Comment: 0-22 hr AEL
TCGCGTCGGCATTTAAACAAAATCCGCGGCATTTCGGTAGTTGCGATTAATAATTGCTTGGCGGGAATTACAGTCGAATA AGAAATTGGCGAGGAAAAACCAACAAATGTGCAATTGCTAACGTGATCGGGCCAAGGCACAAAAACATTCCATGCATCAT CAGCAGCGCCTGCGGGCAAATGGCGGACGAGGAGGCACTGGATTGGGCGCAGGATCAGGACCTGTGTCCGGAGGACACTC ACCATTGTGCGCCCATTGTCGGGGTCAACTACTGCCACATCCAGAGGAGCCAATCGTGATGGCTCTCGGCCAGCAATGGC ACTGCGATTGCTTTCGCTGCTCCGTTTGCGAGGGCCATCTGCATAACTGGTACTTCGAACGGGAGGGCTTACTGTACTGT CGTGAGGATTACTACGGCCGATTCGGCGATGCCTGTCAGCAGTGTATGGCCGTTATCACCGGACCCGTAATGGTCGCCGG CGAGCACAAATTCCATCCGGAGTGCTTCTGCTGCACGGCATGCGGCTCCTTCATCGGCGAGGGCGAATCGTACGCCCTTG TCGAGCGTTCGAAGCTCTATTGTGGCCAGTGCTATGGCAAACGTTCCTGCCAGCCGGCGGATGCGAAGGCGCGGATCACA ACGGCCGGCAAGCCGATGCACTCCATCCGTCTGGTCGAGATACCCAAGGACGCCACGCCCGGCCTGAGGGTCGACGGAGT GGCCCTCGACGATGGTTGTCCCACGGTCCGCATTACAGAAATTGATGTGAATCTTACAAATCTGCATATTGGAGATAGGA TATTGGAGGTGAACGGCACACCAGTTAGTGATTCGTCCGTGGAACAGATCGACAAATTGATACGGAGTAATGAGAAGATG CTTCAGCTGACCGTGGAGCATGATCCGGTGCAAGTGTGCCGCTCCTGCAGCCAAGCGGACATCCAGCGGGCCATGTCAGC GTCCACGTTAATCCTGCCGCTCAGCACATCGGCCTCCAGTGTCGAGGTGGGCAGGGAGCGTCTGTACAAGACACCCGGCG AACAGGGCACGAAGGCCAGGAAACTGCGACAAGCCACCAATGCCTCTACGACAATACCACCGGCAGCTGGAGCCACCGCA ATGACGCAACTAAAGGAGAAGGAGCGTTGTTCCAGTCTGTCCAAGTTGCTGGATGAACAGCATCAGGCACAACAGCATTC GGCGCATCCTCAGCTCTATGATCTGTCCAGGACGCAATCCTGCCGTGTCGTCCAAAAGCCACAACGAATCTTTCGTGCAA CTGATCTGGTTATAGGAGAGAAGCTAGGCGAGGGCTTCTTTGGCAAGGTTTTCAAGGTGACCCACCGTCAGAGCGGCGAG GTGATGGTCCTCAAAGAACTGCATCGTGCGGATGAGGAAGCGCAAAGGAATTTCATCAAGGAAGTGGCCGTTCTCCGTTT GCTGGACCATCGGCATGTGCTCAAATTCATTGGTGTTCTGTACAAGGATAAGAAACTGCACATGGTCACGGAGTACGTGG CCGGTGGTTGTCTCAAGGAGCTGATCCACGATCCAGCCCAAGTACTCCCTTGGCCCCAGCGCGTCCGCTTGGCCAGGGAT ATTGCGTGCGGCATGAGCTATCTGCACTCGATGAACATCATCCATCGCGATTTGAACTCAATGAATTGCCTGGTCCGTGA AGATCGCTCGGTCATAGTGGCGGATTTTGGACTAGCTCGCAGTGTGGATGCGCCGCGGCTGCCAAGTGGCAATATGACGC CAGGCGGCTATGGATCAGGCGCTAACAGCGATGCGCCCATGTCACCGAGTGGAACACTAAGGCGCAGCAAGAGCAGGCAG CGAAGGCAACGTTACACGGTGGTCGGGAATCCCTACTGGATGGCACCCGAGATGATGAAAGGTCTCAAATACGACGAAAA GGTGGATGTCTTCTCCTTTGGCATTATGTTGTGTGAGATCATTGGACGCGTGGAAGCCGATCCGGATTTTATGCCTCGCA ATTCGGACTTCAGTCTCAATCAGCAGGAGTTTCGCGAGAAGTTCTGCGCTCAGTGTCCAGAGCCTTTCGTTAAAGTGGCC TTCGTGTGCTGTGATCTTAATCCCGATATGCGTCCGTGTTTCGAAACTCTGCACGTTTGGCTGCAGCGTCTCGCCGATGA TCTGGCCGCCGATCGTGTGCCGCCAGAGCGTCTGCTGCACGAGATTGAGACCTTTCAGGAATGGTACGCCAGCTCTGAGG ACGCCTTGTCACCCACCTCCCAGCGCAGCTTAAATAATCTCGATGAGTTGGTCAAGAGTGCGGTGGATTCGGAAATTTCG CCGGTAGAAAAAGAGAAGGAGAATATGGTGATTAAGCCCCAGGATATACCGAAATCTCCGCATCTCGGCAAGGATTTCTC GCCCAGCGGCGAGAGACTGCGTGACAGTATGCGGGCCAGGCGTCGCCAACGTTTCTTGGGCGCCCAGGAGGAGCGTCGTA ATCTCACGCCCGACACGGAGTCCAAGGAAAGGGCTTTGAAGAAGGCGCTGAAGAAATGTCGTCCGTTTGGCGAAAGAGGT TACCTGGTGGATCTGCGTGCCGGTGCCGAATTGCAGCTAGAGGATGTCAGGGATTTGAACACCTATTCCGATGTGGACTC CAGTTGCGATACTAGCCTAAATTACCATGATGTAAATAATCTGCCAGCAGCGCAAGAGGATGAGAATACAGTGAAACCGG GCAAGGAAGAACTGTTGGAGGAGAGCACAAATAAACCCAGCAACCAGGAGTCACAACACCATCGTCTGGCCATCGATGAT ATGCGCACCCGCCTGAATCAGTGCCGCAGCAAATTCGAGCATTTGGAGGAGGCTAGTCGGCGGAACTTCAACCAGTCGCA GCACTCCATGAAGAACTTTTTCAAAACCCCACCGGTGGCCCTTAAGATGTTTCAGCGCCTGGAGCACGAGGCTGCAGCCC TGAATGGTGGCAACAATTGCCCACCGCCACCACCACGTACGCAGCGCATCAATCAGACGCCCATTTTCGGTCGAAAGAAT CCGCCAGTGGCAATCGTCGGGCAAAAACTACAGCATGCTGAGTCCCTAGAGGACCTGGCCTCCAGCGGAGTTGCCAAACA GCTGGCCACACCAGCACCCAAACGCAGCAAAGCCACAGCAACGACCAAAGGCGGCCAGAGCAGCAATCCGCCACTTTTCC TGCCGCCTAGCCTTAACATAAGTGTCGCATTAAATAGCAATGGAAATGTGACCACAACGACCAATACCAATAGTAGTTGT CCACCATCCGCATCAGACTGGCTGCCGAAGAAGCACAAGCTAACTTTGCCACTTCCGTCGGCCCAACAGCAGCGAACGAG CAGCAATCATCGCCTGCCAATGTGCAATAACAAAGGGAAGACACTGAAGCCACTGCCAAGTCGCACGGGCAGTCAAGGCA TTCCCGCAAGCAATTGCGTCTCGCCGACGAGGAGCAGCAGGCCCGGATCACCCACCAAGCATCTGGCCCAGAGGCACACA GCCGCCACGGCGCAGCGACTGACAAATGCGGCCGCCACTCACCAGCAGCAGCATCAGCAACAATCCTCGAAGACGACGCG CCTGAATATCCTGAGTCCGGAAAAGGTGCATCGCCTGGGCGCTCGACTCACCGATCAGAAGCAGAAAATGCGCGAGGAGG CCGCAGCGACGGCCTCGTCCGTCGGAGGAGCCGGTTGTGCGGCAGGAACAGCGGCTGGAAGCTTAAATGGTCATCGGACC ATCGGATCCTCCGGCACACCGAATTCGGCGGTCGGTGAACGGAGGAGGCGAGCAGCTCCATCGCCGCCAGTTCGCACGCA TTTCAATACGCGCTGCTAGCTAGACGAAGTAGCCATACTCTCTATTAACGATTGTCAAGTGTTGTGCGCAGTTTGTTGTG GTTCCACTCAGGAAGCTGTTGTAAGCAGCTTAATGTTCTACAAACAAACTGTCGTAATTAGCGTAAATCTTTGAAGGATA TAACTGTTGTAACTTCTATAACCAACCATTCGCCATTTGTATCGTACGGAATTAAATTTCCACGACATGTAGAGGCACTT TTTTTTTTAAATACATAATGACTTTTAAAGTGTAATGTAACATTAAGAACCAGTGAACTGTGCCGCTGTTATAAACGCAA CTGTTATACGCACATAACAAGATCAACATTCCGACGAAAGTGTTTATTTTTTTTTTGATTCCAACATTTGATTTTAAGTT TCAACTGAACGAAGGTTAAGTGCCTGATAGGAATGAAAAACCAATAAGTTGCAATATTTACACGGATTGTATAAACCAAG CGAATAATTATAGTATGTAAGATTACAGGGTAACAACTGTCTCGACTAACCTAAAAAAAAAAACTGAACTAGAAGCAAAG CTAAGCAAATACTTGTTTAACCCACTATTGTCTGACATAAAGTTATATTGCGAAAGAGATAGCGAAAGACGGATAAAGAG AGAAGGAAGAAACTTGTGCCATGATGTTGATGCGAAAAACGAACGAATCTGAAGAATCCTCGAGAGAGGGAGATCCCACC CGCAAAAAAAAGACAAAAGGAACGAAAAGAACTTTAACTAGAAAAATTGTGGTTTAAATATTTTTTGATAAACAGCGTAT ATAAATGTATTTGTATATCTATTCCATAATTATATAGATAAACGTATTCCATGTTAGTGCTTAAATCTAATTTATTATTT ATCGATTCTCGACCCATTCACCCCGTCCCCTCATTCCAAGAACAATAATTAACAATGCTAAGAAACAAACTTTTTTGTAT TGACGAGAATAAGTCTAACTCCTATAACTAAATATATAAGAATGTTTTTGCTCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
