Comment: 0-22 hr AEL
AGCGCGAAGTGCGTGCGTGTGCGTGCGTCAAAAACGCAGTTCTGTATGTGTGTTCGTGTGCTTTTCTTATATTTTTCTAA AGTAATGTTATTGAACTGGTTGCCTATGTAAAGAGAAAGCCACGAAAACTACAAGCATTTGTAAATTCGTTGAAAAATTT GCAGCTTACCAATTCCAAAATCTCAAAACAGTGACTGCAACACCAACAAAAACGCAGTAGCAGCGACGTCGGCAGAGAAA CAACAAGAACAACAATCTTGGCGTTGAAGCGATGTGAGAGAGTGTAATATTTGACAGCATGTGAGCTAATCAGCAAAGCA GCGATTTCTGCAAACATTTTGAGCTTCTTAAGGGCAATTAATGAACGAAAAAGTGGAAAACTATAGTAAATATATAGTAA GAAATATAGTGAGAAATTTTGTATTTTTACGTTTTGTTCTCTAGAAATTAAAAAAAAGAGAGGATACAAAAAATTTAGCT GTGACCACCAAGAAACGCAGTCGACTTCGCTGCCGGCAGAACGAAGAAAAAGAAAGAGCATAATATAAAGCAGAAAAACC AGATTTTAATGTCTAAATTTTAAAGATTAAATGTTTTATTTTGCCTTATGTTTGCCCATTTATACTCAAAAATATCGGTG TCAAAATAGTTGCAAATTCAGAACAGCAAAGCAACAACAAAGGCTGCTGCTTGTACGACCGATGCACAAATCTTGTCGGG AAAAATAGGGTTCTGAAAGCGAAATTATGTTTTTATTGGCTTAAAGATGGAAAATTGACTCCTGAAATCGAAAATCAAAG AAACTAATACGCAAAGTAATCAAATAGTGACAATTAAGGCAAGATGACGGACTTTGTTGAGCTGATGAACAGCATGTCCA GTACATTCAATAGCGACTGTGCTACTTCAACAGCGGAGGGTGGTACTTTGCTAAATCTGAACTTGGCGGAAGACAAAACC CTCAAGTGGCGTAATTTGGCCAATAACCAGTTCGCTAGTAAAGAAAAGAAGCACAAGGACAAGGAGGAGGAGGAGCGCAA GGAGGCAAGGAATCAAGAGGAAATCGAAGACATCAAAGCACTTTTGGCAGATGTTGTCGATGCGGCCGCTGTCAAGCTGG AAGAAGAAGAAGCGCAGAATGCAGAAAAAGTCGAACCACATACAAAATGCGAAATAGAGGAAGAAGGTAGGAAGGAAATG GAGTACGATCAAGATGTCGCAAAACAAGACTCCGAAATGGAAAAAAAGCAGAATGGCAAAGCAACATCCATAACAGTAAA GATGGAAAGCAACGAGCGTGCAGAGAAACACGCAACGGAAATTGCAACGACGTCGACAGAGAGATGGGAAAACGAAAGCT TTAAAACTGAGCAGCAAAATAAAAAAGCTGCTGAGAAAGAAGAAGAACCCATACTAGCAGCAACACAAAAATTGGAAGCA AATGCAGAACCACTGACAACAACAAGAATTGAGGTAGCAGTAGCATCTCCACTTGTTGTTAGTTCTGCTAGCGTTAAACT CGCTGCTGACGCAACCAACCAAATGCGAGCAGCGACGTCGGCAGGAGCAGCGACATTAGCTGATAAAAATGTACAAGTTT CGCCCGGTGGAACGCGACGGTCTCGAAGAACACCCAGACCCATAGACACGCCCACTTCAGTTACTGATGAACATGTTCAG GTGGAGAATAAAAAATTTGGAAAGTCGGAGCAGTATACGGATTGTAGTAGCCATCTGGAGAGGTTCACACTGGACGATAA TACTGCTATTGTCAGACTGCAGTTGAAAAGTGAACCGGACAAACCGTCATTAACTGCATTATCTCCTGAGGAAAACTCGG CTCCTGCTCCGAAGCGAGGAAGGGGTCGAGCCAGGAAAATAAGACCCGATGCCGAGGTGGAAACCAGTGAGGTTATTTTA CCCTGCGAGGATTCATTAGGGGAGAAGAAACCAGGACGCAAAAGGAAACTCCCGGATGAACCGATAGATCAGCAGCAACT AAGCGACCTTGTCGTGGTAAAAACAGAGCAGGAGGAATTGGGTGACGCTCCCTTGGGAGATGTTAAGAGAATGCGACGTA GCGTTCGTTTAGGCAATCGATTACACGCCGATGGAAGCCCTTGGGAGGAGGTAAAGACAGAGGCACTTCACCCGCAGCCT TCTGCAGAACTTTCCTTTGCGGAGGTCACTTCTGAGATCTTGCCACTAGCTGTCTTAGATGAGAAGACCCCACCCAAAAA GCGAGGGCGGAAGGCAAAAACGCCCTGTGTGAAGTTAGAATCTGAAACATCCTGCGGGCTACCGTTTGCTAATGGCAACA AGAAAACGAATTCCTCTGGTGGATGCGAACTTCAGCTGCCCAAGCGTTCAAAAAGAAGAATAAAACCAACGCCAAAGATA TTAGAAAATGACGAACTGCGCTGTGAGTTCGAAACGAAGCACATTGAGCGTATGACCCAGTGGGAGAGTGCTGCAGCAGT AGATGGGGATTTTGAAACGCCAACAACAGGCGGTAATGGGTCCAATTCCTCGACCTCAAGGCAAAAGAGCGACAAGTCCG ACGGGAGCAACTTCGAAGGTGGTCCAGGACATCCTGCAGGCACAAGTGCCATAAAGAAAAGACTATTTTCAAAGTCCCAG CGGGACATTGAGAACTATGGCGCAGCAATGCTAGCCAAAAGTAAGTTACCTCCGTGTCCGGATGTCGAACAATTTCTAAA CGACATCAAGGCATCTCGAATTAACGCGAATCGGTCACCAGAAGAAAGGAAATTGAACAAAAAACAGCAACGAAAACTGG CCAAACAAAAGGAAAAGCACCTGAAGCATTTGGGCCTGCAAAAAAATCACAGAGATGAGCCCTCGGATAACGACAGTTCC AATACGGACAATGAATTCTTTCCTACTACAAGGGTGCAAGTGGGAAAACCAAGTGTTACTTTGAGAGTAAGGAATAGTGT TACCAAGGAGCTTCCCACCACAGCCACTTTGAAATCACGCCGGAATCCAGTCGTGCAGGCAGCCAAACTCACGAGAAGGA TAGGTGCAAGAGCCGCCGGTGAAGTTACTGAGGCAGCCCGAGCAAGTGTGCCAATTTCTACACCTGATGCGGAGCAGCTT CACTCGTTGGACACTTCTATTCAAGCTGATGTTACGCCCATAAGAGACTTAGATATGAGGCCCAGCACCTCCAGAGTGTC AAAGTTTATTTGTCTATGTCAGAAGCCTTCCCAGTATTATGCCAGGAATGCCCCGGATTCATCATACTGCTGTGCCATCG ATCACATCGACGACCAAAAAATTGGCTGCTGCAACGAGCTGTCCTCAGAGGTTCACAATCTACTCAGACCCAGCCAACGC GTTAGCTATATGATCCTATGCGATGAGCACAAAAAGCGCCTACAATCACACAACTGCTGCGCAGGATGTGGCATATTTTG TACACAGGGAAAATTTGTGCTATGCAAGCAGCAGCACTTCTTTCATCCCGATTGTGCCCAGCGATTTATTCTAAGCACTT CGTACGAGAAGGAACTCGGCGATGAGGAAGATCAGGGCGTCAAATTCAGCAGCCCTGTCTTAGTGCTTAAGTGCCCCCAC TGCGGATTAGATACACCAGAACGGACCTCCACGGTGACCATGAAGTGTCAGTCCCTGCCTGTCTTTCTGCGCACGCAAAA GTACAAGATCAAACCCGCTAGATTGACAACCTCTTCGCATCTAACCCAATTTGGAACAGTTGAGAATGCCAATACCCCAG GAGCCACCGCCAGGAACAAGGGAGGACTATCCACCGCCGTAACCTTAAGCGCGGCATCAAGTCCTGCCTCAAAAACAAAT GGAGCCCAGAGAGGCAGGGCGGGCACCTCCAATTCTAACTCCAGGCATGCTCTTAATTCGATCAACTTTGCTCAACTTAT ACCCGAGTCCGTAATGAATGTAGTGCTGCGGGGCCACGTGGTTTCTGCTAGCGGTCGCGTGACCGCAGAGTTTACCCCGC GGGATATGTACTATGCAGTCCAAAACGATGACTTGGAGCGTGTAGCGGAGATTCTGGCTGCCGACTTCAACGTGCTGACT CCCATACGGGAGTACTTAAATGGCACCTGCCTTCATTTGGTGGCCCACTCGGGCACACTTCAGATGGCCTATCTCCTTCT CTGCAAAGGAGCAAGCTCACCGGATTTCGTTAATATTGTGGATTATGAACTGCGGACTGCTTTAATGTGTGCCGTGATGA ACGAGAAGTGCGACATGCTGAATCTATTTCTTCAATGTGGTGCGGATGTGGCCATTAAGGGTCCCGATGGTAAGACCAGT CTGCATATTGCTGCCCAGTTAGGCAACTTGGAAGCCACACAGTTAATTGTAGACAGCTATCGAACTTCTCGAAATATTAC CAGTTTTTTAAGTTTCATTGATGCCCAGGATGAAGGCGGTTGGACGGCCATGGTCTGGGCAGCAGAGCTGGGGCACACGG ATATAGTTAGCTTCCTGCTTAACCAGGATGCTGATCCAAACATATGCGACAATGACAACAATACGGTGCTGCACTGGTCT ACGCTGCACAATGATGGTCTGGACACGATTACAGTACTGCTGCAGTCAGGGGCGGACTGCAATGTACAGAATGTAGAGGG CGACACGCCTTTGCACATTGCTTGCCGGCATTCTGTCACCCGCATGTGCATCGCGTTAATAGCTAATGGAGCCGATCTAA TGATTAAGAACAAGGCGGAACAACTGCCCTTTGATTGCATTCCGAATGAGGAGTCTGAGTGCGGTCGCACTGTAGGTTTT AATATGCAAATGCGTAGTTTCAGGCCTTTGGGCCTCCGTACCTTTGTGGTTTGTGCAGATGCGTCGAATGGCAGGGAGGC ACGACCAATCCAGGTGGTTCGCAACGAACTGGCAATGTCTGAAAATGAGGATGAGGCCGATTCGCTTATGTGGCCGGATT TTCGCTACGTAACACAATGCATTATCCAGCAGAATTCAGTGCAGATTGACCGACGCGTTTCTCAAATGCGCATCTGCTCC TGCCTGGATAGTTGCAGTAGCGATCGCTGTCAGTGCAATGGAGCCTCCTCGCAGAATTGGTACACGGCAGAAAGTCGGCT AAACGCCGACTTTAACTACGAGGATCCTGCGGTAATCTTCGAATGCAACGACGTCTGCGGTTGCAACCAGCTGTCCTGCA AAAACCGCGTGGTGCAGAATGGTACACGGACACCCCTACAAATTGTCGAGTGCGAGGATCAGGCGAAGGGCTGGGGAGTA CGGGCGCTGGCCAATGTACCCAAAGGCACTTTTGTGGGCAGTTATACGGGTGAGATTTTGACCGCGATGGAGGCAGATCG ACGCACCGATGACAGCTACTACTTTGACCTGGACAACGGGCACTGCATCGATGCCAACTATTATGGAAATGTAACCCGGT TTTTTAACCACTCGTGTGAGCCGAATGTTTTGCCCGTTCGTGTTTTCTACGAGCATCAGGACTACAGATTCCCTAAGATC GCCTTTTTTTCCTGCCGTGACATCGATGCCGGAGAGGAAATATGCTTTGACTACGGAGAAAAATTCTGGCGCGTGGAACA TCGCTCCTGTGTTGGATGTCGATGCCTGACAACAACCTGCAAGTACGCCTCCCAATCCTCAAGCACAAATGCCTCGCCTA CGAATGCAACAACGGCACCGGAAAATGAAACGGGAACGCTGTCGTCTACAAATACGGAGAAAATTGGACACGCGTAGGCT AAAACCACCTAAACCTGAGATGATTGCAGATTTCACAGTCTCATCCAACAAATAAAATCCCCACCTAAACCATCAGCAAC CAGATGAGCCGGGCATATAAAATCGCAGCAACCCCAAATTCGATAACAATATTTGTAATATAAACATTGAATCGTTATCG AAGCCCGGTCGGCTTTCTTTCTTTTTTGACTGCCAAATGTTTCGTGGTTAACCACTTTTTATCTATTTTTACGTATACAA ATAGTGTTTGTTTATTATTAATTTTGTTTGATTTGTATTAATGTTTTACGCCTTATTAATAATGTTTTTATTCTTAGTAC CTGTAAAAACAAATTAGTTTATACTTTCTGCGCGAAACAAACTAATGACATAAGAAGGGCATATCAAAATTGTTTCCCGA TATTGTGGGTGTGCGTGTTTCCGGAACGTAAATGTTTTAAACTTAAAATTAGACTTGGACATTACCAGGCATAAAATAGA ATTGTAATGGAGGAAAGACCATCTAGTCATAAGAATGAAGGAGACAAAGTATTTCCATAGAAAAGAGAAAAAAAATTTTT TTTAATAGAAGTAGTCGGTTTTTTTTTTTAATTTGTATGAGCTCTGGAGCATACATATGTAAATACGTGATACGAAAAAG TAATACGTTTGATTTTACCGTTAACCATGAAAATTAGACCGTTTAAATTGACCTGTAATGGGCAACTAAATGTTACTCGC AACCAAAAGTAAAGGAGGAATTATTTAGCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
