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FB2026_03
,
released September 17, 2026
Comment: 0-22 hr AEL
CATTTTTTACTCGTCGTCGCGTTTTTTGGGCTTTCGGGTGTATTTTTTTGTTTTGAATTGTTGACACATCTCCGGGACGT TTCATCGTTTTGGATTGTAATTAGGCGTGCAAGTGGAAAAGATAATAAATCAAAGCCAATGTGCAGCGTGAGAGTAACGA AAAAAAAAACAAAACAATCGCAGCGTGAGACTTTGCTTTTTGTTGTCTTTGCTAAGTGATGCTGCAGCAACAATAGTAAC CAAAGCATCAAGAACATCAATAAGAGCTCCCGCAAAAATTCGCCGAACCTAAACTTATTGCAAAACAACAAGAATTGTCG TAATACGGTTGTTTTTTCTGCATTATTACTTCATAACAGAGTGTCGCTCTCTCGCTCGCTCCGGAAAAGCTCCGCTTTTG GGGTTCTCTCTCGCTCAGAAAACAGCCAGAACTTTTAATCAGTCCCAGAATGCAGTCCAAAGAGCTCGCATGCGGTGCAA AGTCAATACCGCTCTAACTGTGCCAGGACCATGTCGATGATTCGGATACCAACCAGGACTATGGCGTCATTTGTATGCGG TGTGGCTATTCTGCGACAACTAAGGGAAAGGAAATGAGGAGCCGCGGCTAATCCAAGAATCGCACAGAAAGTAAACAGAA AGCGTTCATCAGTGGGTCAGTAGCAGCCCGTAAACGAGATTTTAAACCCAAAAATTTGTGAGGATGATGAGCGCCGGAGG AACCTACATCTCCACCGCCAGCGTGGAGGTGCCACAAGCTCTGCAAGATGGCGAGAAGTTCATTCGATGGGATGATGACT CGGGCACTGGAACACCGGTTACAATGCGCGTCGATGCCAAGGGCTTCTTCCTCTACTGGGTCGACCAGAACAACGAGCTG GATATCCTTGATATAGCCACCATACGCGACGTTCGCACAGGACAGTACGCTAAGCGACCAAAGGACAACAAGCTGCGCCA GATTGTGACGCTGGGCCCCCAGGACACGCTCGAGGAGAAGACAGTGACGGTATGCCACGGCTCGGACTTCGTCAACATGA CGTTCGTCAACTTCTGCTGCACCCGGCGGGACATAGCCCAGCTCTGGACCGACGGACTCATCAAGTTGGCCTACAGCTTG GCCCAGCTCAATGGCTCGGCGATTATGTTCCTGCAGAAGGCCCATACCAAACTGTGTCTCCAGGTGGACAAAAGCGGCCG CATACCAGTGAAAAACATCATAAAACTTTTTGCCCAAAACAAGGAGGATCGCAAACGTGTCGAGAAGGCGCTGGACGTGA CCGGTTTGCCGTCGGGAAAGGTCGATAGCATATCGGTGTCCAAATTTCAATTCGAGGACTTTTACAATTTGTACAAATAT CTCACGCAGCGTTCGGAGGTGGAACGCCTCTTTGACAGCATCGTTGGCAACTCGAAGCGCAAGTGCATGTCCATCGCTCA ACTGGTGGAGTTCCTGAACAAGACACAGCGTGATCCCCGCCTGAACGAGATCCTCTACCCTTATGCGAATCCCGCCCGCG CCAAGGAGCTCATCCAGCAATATGAGCCCAACAAGTTCAATGCGCAAAAGGGCCAACTCAGCCTGGATGGCTTTTTGAGG TACTTAATGGGCGACGACAATCCCATCATGGCGCCCAGCAAGCTCGATCTCTGCGATGACATGGACCAGCCGATGTCGCA CTACTTCATCAACTCCTCGCATAACACCTACTTGACGGGACACCAGCTGACCGGCAAGTCGTCCGTCGAGATTTACAGGC AGTGTCTCCTGGCGGGATGCAGATGCGTTGAGCTCGACTTCTGGAACGGACGCACCGAGGAGCCAGTCATTGTCCACGGC TATACGTTCGTGCCCGAGATATTTGCCAAGGACGTGCTGGAGGCCATCGCAGAGAGTGCATTTAAAACATCCGAATACCC TGTGATATTGAGCTTTGAGAATCACTGCAACCCTAGGCAACAGGCGAAAATTGCCAACTATTGTCGCGAAATATTTGGCG ATATGCTGCTCGATAAGCCTCTGGACTCTCATCCGCTGGAGCCGAACATGGACTTGCCACCGCCGGCGATGCTGCGCCGC AAGATTATAATCAAGAACAAGAAGAAGCATCACCACCACCATCACCATCATCACCACAAAAAGCCAGCGCAGGTGGGCAC GCCGGCAGCCAACAACAAACTGACTACAGCGAATTCAGTGGATGCCAAGGCGGCGCAACAAGTCGGTTTGTCGGCCTCGC ATGAGGATGGGGGCGTGACCAGGAGCACCGCCAACGGTGACGTTGCCACTGGAACAGGAACCGGAAGTGCTGCTGGGACA GCAGGACACGCGCCGCCGTTGCAACAAATCCGCCAAAGCTCCAAAGACAGCACTGGATCCTCCGATTCGGACAGCTCTTC GGAGGATGAATCCCTGCCTAACACCACGCCCAATTTGCCCAGCGGCAACGAACCTCCGCCAGAAAAGGCTCAAAAGGAAA CGGAGGCGGGAGCAGAGATTTCAGCTTTGGTCAACTATGTTCAGCCCATCCATTTTAGCTCTTTTGAGAATGCGGAAAAA AAGAATCGATGCTACGAAATGTCTTCGTTTGATGAAAAACAGGCGACAACATTATTGAAGGAGCGACCGATTGAATTTGT GAACTACAACAAACACCAATTATCTCGTGTCTATCCGGCGGGCACTCGCTTTGATAGCTCCAACTTTATGCCGCAGTTGT TCTGGAATGCCGGATGTCAGCTCGTGGCACTTAACTTCCAAACCCTCGATCTGGCCATGCAACTCAACTTGGGGATCTTT GAGTATAACGCTCGGTCCGGTTATCTACTTAAGCCGGAGTTTATGCGCCGATCAGATCGCAGATTGGATCCCTTCGCGGA AAGCACTGTGGATGGCATCATAGCAGGCACAGTATCAATCACGGTTCTATCTGGTCAGTTTCTCACGGATAAGAGGGCGA ACACTTTTGTGGAAGTTGATATGTACGGTTTGCCAGCGGACACGGTGAGAAAGAAGTTTCGCACCAAGACAGTGCGGGAT AATGGAATGAATCCCCTTTATGACGAGGAGCCGTTCGTGTTTAAGAAGGTGGTTCTCCCCGAGTTGGCGAGCATTCGAAT TGCAGCATATGAAGAGGGTGGCAAACTCATTGGTCACCGCGTTTTGCCCGTAATCGGTCTTTGCCCTGGTTACCGACATG TGAATCTCCGATCTGAAGTGGGTCAACCAATAGCCCTTGCCTCTCTTTTCCTGTGCGTGGTGGTCAAGGATTATGTACCC GATGATCTTTCCAATTTCGCAGAGGCGCTAGCTAACCCTATTAAATACCAAAGTGAGCTAGAGAAGCGCGATATTCAACT ATCTGTTTTGACCGACGAAGCCGAAGCGCTGGGCAGTGCGGACGAGGATCTCTCCAAGTCGTTCGTTTTCGTCCAAGTAG GTGGCCAAAAAAAGGAGCTGCGTCCGGTTGAATCGCTTGCGACTTCGCCGAAGCATAGGCCGAGCATCTCGGCCGCCGCA GCAATGAGTGTTGACGTTACCGTGGATCGGACCGATGGCGGAAGGGGAGAAGATAGCATCTCTATTGTAGCACCCTCAAT ACAGCATCAGCACTCTCTAGACCAGTCCGTAAGCACCTCCATCCGACAGGTGGAGTCCTCGCAGTTTGATGTCGATCTTG TGTTGGCGGAGCCGCTGGAAAAGATATTGGATCACAAGTCCGTCAAGGAAAAGCGTCTCGAGATGGAAAAGAAGCTGGAG TCACTGCGAAAGAAGCATGACAAGGAAAAGATTAAGATCGCCGGCCAGAAATCCAGTCCGCTAGAGGGCAAGAAGCCGAA GTTTGCCATCACGAATAAGCTGGTGAAGCGGCTAAGCAACAAGAGTCTCGAGCCTGGTGTAGAGATCCCCGCCTGCCCTC TGGATCTGGGCGATAGCAGCGAGGAGAGTGCAGCCGCGGATGCCGGTGAGGACTTAGCCGGTGGGAGCAGCAGTCTGGAT GGCAGAACTCAAGAGTCGCGATTACGCAGTGCCTGCCGGGAGTACACTTCCCAGTACCGTGAGCTGCAGGAGAAGTACCA TGAGGCAATTTACAGTGCAGCCGAGAAGGTGCTGAAGACATCGCAAACTGGGCAAACAAAGCAGTTAAAAGCATCTCTGG ACAAGGTCACTGGAGAGGTTATGCACCAGCTGCAGGAGGCCCGCCGCAACGAGGTTAAGAATCTGGCCACCGTGCACCGG GATCGTGATGAGCTGATCAGAATGAAACGCGAGGTGGCAAGCTCCGTTGTGGAACGTGGCGTAGCTGAACGTGTGCGGCT GAAGCAGACCTTTGACCGGAGAACGGATGAACTTCAGAAGCAGCACGACTCAGTGCGCAATGCCCTGGCAGAGCACCGCT CCAAGGCTCGTCAAATTCTCGACAAGGAGGCAGAATCGCGAAGCTGTGTATCCAGCAATGGCTTCTTGGTCCTCTTCCAT GGACCTCATCACCATGGCTGTACGGGCTCCGGCTCGTCAGCCCTTTCAGGCAATAATCTCACTCTAAATCTGGACGCGGG GGCTGCCGGTAGTCATTCAGCCATTTCGCCGGCAAAGTCGCACAACAGCATCGCAGCAGCGGCCGAGATGAAGACGTAAA TGGGCCACGCCCGTATAGGTTTTTCTCCCCGCCCAAATTATCCCTAGGATTAATTGTGGTTCTAAAGTTGTTAGTCTTCA GTATTTACAGCAGAAACATAGGAATGAAAAGTCTTCGTAGTATATAAGCAGTGGAGGAGTAGTCCCTATCCTCCCACACC TATTTGACGGGAACTGACCTCGATTAGATCTTAGGACCTTTGACCCTAGGCATTGTAAATCCCACTGCTAAGGGGAATCC AAGCAAACACGTTTTCTAACTGAACAAACTAGATGTAAATCTATCGCATGTATGATGTACTTGTAGTGTAACTGTTGACG TATCCGTACTTTACAGTACTTTATCTGTATACTGAACGGTGTATTTCTATGGTGTTAATGGAGGTTGATCCATCACACTC CCGTCAAACCGTACGGTTTCTTCCTATTCGAACTTCGCTGAAACTCTGAGTGTCTAATTAAGCAAGCAAATGTAACAGAA GCAGAAAATAAGTATAATTTATTTGTCTATACGAAGGAGACTGAGTGCTCCATTTGTCTAATGCTAATTGTGGTTGTGTG TATTTTACATCAGTTTTAATCGATATAATTTATACAATTAGTTTAAGCCTCTCACACAAACTGTGGATTGAATCTCGTGC GCACACAAGGCGGATGCTAGAGCGGATGCCAAAGCACAAGTCTAGGCCAACAATTAGTCATTTATTTCTCGGTTGGTTGC TATTTATTCTTGATTTAGTCACTATTCTTACGTTTACCTAGTCGTTACAAAGCTCCAATAAAATTCTATACGGATTGTTT TTCCCCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.