTCAGCGTCATCGCACCACTACCGAAGGCACCATG ATGACGTTCTCCTCCTTCCGGGCGGGCGGTTCCAGTAGCTCACCCAAGCGGAGTGCCTCGGGAGTGGCTTCAGGAGTCCA GCCAGCTCTGGCCAACCTTGAGTTTCCACCGCCTCCGTTGGACTTGCCTCCGCCGCCCGAGGAATTCGAAGGCGGACCAC CACCTCCTCCACCGGCGCCGGAGAGCGCTGTGCAGGCCATCCAGCAGCACTTGCATGCCCAGCTGCCAAACAATGGCAAC ATTAGCAATGGAAACGGAACAAACAACAACGACAGCAGCCACAACGATGTTAGCAACATAGCTCCCAGTGTGGAGGAGGC CAGCTCCAGATTCGGTGTGTCTCTGAGAAAACGCGAGCCCTCCACCGACTCCTGCAGCTCGCTGGGCAGTCCACCCGAGG ATCTCAAGGAAAAGCTAATCACCGAAATCAAGGCGGCCGGCAAGGATACTGCTCCGGCCTCACATCTGGCCAACGGCTCG GGCATCGCTGTCGTGGACCCCGTCTCCCTGCTTGTCACCGAACTAGCCGAGAGCATGAACCTGCCAAAGCCGCCGCCGCA GCAGCAGCAAAAGCTGACCAACGGCAATAGTACTGGGTCCGGATTCAAGGCTCAGCTGAAGAAAGTCGAGCCCAAAAAGA TGAGCGCGCCAATGCCCAAGGCGGAGCCGGCAAATACCATCATCGACTTCAAGGCTCATCTGCGCCGGGTGGACAAGGAA AAGGAGCCAGCAACTCCAGCTCCAGCTCCAGCTACTGTAGCCGTAGCCAACAATGCCAACTGCAATACAACGGGCACTTT GAACCGGAAGGAGGACGGCAGCAAGAAGTTCTCGCAGGCCATGCAAAAGACTGAAATCAAAATCGACGTAACAAACTCGA ATGTGGAGGCGGATGCGGGAGCAGCGGGCGAGGGCGATCTCGGCAAGCGGCGAAGCACAGGTAGTATTAATAGCTTAAAG AAACTGTGGGAGCAGCAGCCACCGGCGCCGGATTATGCCACCAGCACGATCCTCCAGCAACAGCCGTCGGTGGTAAACGG AGGCGGAACACCAAATGCCCAACTGTCTCCCAAGTATGGCATGAAATCGGGGGCCATCAATACGGTTGGCACTCTTCCAG CCAAACTGGGCAACAAGCAGCCACCGGCTGCCCCTCCACCACCGCCCCCGAACTGCACCACCTCCAACTCCTCCACCACA TCCATTAGCACCTCTAGTAGAGATTGCACCAGCAGGCAGCAGGCCAGCAGCACAATAAAAACCTCTCATTCAACGCAACT CTTCACAGATGACGAGGAGCAGTCGCACACGGAGGGCCTGGGATCGGGAGGCCAAGGATCAGCAGACATGACCCAGTCGC TGTACGAGCAGAAGCCACAGATCCAGCAAAAGCCAGCGGTGCCGCACAAGCCAACAAAGCTAACCATCTACGCCACGCCT ATTGCCAAACTGACCGAACCAGCCAGCTCCGCCAGCTCCACACAGATATCACGGGAGAGCATTCTGGAGCTGGTTGGCCT GCTAGAGGGCTCGCTCAAGCACCCGGTGAATGCCATCGCTGGATCTCAGTGGCTGCAGCTGAGTGACAAGCTCAACATCC TGCACAATTCGTGCGTGATCTTCGCGGAGAACGGAGCGATGCCGCCGCACTCCAAGTTCCAGTTCCGGGAGCTGGTCACG CGCGTGGAAGCACAGTCGCAGCACTTGCGCTCCGCCGGCAGCAAGAACGTCCAGGACAACGAGCGCCTGGTGGCCGAAGT CGGTCAGTCGCTGCGTCAGATCTCCAATGCGCTTAACAGGTAAATGATAAGACGGGGGATGGACGCGCCCGACCAACATC AGGGCATCTGGCTGGATGCCGAGGGTAACTTCATTAAGCGCAAGTGATCGGGCCCCAATTTGTAGACCCAATCGTCCAAC CACACCATCCCCCGTGGAAGCGGAACTGGAAACGGAAATGGAAGCAAAGATAGCAGGCCGGAAACAGGAGGAGCAGAAAC AGGAAACGAAAATTCAAGCCCAGCGAAGAAGAAATGACAACTACATTAGCAATAGCAGCAACTAAAATCGGAGGAGGACA CCTATGTATCGTACTTGGTAGCGTGTAGTAGTAGCTTCTATTATATATACCCATATATATCTAGTCAGATCTGTGCAGAG CCCCGACGAACGCTTCCAGAAAACCAAGAAATTAGAAAACCGAAAACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
