Comment: 0-22 hr AEL
CACACAAACCGAGTTGGGATTTCGTTGTAATTGCTGGTCATTTTTCACGGCTGTGCGACTAATTAAATTTAACAAGAGAG CCTTAATAAATAAGTGCTAAAGACCAGACGCAGCGCGCAGTAATTCAAATCATCTAGAAGATCTCGTAAAACAAAATTGA TATTCAGAAAGAACTCCAAACGCTACGTGGCAGAGGCGAATCACCGGCGCAGCAACACCAGCAGCAGATAACCAGCCGTC AGTCCAATTTCTCTACCTTAGAACTCGAGCGCATTTGCCGCTTGCGGGGCGACCAAATGCATAGACAACATATGTCGCAT TCACGAGCAGTCCAAGATCAAGAGTTGCGACCCCGACCGTTTGTTGCATATTACATACGCTCATAGAGGGCGGCCACTCA CCTAGAGCAGCCAGAGTCCTTGCACTTGGGCAGGGATCCAGGGAGCAACAAAGTGCTTAGAAAGAAGAAGCAGCAGCACA GGATTATACGCTCCGCCGAGGACAGAGCAACAAAAAAGATGATGAAACGCACTCGCGTAGACGAGGTGCAATTTGGCACA CGACCCGTTCCTCAGACCTCCGGAGGTGTGGGCGTTGGTGTCGTCGGCGTAACTGGAGGTGGGCCAACATCCGGCGGCGG TGGCACTGCTACTGTGGGCGTCAATACGACGGGCGTTACCATTGGTACCGTCGTGCCCAGTGCTCATAACGCCACCATCA GCGGGATCGGTTCCATTCACCATCGCATCCTTACTCCACAGCATGGAGGAGCACAAACCATTGCCTATTTGCCGTCCACC ACGCCGACGGCTACGAATTTGAAGACCACAACCTCGATTGTGGATAGCACTACCGCTGGAGGACCAGTAGGAGCAGGAGC ACAGGTGGCCGTAGGAGTTGGCTCTGCAGCCGGCGGAGGAGGCGTGGTGGTCTCCACAGGGAGCACGGGAACGCAAACAT TACAGTATACAACATCTTATAGTGTGGCTTCGATACAGGCTGGTGGAACTCTGAAAGCCAACACAGCGGATGGCGCGAAC ACGGTACAGATTCATGTCACAGGAGGAGGAGCTGCAAACAATCCTGCCAGTGCACAGACTGTTTCCAGCAGCTCACAAAC GGGCACAATCCGTCAGCGTACAATCAGCGGCACCCAGACAGTGGCCACTGCAGTGGGTAATCTAGCCACGATTTCCCAGC AACAACCGGTTCAACAATCACCTTTGGGCAAGGCCCAAACGCCTCCATCGTCCGTGGTTGCCAACAGTATCCCAGTGGGT GGAACCACCCCACCACAAGGACAGTCGGGAAATGCCACGCCACGCTTAAAAGTGGAGGATGCGTTGAGCTACCTGGACCA AGTAAAGTACCAGTATGCAGACCAGCCGCAAATCTACAACAACTTCCTCGACATAATGAAGGAATTCAAGTCGCACTGCA TCGACACACCCGGAGTGATTGAGCGAGTCTCCACACTCTTCAAGGGGCACACAGAGCTGATATACGGCTTCAACATGTTC TTACCGCCGGGTTACAAGATCGAAATTCATTCGGATGCTCTTGGCTGTTCGGTGCCGGTGGTTTCGATGCCCTCACCACC GGGAGCGCCAACGAGCACAGGAACGGTGCATATGCTCACCGGAAATAGTTCGATGTCCGGTGCCGGACATATCGCCATCA AGACGACCAATGCTGCGACTTTAACACCGGCAGCAGGGGCTGGAGCAGCGGCCGCAGCAGCAGCTGTGGCTCAGATTCAA TCCGCTGGAGCTGTAAATCTAATGACCCACGGCGGAGCCTCGCTTACCCAGACCACTATTCATGCATTGCAACAGGCGAC GCCTCCTCAATCACAATCTCCAGGCGGAGGTCATGTCCACGTGAGTGTAACAAACTCTACGGCAGCAAACGCCGTAGTGC CCGGACAGCCAGGCATCTCCGTATCGGCGCACAATGTACCGCAGAACTACTCGAGAGATCGAGAAAGGGCCACCATAACA CCTACTGGGCAAGTGGCTGGAGCAGCGGCAAACGTGAATGCGTCTGCTAGTATAGTTGTCGGTGGACCTCCAACGCCCAA TTCTCTAAGTGAGCTTAGCCCTCATGGAGGAGCAGGAGGTGGGCCAGGTGCGGGAGCGGCGCAGCACAATCTGCATCACA TCCAGCAGGCGCATCAATCGATTTTGCTTGGGGAGACGGGACAGCAGAATCAGCCGGTGGAGTTCAATCACGCCATAACC TATGTAAATAAGATCAAGAATCGTTTCCAAAACCAGCCGGCCAAGTACAAGAAATTCCTCGAAATTCTGCACGATTATCA GAGGGAGCAAAAGGTGATGAAGGAGGGCAGCCTGAACCAGGGTAAAATGCTCACCGAGCAGGAGGTATACACGCAGGTGG CTAAGCTTTTTGGCCAGGATGAGGATTTGCTTAGAGAGTTTGGCCAGTTTCTTCCTGATGCCACCAACCACCAGTCCGGG CAGTACATGTCCAAGTCAGCGTCTGTGCACAATGACCATGGCAAGCGTCCCACGGCTACTTTAAGCGGTGGAGCTCACAT AACCATGTCATCTGCTTCGCCAGCGCCAAGTGGATCCCCTCTGCATTTGGGTGCAACGACTCTTCCGCAGATCGACAAAA GCGCCCATGCAGCGGCCATAGGAAACCTTTCGGCGGTGAACACTAGTGTCAGCATTAAGACGTACAATAATAATCAGCAA CAGCAGAATCATGTGATCGGTTCAGGGCTGAATGCGACGCGGAACGATATTCTCTTTGAGAAGGACTATCACGCGGGTCT GCAGCAGCAAGCGCATCAGCGAGGAGCTGGAGTTGGAGGCCATCATCATTTGGCCGGGACTGCAGCGGGCGCCAATATCG GACGGCCTGGAGTGGGAGCCAGCGTGATGGTGTCGTACGATAAAGAGCATCGAAATAATCATCATGTACAAAAGTACGTT GGCCACGCGCCAAACCAGAATCTAACGCATGGTCATAACGCAAAGAAATCACCAAGTTATGGCATTCCTTCGGTGATTGG TTCCATGCCGCACATTTCGGACAATTCGCTCGACCGCAGTTCACCGGGAATTAGTTACGCCACGCCACCATTGCCCTCTG GTCCACATGGACAGCATAACTCTGGTTCAGCTACGAGGAGGCCGGGTGATGACAGTTTAGTCGGACACTATGCTAGTGGA GCGCCGCCTGCTAAGCGACCGAAGCCTTATTGTCGTGATGTCAGCTTTTCCGAAGCATCGAGCAAGTGCACCATCTCCGA CGCCGCGTTCTTTGACAAGGTGCGAAAGGCGTTGAGAAGTCCAGAGGTCTACGACAACTTCCTGCGATGCCTGACCCTTT TTAACCAGGAAATTGTCTCCAAAACGGAACTTCTTGGTCTGGTATCACCGTTCTTGATGAAGTTCCCCGACCTGCTGAGA TGGTTCACCGACTTTCTGGGACCGCCCTCTGGTCAACCTGCTGGAGGATTGATTGACGGCATGCCCTTAGCCGCAACGCA ACGTCAGGGAGGTGGAAGCAGTAATAGTTCCCATGATCGAGGTACCAGTCACCAGAGTGCCGCCGAGTATGTCCAGGATG TGGATCTGTCTTCTTGCAAGCGGCTGGGTGCATCCTATTGTGCTTTGCCGCAGTCCACAGTACCTAAGAAGTGCAGCGGA CGGACGGCTCTCTGCCGGGAAGTGCTCAACGACAAATGGGTATCGTTCCCGACGTGGGCCAGCGAGGACTCCACTTTTGT TACATCGCGAAAAACTCAGTTCGAGGAGACAATTTACAGAACGGAGGACGAGCGATTCGAGCTGGACCTGGTCATCGAGG TTAATAGTGCCACGATTCGAGTGCTGGAGAACTTACAGAAGAAAATGTCACGAATGTCCACCGAGGAATTGAGCAAATTC CATCTGGACGATCATCTGGGCGGCACATCTCAAACGATACACCAGCGGGCCATTCATCGCATCTACGGCGACAAGTCGGG AGAGATAATCACGGGAATGAAGAAAAATCCGTTTGTGGCGGTGCCTATTGTACTGAAGCGACTGAAAGTCAAGGAGGAGG AGTGGCGAGAAGCGCAAAAGACCTTCAATAAGCAATGGCGGGAACAGAACGAGAAGTACTACCTCAAGTCTCTCGATCAC CAGGCCATCAACTTTAAGCCCAACGACATGAAAGCCCTGCGCTCAAAGAGTCTGTTTAACGAAATCGAGACGCTGTACGA TGAGCGGCACGACCAAGAGGACGACGCCATGGAACCGTTTGGGCCGCATCTGGTGCTGCCGTACAAGGATAAAACCATTC TGGACGATGCCGCCAACCTGCTGATTCATCATGTGAAGCGTCAGACTGGCATCCAGAAGCAGGAGAAGCAAAAGATTAAG CAGATCATCCGACAATTTGTGCCTGACCTTTTCTTCGCGCCGCGACAGCCACTAAGCGATGACGAACGCGATGACGCCTT TCCATTTTTGGTAGATGACAATACGAAAATGGACGTGGACTCGCCACTGGGTCGCACGGAGTCTTCGACTCGAAATGCCA AGAGTACGCCCAGTGAAAGTGCCTCTCCGGCGCGTAGCAATGCAAGCACTAGCAGCGTAACACCAGCGGGAATTAAGAAA GAGACTGATGACTCGAAAGCGACGACAGGATCTTTTGCACCAGCGTCCTCGGCAACGGCATCTTCAGCGACGCCTGTGGA CGATGCTACGCCATCAACATCCTCAGCAGCGGCGGCAGCATCAGCGGCATCGTCATCAACTGTTTCAGGAACCGAAGGCA AGCCAAAGGATGACCCCCTATCGTCGCACAAGGAAGAAGGAGCCGGTAGCACCAGCTCTGGAGTTGCCACGAGTCCCAGG CAAGCCCAAGACACTGCAGGAGCAGGCGTCGATGTGGAAATCAAACTGGAACATCCTGCGGACTTTTCCAATCCAAAGCT TCTACCGCCGCACGCGCACGGGCAACGCGAAGACGAATCCTACACGCTGTTCTTCGCTAACAACAATTGGTATTTATTCC TGCGGCTACATGCGATTCTCTGCGACCGTCTGCATGTTATGTATGAAAGAGCGCGTTTGCTGGCCATCGAGGAGGAGCGC TGTAGGGTGAATCGAAGGGAGAGCACAGCCACGGCGCTGCGGCTAAAGCCCAAACCGGAGATTCAAGTTGAAGACTACTA CCCCACCTTCCTTGATATGCTCAAGAACGTCCTAGACGGCAACATGGACTCGAATACGTTCGAGGACACGATGCGTGAAA TGTTTGGCATTTATGCCTACATCTCTTTCACGCTGGATAAGGTTGTCTCCAATGCTGTTCGCCAGCTGCAATATTGTGTC ACAGAACGAGCCGCACTGGATTGTGTAGAGCTGTTTGCAACGGAGCAAAGGCGTGGCTGCACCGGAGGATTCTGTCGAGA TGCGCACAAGACATTTGACAGGGAGATGTCGTATCAGCGGAAAGCTGAGAGCATTCTCAACGAGGAGAATTGCTTCAAAG TGTACATTTACAAAATCGATTGCCGTGTAACCATAGAGCTGCTTGATTCGGAGCCCGAGGAAGTCGATAAGCCGGCTGCG TTGAAGGCCCAGAAGTTCAGCAAGTATGTAGAGCGGTTGGCGAATCCTGCGCTCGGCGGTGGTGGCAATACTGGCCGCTC CGATTCGGCGCTCGGCAATGACAGCGTTGTAGATGGGTCCGATATCAAGACGGAGGCGGACGAAGATACTGCCGAGTTGC GCTACAGAGAAGGTGGAATCGGAGGAGCAGGCAAGGCGCGCTTTTTGGTGCGCAACAAGCGGAGATCAAAGCTGCTTGAG GAGCAAGTGCGTCAGTTGTTTGAACATAGGCGGAATCGGATTGAGCAGCATGGTACTGCGGCCTCACTTGAACCCATCTC CGTTGACAGTGCCATTTCAAGCAACAGCACCACCGGCGGTGGCGGCAGCGGCACTAATAACAACAATAATAATAATAATA ATAACAACAGCTGGGGCGGCAAACGTCTGGAGAGGCTGGGCGCCCCACAGGTGGGCTTGGCCGAGCAATACGCCTTTAAT GATCGCGACGAGATCAGCACAAATATCAGCAACGGGCGATGTTTTGTGACCAGCAAGAATCTCAAGCTACTTAAGTACGA TGCAGTGCGTCGTGCCAAGAAGAGTCATTGCCGCGTAACTCAGGCGAAATACGCTCACTTCCAAACGTACGTTAACAAGT GGCTTCAGCAACATGTCAGCGAGCAACAGCAGCAGAACTGCGTCGACTGGCTGCTGGGCAAGACGGCAGACCAAATCAAT GCCAGCGGCTGGGGATCCGCCAGCAAAACCAAGACGGTACAGCAGAAGGATACTTCGAAGACACCGTATCGCATCTACAA CAGATACAGAGTGGTGCAGAGTGCAGTAAGCGGCGGTTTGATGAGTGCCACTGCTCCAACTCCAGATGGTTCAGTTCCGG CGGTCAATTCCGCGCCATCAGGCTCAGTTTCAACGGTGAACAATGTACAGGGCTCGGCGGCAGTATCCTCAACTTCCCAC TCGATGTGCAACAGTAGCATTTTGTCCGCGGACTCCACCACCAATACCAATGCGTCAGCACAGCAGCAGCAGCATTGCAA CAGTGCAGCAGCTGCAACAACACCGAATGCAGAGGCACTGCAACAGGTGCGACCCATGGAGAGCTAACCGACGGGAATGG GAGCAACAACGGCAGTGGTCGCTGCATGGCCAGATTCTTCATTCACATGACGACAATGAGAAGATGTGGGATGAATTGCC TGCATTGAAATAAATAGTGTTTTAAATGTGATACCAGTTCCTCGACGCGGTCATCGAGATTGCAGCTATACCAACGCGCG ATCGACTGTTTAATGTCCGGCATGGCATTGGGCTCTGGAAATTGATTGATGAACGAAGGAAAGCTCTTTTTCATATTGTA CAAAAAGTCATTTAATCTTAGGCGAAAGTGCTCCTAAATAAGAATTTTATAGTCTTACGTTGCATAAATTTTAGAATTAA TTTTACGACGGCAGCGTCCTGTTTTATTGATGCGCTTTTAGAATTAAGATATAAAGTTTATACGCCTAGAGCCACATACA CCTTAAACACAATCGCAAGACCACAATTTAAGTCACGGATATTTTTAACGATTTTGCACTAGAAACCGATCGAGACAGTT GTGCTAGTAACTAGGAACCAGAACACGTCTGTTTAGTGTTTTAAGATTTTTTAAAACCAAGCTCTTTTACTTTATCATAA GTGTAACGTATTTTTAATACAAAACAAACAAAACACCCACATACAAACCGTGTACAATTCCTTGTGAATACTATTGTTTA TATATTTTACGCGTTTAAGGTTATTAAGTTAAATTGTAAAAATGTATTGCGCAGATATTAAAACAAAAGTTTATCAAAAG TGAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
