Comment: 0-22 hr AEL
TTTTTTCGGATCGGCAAAGCCGCGTGCGCCCCATCGCACTCTCGCACACACACATACGCACTCACAGAAAAAGATATTAT GCATACATATGTATGTTTAATATATATATTTCTCTGATTGGATTGGGGCAAAAACAAAACCCAAAGAGTGCGAAAAAGAG AGAACGCGAGTAAATAGCAACCGGAATAACAAAAAGCACAAAGTGAACAATCGTTAAACAACCAAAAAGCAAGCCAATTA GAAAAATAAACACTGCACAAAAATCCAACAAAAAACAGGAGAATAGAAAGGAAAACGCCGTGATCGTGAAAAACAAAAAA CTTTATCTAACTGTAAATCCAAACGCCAACGTGGGAGGCATCCGAAGCGAAAACATCGGATGAGCAGGCAGAAAAGAGAG GGAAGAACGATCTCTGGCAAAAGCTGATGACAAAACGAGCAGTCAACCTCTCCGGTTCAATATAAACATTGTTAATATAC CTTCAACGAAAAGCGTTGAAATCGATTCCAAGGACTATATGTTTCAGGGTGAAACACGACCCACATATTTCCTTCACCAA AATTGTCCTTCACAACAAAAGAAGTTATATTTATTTGACGGAAATCAAAATTAATATACCTGGATCTGTGGCTGCGACTA CACCAACAATTGCAGCACATACTTACACACTATACAACAAACATATCGCTGTGAGAATCCGTTGGGACCCATAGGCGTCG CGCTGAACGGCCCCGGCCTAAGGGGCCATGACGCGTTGGCCCTTTAATCTACTACTCTTGCTGTCGGTGGCGGTACGCGA CTGTAGCAATCATCGCACCGTCCTCACTGTCGGCTACCTAACGGCCCTCACTGGCGATCTCAAGACGCGTCAGGGACTGG CCATTTCCGGTGCCCTGACTATGGCCCTGGATGAGGTCAACAAAGATCCCAATCTTCTGCCAAATGTGTACCTGGATCTG CGCTGGAATGACACCAAGGGGGACACGGTACTGGCCACCAAGGCCATCACCGAGATGATATGCGATGGCATCGCCACCAT TTTTGGCCCGGAAGGACCTTGCTATGTGGAGGCCATCGTCTCGCAGAGTCGAAACATTCCAATGATTTCCTACAAATGTG CAGAGTACCGTGCATCCGCCATACCGACATTCGCCCGCACCGAGCCGCCAGATACACAGGTCGTTAAGTCGCTGCTTGCC CTTCTGCGATATTATGCGTGGAATAAGTTTTCCATTCTGTACGAGGATGTGTGGAGTCCGGTGGCTGATCTGCTGAAAGA TCAGGCCACCAAGAGAAATATGACGATCAACCACAAGCAGTCGTTCATTGATAATCGAGTCAAGTGCTGTGAGCAGATGC TGGACTGCTGTCGTTCTGGGTATTGGTACCAGCTGGTGCAAAACACGATGAATCGCACACGAATCTACGTATTTCTGGGG GCGGCGAATAGTCTGGTGGACTTTATGAGTTCCATGGAGACCGCTGGTCTGTTTGCCCGTGGCGAGTATATGGTGATCTT TGTCGACATGATGGTCTATTCCGAGAGGGAGGCAGAAAAGTACTTGCGCAGAGTGGATCAAATAACATTCATGTCCAATT GCCATAGCACGGAGAACTTCAATCAAATGGCCCGCAGTCTTCTCGTCGTGGCATCCACGCCACCCACAAAGGATTACATA CAGTTCACTAAACAAGTGCAGAAGTACAGCTCAAAGCCGCCGTTCAATTTGGAAATTCCCAGGCTGTTTGTAGAAAGTAA TTTTAGCAAGTTCATATCGATCTATGCCGCGTACCTGTACGATTCGGTAAAGCTGTATGCCTGGGCTGTGGACAAAATGC TGCGGGAGGAGACCCGAGTCTTGACCGATGATGTGATCTTCGAGGTGGCCAGCAACGGAACACGTGTAATCGATACCATA ATCAAAAACCGTACTTATATGAGCATAACTGGATCCAAAATTAAGATCGATCAATATGGCGATTCGGAGGGTAATTTTTC GGTCCTGGCCTACAAGCCACACAAGTGGAATAACTCGAACAACATGCCCTGCAACTATCACATGGTTCCTGTAGCTTACT TTCATCAAGGCGAAGAACATCCAGAGTACAAGCTCATCAATGGCTCCATAGATTGGCCATCGGGCGGTGAGAAGCCCGCA GATGAACCGATGTGCGGTTTTGCAAACGAGCTGTGTAAAAAGGACGACACCCACTATACGTCCACTGTCGCCGCTGTGGT TTTGGGCGTATTGCTCTTCTGCTCAGGTGTTATCACCATGAGTATATATCGGAAGTGGAAGATCGAGTTGGAGATTGAGG GCTTACTCTGGAAGATCGATCCGAACGAAATAAAGGGTTATTCTGGCAACGAGATTGTCTCCTCACCGAGCAAAGTCAGT TTGATGAGTGCCCAGAGCTATGGTTCCCGCTGGACCAACCAGTTTGTTACCTCCACGGGTCGCCTTCGCGGGGCCGTGGT TCGTATTAAGGAGTTGAAATTCCCGCGGAAGCGAGACATATCCAGGGAGATCATGAAGGAGATGCGATTGTTGCGCGAAC TGCGTCACGATAACATCAACAGCTTCATTGGCGCCAGCGTGGAGCCAACACGCATTCTTCTAGTCACCGATTACTGTGCC AAAGGCAGTCTTTACGACATCATTGAGAACGAGGACATTAAGTTGGACGATCTATTCATCGCCTCGCTTATTCACGACCT CATAAAGGGCATGATCTATATACACAACTCTCAGCTCGTCTATCATGGTAACCTTAAATCCTCAAACTGTGTGGTCACCT CGCGTTGGATGCTCCAAGTCACCGATTTCGGGCTGCACGAGTTGCGGCAGTGTGCCGAAAACGAATCCATCGGGGAGCAT CAGCATTATCGAAATCAACTTTGGCGCGCTCCAGAGCTGCTGCGGAATCACATTCACGGCAGCCAGAAGGGCGATGTCTA TGCCTTTGCCATCATCATGTACGAGATATTTAGTCGTAAGGGTCCGTTCGGACAAATTAACTTTGAACCAAAGGAAATCG TAGACTATGTCAAGAAGCTGCCGCTCAAGGGAGAGGATCCCTTTCGGCCGGAAGTGGAGTCCATTATAGAGGCCGAGTCC TGTCCGGATTACGTACTGGCCTGCATCAGGGACTGCTGGGCTGAAGATCCCGAAGAGCGGCCCGAATTCAGTGTCATACG CAATCGCTTGAAGAAGATGCGTGGTGGTAAGACCAAGAACATCATGGATCAAATGATGGAAATGATGGAGAAGTATGCCA ACAACTTGGAGGACATTGTCACCGAGCGCACGCGTCTCCTGTGCGAGGAAAAGATGAAAACGGAGGATCTCCTGCATCGC ATGCTACCGCAATCGGTGGCTGAAAAATTGACCATGGGTCAGGGTGTCGAGCCCGTTTCATATGATTTGGTAACCATATA TTTTAGCGACATTGTTGGTTTTACTGCAATGTCGGCGGAGAGCACACCGCTGCAAGTGGTGAACTTTTTGAATGATCTCT ACACAGTGTTCGATCGCATCATACGCGGCTATGATGTCTATAAAGTTGAAACCATTGGAGATGCTTACATGGTGGTTTCG GGTCTGCCAATTAAGAACGGAGATCGACATGCAGGCGAGATAGCTTCGATGGCCCTGGAGCTGCTCCACGCTGTCAAGCA GCACCGCATAGCCCATCGTCCCAACGAGACGCTGAAGCTGCGCATTGGAATGCACACGGGTCCGGTGGTAGCGGGCGTGG TTGGTCTCACCATGCCGAGATACTGCCTTTTTGGTGACACCGTGAATACAGCGTCCCGGATGGAGAGCAACGGAGAGGCG CTAAAGATTCACATTTCCAATAAGTGCAAGCTGGCGCTGGACAAACTGGGTGGTGGCTACATAACCGAGAAACGTGGCCT GGTCAATATGAAGGGCAAGGGCGATGTGGTTACTTGGTGGCTAACCGGAGCCAACGAGAATGCTATTCAGAAGAAGTTAG TGGATATGATGGACATGCCGCCGCCGCTGTTTAGTCGACCTCGAAAGAGTCCAAAGCTAAATCCCGACTCCCGTCAGCCA AGCATCCAGGCCATGCACTTCTGCGGCACCGGATCAAGACGCCAGAGCACAGTTCCCAGGGCGATGGACGGCGAATCAAC GTATAGCCTCCAGGGATCCGTGAGGGAATCACCGCGCATGGTCAGCAAACGTGACAGGGATCGAGAGCGACCCCCCATCA ATGGACTGGGCGCAGGGCACTTCGTGGGGGGAGCTCTCCTCGAATCCGCACAGGCCTCGCTTAGCACGCTGAATCATTCC GAAACAAACGAAACTAACTGTGATATGGATGGGGGATCGGGAGGAGTGTCAGGATCTGGATCTGGATTAGTGCGTCAACC CAATGCACTGCACAAACCGTTGGCCATGGTGCGGCCGCACCGGATTATTAGTGCTGCCCAGCTACCCCAACTGGGAGATA ACGATGATGACTCCGCAGACACGCTGTTGCGGGAGTCTCGCTCCCTGGATCCCATGCCCATGCAGCAACTGCGCAAACGT CACGACAGGGTAAAGTTGCCGCCCTCGAAGTTGTCCAAGAACAATTCCCGATCGCTGGACACGGGTGTATCGCTGATCAG TGGGAATCCCAACGGTGAAGTGCACTCGAGTCAACTGGATTTGGATAACGAGATGACCGCCAACCCGGTGGATGCCACCG ATGGCTACGACGATGAGCTGGGCCTTCTGATGCGCCACGACAACGGCCAACTGCCTGCTCTCCGCTATTCAGGCAGCTTT CCCAACGCTCAGATTAGCATTGTTCCTACAGGAAGGTCAGCAGGAGGAGGAGGAGGAGGAAGAGAAGGAGGTGGGAGCAA CTGTGCCAAGCATCTGAACAACAACTGCAACGGAGGAGTGAACGTCGAGGATGATCTTGAGTCGCCCCTGCTGCAACGCC AGGCATCGCTGTCGGTGCCGCCAGAGGAGATGCTGGCGCACAACAAACGTTGGCACTCACTGGAGCACATGGATGGTCCC GGTGGGCATGGCGGAAATAGCGTTAGCTATGCCGCCGATATTGATAACCGCCACCCCGGAGATCTGGACTTCTTCAGCGG TTCCTCCAATCAGCATCATAGATCCAAAGCGGCTGGTGGGAGTAAGCTGACTAACTGGATGACTAACATCTTCAAGGGCA ACGGCGTGCGTAGCGGTGAGGCTAGGCGAGTAGGAATCCTGCCCAGCGGTGTGCACGGCGCACGAACCGGATTCACGGAT ATGGCGGCATCGGCAGCAGCCCGCGATCGTGAGAGTATAGTATAGAGATAAGGAGAAGGCAGTGTAGAAACTAAGGAAGA AGGAGCAGGATGACCAGGATGAGCTAAATAGCCGGATGAGAGAGATGGAAAAGTTACTCAGGAACCGAGTCGAGCAGGAA ACGATCGTCTTTTGGCTTACTTCTTTGTTTTATGTATTGTAGTTTGTATGGATCGAACGAATTGTGTGTCTGTGTTGGAA GTCATCCACCTATAAACATATACCAGCCGGTTCTTAACTCTTTGATCGCTCTGGCATTGCGGGACTCTACATATGTGTCC TATGGGTTTCCAAACTATTTTTATTCGTTTTCGAGGAAAATCCTGCGATCCCGAAGTAGAAGCGAGCAGAAGCTGAAGCG AAAATCATACATAGCATATATTTTATAACTCTACATACATACATATTAGAGAAGCCGAAAAAACCGTCTAGTCACAAGGT CTTCAGACTAGTTATTGTTTTATATAAAATAAACACACACCCACACATCTAAATGTACGTTTGTAAATGTAACACATAAA CACAACGACACACATATTTATACGACGATGGAGAATCAAAATCAAATATTTTATGTAAATGTAATCGAAATTTTCTGCTT TCCATACACGATGCGCAGCAGCAAACAAATTATGCTTAAACAGAAACTAGTTAATTTATGTATTTGAAAATCAAAACGAG CGTGAAAAATGTAATGTATCAATGTGTTTTGCTAGTACGTAGTTAAGTGCGTGTGATTGTACGTAATATTTAAGTCTCTA AAGCTAAAATAATAATTATAATTAATTGAACGCGAATTGTGAGCAGAGAAGAGCGAAAATGCGCCCGATGAGAGGTGTTT TAGCTGTATGTATGTATGTATGTATATTTTTAAATGAAAATTTGTTAAATTTGCCATATATAAATACAAACGAGAAACTA TTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.