Comment: 0-22 hr AEL
GCGCCTAGTGACTTCGATACTTCAACTTCGATATGTAAACACAATTGCCATCTCTACCAGCACCGAGCCGAAATAAAATA AGTGCAACGCGCAGCGTCTAATTGAAACCAAATTGATCTATTTGGCCGCGGCCGAAACTGGACATTCGCAATTTGGGCGG TCAGCCGCCCCCGTCCCGTCCCGCCCCCCCGTGCGTTTCCCCCGCTGCCGACGGACAGTACGTCGGTGTGACGCACATCG GTGTGTGCGCGCGTGTGTGTGTGTCTGTGAAGCGCCTTGTTATTGTTGTTGTAGCGCTTGCTACGTTCGCCGTTCGCAGT GCACTGGTGTGCGTGCGCTCGTGTAAGTGTGTGTGCACAGAGGTATCGTTGGAATTAACAAAAAAATAGAAGAAGGAAAA AGCTACTTGATTGGGGCACACAAAAACCAACAACTCAAAAGGCCGAGAAACAAAAGCCCACCTTCAACACCACCAATCCG CCGCATTCGACACATTTTGGCCAGGTTTTTGGCCACAAGTGTTGACACAACTGTTGCATGCCAAGCGGACTGCGGACAAT AATCCCCGTTCCCTTGTTTACCCAACAATCCATCGTGGTCAACGTGATCATCCTGGTCATTACGATCCCATTTGCACAGG AAGTAAACTAGATTGATAGCATTTTCCGGGAATCGATAGCACACCTGCCTGCCGTGTGGGAAACGCACCTGGGAACGCCC GGGTGCTCGGCTGCGTCCGCATGTGGCGTTGGCGGCACTCCACCAAAAGAAGCCAGCGCCAAGTGCCCAATCCCCATACC CATACCCATTCCCATTCCCATCCCCATCCGTATTCGATTGGCGTTCAGTGGCAGCAGTTAGCCAGAAATCCAACGAAACA CTCGTGACCTCATCGCCAGCAAACAGCAAACAACTAGCGCTTGACGCGTGCCTCCTCTCCTCATTACTATTAGCATTCCC AGCATAGACAGACGTCCAACAACTCCTGGCAGTTGCAGCACAGGAGCAGCAGTTGACCAACAGGAAGCAGCAGCTGGAGC AGCAGTATCAGCTTGTTATCTTGCATTTGCATGCCAGCTGGACGAGAAACTGTGACCAAGCAGCGCAGCATGGATGTGGA ACGAAGGCGCCGGGCGAATACGCTCGAGCGCGAGATGCGCGATCCGACCAGCATCTGCAATGTGGACTGCCTGCTGGACA CGGTGTCGGCCTTGGTCAGCGATTGTGACCACGAGTCCCTGAGGCGGCTTAAGAACATCGAGCAGTATGCGGCCAAATAC AAACCATTGGCAATGCAGATCAATCAGCTGCGCATGAACGTCGAGGATTTCCATTTTATCAAACTCATCGGCGCCGGCGC CTTTGGAGAGGTGCAGCTGGTGCGGCACAAGTCCTCCAGCCAGGTGTACGCCATGAAGCGGCTGTCCAAATTCGAGATGA TGAAGCGACCGGACTCCGCGTTCTTCTGGGAGGAGCGACACATTATGGCGCACGCCAACTCCGAATGGATCGTTCAGCTG CACTTTGCATTTCAGGATGCCAAATACCTATACATGGTGATGGACTTTATGCCCGGCGGCGATATAGTCTCGCTGATGGG TGACTACGACATCCCGGAGAAGTGGGCCATCTTTTACACAATGGAGGTGGTTCTCGCGCTGGACACCATTCACAATATGG GCTTCGTGCATCGGGACGTTAAGCCGGATAATATGCTCCTCGACAGCTATGGCCACCTGAAATTGGCCGACTTTGGCACC TGTATGCGAATGGGCGCCAATGGCCAAGTGGTGTCCAGCAATGCCGTTGGCACGCCGGACTACATCAGCCCGGAGGTGCT GCAGTCGCAGGGCGTGGACAACGAGTACGGGCGGGAATGCGACTGGTGGTCGGTGGGCATTTTCCTCTACGAGATGCTGT TCGGCGAGACACCATTCTACGCAGACTCGCTGGTGGGCACCTACGGCAAGATCATGGACCACAAGAACTCGCTTAGCTTT CCACCCGAGGTTGAGATCAGCGAACAGGCGAAGGCACTGATCCGCGCCTTTCTCACGGACCGAACGCAACGTTTGGGTCG CTACGGCATCGAGGACATTAAGGCGCATCCGTTTTTCCGAAATGATACCTGGTCGTTCGACAACATTCGCGAGAGTGTGC CGCCTGTGGTGCCCGAACTCTCCTCCGACGATGATACGCGAAATTTCGAGGACATTGAGCGCGATGAGAAGCCGGAGGAA GTGTTTCCCGTACCCAAGGGCTTCGATGGCAATCACTTGCCCTTCATCGGCTTTACGTATACGGGTGACTATCAGCTGCT GTCTAGCGACACCGTGGATGCGGAGTCGAAGGAGGCCAATGTGGCAAACAGCGGGGCGGCCAGCAATAATCACGGCCACG GTCATAATCACCGCCATCGGCCGTCGAATTCCAACGAGCTGAAGCGCCTGGAGGCGCTGCTCGAGCGGGAGCGTGGCAGG TCGGAAGCCTTGGAGCAGCAGGATGCTGGACTGCGGCAGCAGATTGAGCTGATAACAAAACGCGAAGCGGAGCTGCAGAG GATTGCCTCAGAGTACGAGAAGGATCTGGCTTTGCGTCAACACAACTACAAGGTGGCCATGCAGAAGGTGGAGCAGGAGA TCGAGCTGCGCAAGAAGACGGAGGCACTGCTCGTGGAAACGCAGCGCAATCTGGAAAACGAGCAAAAGACGCGGGCACGC GACCTCAATATCAACGACAAGGTGGTCTCACTCGAAAAGCAGCTGCTTGAGATGGAGCAGAGCTACAAAACGGAGACGGA GAACACTCAAAAGCTGAAAAAGCACAATGCCGAGCTGGACTTCACGGTCAAGTCGCAGGAGGAGAAGGTGCGCGACATGG TCGACATGATCGACACGCTACAAAAGCATAAGGAGGAGCTCGGCCAGGAGAATGCCGAGCTGCAGGCGCTGGTGGTGCAG GAGAAGAATCTGCGACCGCAGCTCAAGGAGATGCACAAGGAGGCCGAGAATAAGATGCAGACGCTGATCAACGACATTGA ACGTACTATGTGTCGCGAACAAAAGGCGCAAGAGGACAACCGGGCGTTGCTTGAGAAGATCAGCGACTTGGAGAAGGCGC ATGCCGGCCTCGACTTCGAACTGAAGGCGGCCCAGGGCCGCTATCAGCAGGAGGTCAAGGCACACCAAGAGACGGAGAAG TCGCGGCTCGTCTCCCGCGAGGAGGCCAATCTGCAGGAGGTAAAGGCGCTGCAGTCCAAGCTCAACGAGGAGAAGTCCGC TCGCATCAAGGCCGATCAGCATTCTCAGGAAAAAGAGCGTCAGCTGAGCATGCTGTCCGTGGACTATCGCCAAATCCAAC TACGTCTCCAGAAGCTCGAAGGCGAGTGCCGCCAGGAGTCGGAGAAGGTGGCCGCTCTGCAGTCACAGCTCGACCAGGAG CACAGCAAACGCAATGCCTTGCTATCGGAACTCAGCCTGCACAGCTCGGAGGTGGCCCACCTGCGATCCCGCGAAAACCA GCTACAGAAGGAACTGTCTACCCAGCGCGAGGCGAAGCGACGCTTCGAGGAGGACCTCACCCAGCTAAAGAGCACACACC ACGAAGCGCTGGCCAATAACCGTGAGCTTCAGGCCCAACTCGAGGCGGAGCAGTGCTTCTCGCGGCTGTACAAGACACAG GCGAACGAGAATCGCGAAGAGAGCGCTGAGCGGCTGTCGAAGATCGAGGATCTTGAGGAGGAACGCGTCTCACTCAAGCA CCAGGTACAGGTGGCCGTTGCCCGTGCAGACTCAGAGGCTTTGGCCCGATCCATTGCCGAAGAGACGGTGGCGGATCTCG AAAAGGAGAAGACTATCAAGGAGCTAGAGCTGAAGGACTTCGTCATGAAGCATCGCAACGAGATCAACGCCAAGGAGGCG GCCCTAGCCACGCTCAAGGAGGCCGAAAACGAACTGCACAAGAAGCTGGGCCAGAAGGCAGCCGAGTACGAGGATCTTGT CCAGCAGCACAAGAAGCAGCAGGAGGAGCTTGCGCTGATGAGAAGCAGCAAGGACGAAGAGATCACCAAGCTGCTGGATA AGTGCAAGAACGAAGTGCTTCTCAAACAGGTGGCAGTGAACAAGTTGGCCGAAGTGATGAATCGTCGCGACTCCGACCTG CCCAAACAAAAGAACAAGGCGCGCTCGACAGCCGAGCTGCGCAAAAAGGAGAAGGAGATGCGTCGGCTGCAGCAGGAGCT CTCGCAGGAGCGCGACAAGTTCAACCAGCTGCTGCTCAAGCACCAGGACCTGCAGCAGCTGTGCGCCGAGGAGCAGCAGC TCAAGCAGAAGATGGTCATGGAGATCGATTGCAAGGCCACCGAGATCGAGAACCTGCAGAGCAAGCTCAACGAGACGGCG TCGCTCTCCTCGGCGGACAACGATCCCGAGGATAGCCAGCACTCCTCTCTGCTCTCCCTCACACAGGACTCGGTCTTCGA GGGCTGGCTAAGTGTGCCCAATAAGCAGAATCGGCGACGTGGCCACGGCTGGAAGCGGCAGTACGTCATTGTGTCGTCGC GCAAAATCATCTTCTACAATTCGGACATTGACAAGCACAATACCACGGATGCCGTGCTCATCCTGGACCTGAGCAAGGTG TACCACGTTCGTAGCGTCACTCAGGGCGATGTCATACGCGCCGATGCCAAAGAGATTCCACGCATATTCCAGCTACTGTA CGCCGGCGAGGGCGCCTCCCATCGTCCCGACGAGCAGAGCCAGTTGGATGTGAGCGTGCTGCACGGTAATTGCAACGAGG AGCGCCCGGGCACCATTGTCCACAAAGGTCACGAGTTCGTTCACATTACCTACCACATGCCCACGGCTTGCGAGGTGTGC CCGAAGCCTTTGTGGCACATGTTCAAGCCGCCGGCGGCTTACGAATGCAAGAGATGCCGCAACAAGATACACAAGGAGCA TGTGGACAAACACGACCCGCTGGCGCCCTGTAAGCTCAATCACGATCCGCGCAGCGCCCGTGACATGCTGCTGCTGGCCG CCACGCCCGAGGATCAATCGCTGTGGGTGGCGCGCCTGCTGAAGCGTATCCAAAAGAGTGGATATAAGGCGGCTTCGTAC AACAACAACAGCACCGATGGCAGCAAGATATCGCCCAGCCAATCGACGCGCTCCTCCTACAAGCCGTATGCGGTTAATGT CCAACGCTCCGCCACGCTGCCAGCCAATTCATCGCTGAAATGAACGCCAACCGATCCGATGCGATCCCAGATCCCACATC CCAGATGCCACATGCCAGATCGCAGGCGTGGCAATGCAGCTTGAGCCGGAGCAGTCGCGACTCTCCAGCCCCAACGAAAC TAGCGCTATGCAAACTGAAACTGATACAGAAAGAAAATAACTGAGAGAAAAAGAGAGAGCAACTGCAACTGCAACTTGAA TGGCAACTGCAACTACTATCTTAAATATACGCTCCAAACATACATGCTACAGTAATCTACAAATATTTTTTGAGTAATTT ACAAGCTAAACTAAACTACAACTAAACTAAAACTAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
