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FB2026_03
,
released September 17, 2026
Comment: 0-22 hr AEL
AGAGAGAGAGAGAACTAGGAAGTGGTTTTTCCACCGAAAATATGGACAAAGGCAAACTGCAGAGTTAGCTGAAAATAAAA AAAAATAAAAAAAAGGAACAAGACACAAACAGTGACTGAAATAGCGGTTAACAGCGCTCCCTTACGGCGAATCGTTGAAA TTCAAGACGGAGAGCTATCGCATTTCTTCAATTATGTCTGTTTCGCTTTTCATTCCGCTGCAGTAAGCGATCGCGCGAAG GAGCAGCAATACAGAGAGCGAGATTTGGAGAGAGAGAGAGAGAGATAGAGAGTGCGCTCCCAATTGACGACTCCACTCCA CTCCCACACACACTTAACCCCGCCCCCTCGCACCCGTAATTACTATGGATTGTGGTGGTATTGCGGCACATCAGCAACAA CAACTACAACAGCAACAACTGCACTTACTCCAGCAACAGCTACAACAACAACACCACCAGCAGCAGCGGCAACAACAACA GCACAGCCTCGAGGAAGCAGCAACAATAACAAATATCAACCTAAGGGGCATCTTGGTCGGCGGCGCTGCCAGCAGCGCAG GAGTAGCAGGAGCAGCGGCTGGTGGTGCGACGACGACGACAACGACGACGACCGCCGCTGGCTTCGTGGCTGGTGGAGAT TGCCCTGTGATCGGCGCCGGTGCTGGCGCTGGCGTCAAGCTGCGCAGGCACAGCGATCGTCCCCTGACGCCGGAGGCCAA GCAGCTCTCTCACACATACCGCATCTCGATGGCCAACCTGGAGGATTCGCAGGAATCGGAGCTGGACCAGATCCTGGGCG AGCTGAGTCTGCTGGAGGCGCAAATTAGCTACACGGAGGCCTCCATGCTGCCCGCCATGTGTGCTCCAAGTGCGGGCGCT CAGATAGCACCGCCACCGGGAGTGACTCAGCTGCCGGGCAGTGCTCCAACGATGGTCTCCATGTCGGCATCATCCTCGCG TTCCCATTCGCGCACCAATAGCACCATCTCAGCAGACGTAAGCAGCTGCTCGTCGTCGGGGATCTCGGAGAATGGACACG GACTGGGACTGGTACTTGGCGGGCCCGGGTCGGCTGGAATGATGGTGCAGCCACCGCCACCGGGCGGCATGACCATGGGA ATAACGCTGGGTGTTGTCACTCCCCGGGAACCGCGCACCGAATCGCCAGACAATGATTCCGCTTTCAGCGACACAGTTTC GCTGCTGTCCAGCGAGTCGTCGGCCTCCTCGAACACATCCCTGCAACAACAGCAGCAGCAGCAGCAACAACAGCACCACC AGCACCAGCAGCACCACCAGCACCAGCAGCAGCAGCAGAAACCACAGCTGGCTGGTGCGGAGAAACTACACCACCACGGC GTACATGGGCAACACGGTGGCCAGCAGAGTGGCGCCAACAGCATCACGAAGGCGGACAAGATCCAGTTGGCACTGCATAA GCTGGAAAGCGCCCCCATCCGTCGGCTGTTTGTCAAGGCCTTCACCTCCGACGGTGCCTCCAAATCACTGCTGGTTGATG AGCGAATGGGCTGTGGCCATGTAACACGACTTTTGGCCGACAAGAACCACGTGCAAATGCAGTCCAACTGGGCGTTGGTC GAGCACTTGGGCGATCTGCAGATGGAACGCCTCTTTGAGGACCACGAGCTGCTGGTGGATAACCTGATGACGTGGCACTC AGACGCCGGCAACCGAGTGCTCTTTCAACAGCGTCCGGACAAGGTGACGCTGTTCCTGCGGCCGGAGCTCTACTTACCCG GACCCCAGATGGCGCCCGGATGCCAGCACGACGAGCAGACGCGGCAGATGCTGCTCGACGAGTTCTTCGATTCGCACAAC CAGCTGCAGATGGACGGACCGCTTTACATGAAGGCGGACCCAAAGAAGGGCTGGAAGCGATATCATTTCGTGTTGCGCTC CTCGGGCCTCTACTACTTCCCCAAGGAGAAGACCAAGAACACGCGCGACCTGGCCTGCCTGAATCTGTTTCACGGCCACA ACGTGTACACTGGATTGGGATGGCGCAAGAAGTGGAAGTCGCCGACTGACTACACCTTTGGCTTCAAGGCGGTGGGGGAC TCTTCGCTGGGCAAGTCGTGTCGCTCCCTCAAAATGCTGTGCGCCGAAGACCTGCCGACGTTGGACCGCTGGCTGACGGC CATCCGCGTCTGCAAGTACGGCAAGCAGTTGTGGGACAGTCACAAATCGCTGCTCGAGGATCTCTGCCTGAGCCGCGACG ATGCCGTCTCGCAGTCGAGCTTTGCAGCCTCCATGCGCAGCGAGTCCATCTCGAGCATCTCCTCGGCGGTTCCGTCGCAG TGCGGCAGCGTTTCCTCGGCCATCAGCAGCATGTCCAACTCGACTAGCGGCCGCACATCGCGCGCCTCTAGCAGCAGCTC CAGTGGCTGCCTCTCGGATGACAACAACGCTTTCGACTCCGAGTTCACCACTGGCACCATTAAGCGCAAGCCCTCGATGA AGCCCAATCTCCCGCTGACCACAATGACACGCCAGCTGAAGGAGGTGGGCGAAATCACCATCTGCGAGAGCGCTGGTGGC GATGCCAGTTCCCCGGAGCGCAGTGGTACACTAACGCGGCGCCATAGTCGCCGCAAGTCGCAGGAGAGCAACGGCAGCGG CACGCTAAAGCGCCGCCCGATTGCCGTGCCGGTGGCCACTGTTGTCAAGCAGACGGAGCCAATGGGCAGTGCCTCGTCAA CGTCGTCCAGCAGCAACTCTACGCCCACGCCCACACCGAGCATATGCGCCAAACCGCCGCCAGGCGATTCGGCATCGCTG ATGTGTTCGTCTACCCTGTCTTTGGATTCGCTGCCTCCGCCCCCTCCGCCGCCCGCTTTAGATGGTTCGGAGGATCAGGA TGTGTACGGGTCACAGCTGTCGCTGGCCTCACTGCCACCACCGCCTCCGCCTGAGGATGTGCTGGCAATGAACTATGCAG AACCCTCCAGTCCGTCTACGCCCACACCAATGAGCACTCCAATGATCATGCCCAACAGCAACGGATCGTTGCCCCCGGCA GTGCCGGCCAAACCCATGAAGCCAGCAGTGAAGCAGGCGGCGGGCGGTCTCAAGGCCGCGCCACCATACAAGGCGCCGCC GGATTACGTGGGCCCTGCCCTGCTGCCCGGGCCGCCATTGCCGCCGCCGCCTCCTGCCCAGAAAAAAGTCTCGTTTGCAG ACTCTCCGGTGCTGTTGCGTCGCAAGATGTGCTCACCGGAACCCGTGCTGCCCCAGCGATCGCCCAGCACCACGCTCAGC TGTCATTCCAGCTCCAGTGCAGGATCGGCCTATCAGACCTACGCGCCCGGGCCTATGTTGCCGCCGCGTGCTGATGTGGC GCGCCTGTCCAGCCTGAGCAACGGCAGCAGCAGCGAGGTGACCTCGCCCAAGCGCCTGCAGGAGTCGGCCTCGAATCCGC CCCGCGACTTTCTCAAGGATCTGCAACGCGTGATGCGCAAAAAATGGCAGGTGGCTCAAAAGTGCAAGGCGGAGCCAGCG ACGACGCCTCATGAGGTCCTGGGCTTCCGGGACTTTAGCAACGAAGACCTACTGGCTGCCCACAACCTCAACTCTGGGGC CAACTCCTCGCACTACTACCGCGAGACGGCCAACGTGAGCCACTGGGTGAGGAAGCACTACGAGTATGCCCACAATGCGC TCTACGAGAACGTGCACGCCCAGTCGGCGGCGGCGGCGGGAGTGCCTGGTAGTGGAGAAGCCACCACGCCTCCGCTCCCA CCTCCGCCTGGTAATTCAGTGGCAGCTAAAAAACGTCCGCCGCCGCCGCCTCCGAAGCGGAGCGACAAGACGCACCTGAC CAACCGGGTGTAATGGCATCGGAGAAGAAGAGCGAAACCGCTTACTTACTGAGATCCTCGTTCTCCAAAGGAAGGATTTT CGTACCAGAATCAGAATCAGACAGTTATGCACTTGTCTGAAAGAAAGCAGAGAAAAGAATCTTACAAATTCCTATTTAAG TCCAGGCGACAGCAGAGCGAGATGGAAAACCACTAATTAAAACCTACATATATATTTCCTAAGAATATGCTATGGCCACG CCCACTTCTCTCACAAAAGTATTACACATCATAGAGAATCTAACTGTTGAACTTCTTCGCAGTTCGGGATTAAACCACGT CAAAAGTGTCAACAAGAGAGTTTTTAAATTTCCAAGGGCAGGCGTCCAAAAAGAACTTTTTGTATATCTAAATTAAAAAC CTCTTCTTGTATATTTTTAAAAACCTTTAATTTTATGAACGAATACCTTAACAAGTCAATAGTTTCGAGCATCTCCCATT ACATATTTTTGTTGGCACTCTTGACTTGGTTTGATTCATAATCTATATTTTCATTTTGAAAATCAAAAAACTTCTGCGGT ACTCGCCTGCGGAATTAAATGCAAAATGCAAAATAGGAATTCTGGCATTAGTAAAAATATTTAAGATACATTTTAGTTTG TAAAAAGGAAAAATGCATTTTAGAAACGATAGTGCATTATTTAAGCAACACTAAATTATCGCTAGATAAGTGTACAATGT TTTTATATATACATGCACTCTTTTAATTGTTTAGTTGCTCGTAGTATTGATTTTATACATTATATCTTTTTTTTGCGATT ATCTTTGTAACGGTAGAGTGAACGTTTTGTGAGCGCATGAAATGCGCTGCGCTAGCAAAAGGAAATGCCTTATAAAGCAT ATTAACCTAAAAAGGGGAAGCCCTGCATAAACACCTAATATACATACATAATTACACACATAGGCCAACATACCTATGTT GATCTATCCAAATGATTTGCTGTTTAAAGCGATACTATTCTCCTTTTCGAAATTTGTAATACCACTTTGATTGTTCAGTT TATGAAACCAAATGCTTAGTTTCAAAAGATTTGTCAAGTTCCTCATTAGTGTTAAAGTTGTATTTCTCTTTGTGCTTGGA AAAGCAGACAGATCAGCCATAGAATGCAATTTGTATGTGCAAATCAAACTATTTATATTGAAGGCAATAAGAGAAAATGG AACTAATTCTGTACACAAATCAAATATGTATTCATATAGTAATTGTAATTGTAATAATATATAAATTAATATATTTGGCT AAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.