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FB2026_02
,
released June 18, 2026
Comment: 0-22 hr AEL
CGATAGTTCTAGCCATCTTCTATCGCCTGTTGGTCGTCGGTGAATTTATTGCGAATAGAAACTAAGCTAGCACTATAGAT GCCGCATTAGCAGCATTCCAGGCTGTGCCAGTACTTGGATCACATATTAAGCAGCCAGAAAAGCCGTACAAGAGATGACC AGGACGTGAGACCGGCGAACCGGCCCACCATTTTCCACGTACTTGTTGTTGTTGCTCGGCACTGCACATTGAGACCCGAC GGAATATACATATGTTTTCTGGTCAAAAAAAGAGCACCGAATCGCTGTGACGACTGCACAAGTAGCTGCTTCTTTACATA CGCAATTCGCCGGTGGAGTTGGTTTCGATGATCGGTCACTGAGACCGCTGTCCAGAAGATTGGTCGTCCAGACAGACAGA AGAGCCATCCGCGAACATGCTACACGTCCTGTTTTCCGTTCTCCTCATGGCCACGGCCAACACGGCCGCGGACACCCTCC AGCGCAGCCGGCGAGTAGCAATCGACGTGGATAACGCAAAAGACATGCACAGCATTATCGACTTGGCCGAGTGCCAGGAT CTAAAGAAACTCTGCGCGCACCACAAGTCCACCGACAATCTGTCCATGCTGGAGTGCGCCCTGACATTCAGCTCTAGCCA GTTGATGGATCTCCCGGAGGGTTGCCAGAACGCTCTGTGGATGCAGCAGCGCCAGTTGCAGTTGCCCGCTTGGTTAGAGA GCACTTTCCTGCCCGCTTATTGTCCCGCCGAGCGGCCCAAGCTGGAGAGCTGCCTGAACGCAGTGCACCTGTGGAGCTGC CTGGAGCAGCAGCGATTAAAGCTACTGCAGAACAACGCCTGCCATCAGCACCTTCGAAGGGCCTACGAGTCCTTGGGGCA GGACTACAGCGCCGTAGACGAGTTCTACACCGCCTGCGGACCACTCGTGGAGGAAAACAAGTGTGGCCGACTGAATGTCG ACCACCTGCCTTCCGTGCTGTCGCAGCTGACAACGGTTCAATGCCTACAGGAGAGTGCCACCAAGACCGGCATCGAGCCA GTTTGCCAGGCAGCCATCAATTCTATTGAGCTCTTTCGCGTCTGCCAGGAGGATTTTTCGTCCTTTTGCTCGCAGGAAAA GTCCGGCACTGCTGCCGGGTACAAGTGCCTCGTGCGGCACAAGAACCACCCCTCCATGTCTTCCCAGTGCTCCGCACGAA TCACTTTGAGGGACCAGCAAATAGGCCGCGACTACCGCGTGTCCCACGGTCTGGCTAAGGCCTGTAAGGACGACATTAAG TTGTACCACTGCCGGCGCGGCGTCTCTGAAGACAAGCACGTGCGGTTGGCTCAGATCCTGCTATGCCTGGAATCAGTGTC CAAAAACGGGACTAAACTGGCGCCCCCTTGTCTCACGGAGTTAACAGATCACCGCCGCATGCTGATGACCGACTACCAGC TGTCACCGGAGCTACTCAACGACTGTGCGGATGACATACCCAAGTTCTGTCCGGACGAGCACAAGGCGCAGCTGGTGAAT GGCATGACAAGTACAGGTGGCGAGATCATCCACTGCCTGTTAGAGCACGTTAAGGCTAGGCGTCCGCAAAGACGGGTGAC GGCACAATGTCAGCGAGGACTGGAGACACTGATTAAGGCCAGCGATGCTGGAGAGGATTGGCGAGTCGATCCGGTGCTTA GACGCGCATGCAAACCAGTGGTGGATGTGGTCTGCAGGGATGTGCAGGGCGGTGAAGCACGAGTCATGGGTTGTTTGATG GAGCACATTGGCACTCCGGTTATGCTGCCCGATTGCGAGCAGGCCCTTCTCATTATTGAGTATTTCGTTGCCAGAGATTT CAAGTTGGATCCCCAGCTGTACAAACACTGCCGCGATGACGCTGTGAAGTACTGCCGTGCAAAGAAGCAATGGGATGATG CGCAGAACATACAAATGGATCCGGAAAGGGGACCCATGATCCTGCCCTGCCTACATCGCATGGCCTTCAGCGAGGACGAG CACCAAACTTTGAGGAAGGACTGTTTTCAGGAGGTGAAGCGAGTGATGAGACAACGCGCAATATCCATGGACTTGATTCC TGAGGTAGAGGATTACTGTTTGAACGATCTTTCTGCGTTCTGCGCGGACTGCACAGAGAAGGGAAGTGAAATGGAGTGTC TGCAAAAGAACATGGACCAACTGCAGCCGGAGTGCAAGACAGTTGTGGTCAAGTATACGGAGGAGGAGGCAGCCCACGTC GAGCTCAACCCCGTCATAATGAACGTTTGCGGAGAGGCCATGCAGCAGCACTGCTCGGCGATCCTCAAAAGCGGAAAAGA CAATGGCGACATGATGGACTGTCTTATAGCGCACAAGAACGACGCAGATCTGCGCAAGGATCTCCGCTGTCGGGCTGCCA TCGAGCACTTCCAGATCATTTCGCTAAAGAGCTTCCACTTCACTACAAAGTTCAAGGAGGCGTGCCGTCCGTATGTCCAG CGCTTTTGCTCCTCCAGTGCCACCAAAAACGAAGTGGTAGCTTGCCTCAGTGAGGTCATGCGCAACGACACAATCAAGGC GCAGCGTCACCAGATCCCCAAGGAGTGCCGCCACCAGGTGAAGGCACAACTCTACCAGCAGCGCGAGAGCATTCAACTGG ACCCCAAGCTGGCCAACGCCTGTAAGCGTGAACTGGAGCAATTCTGCGAGGAGGAAAAGGGGCCCGGTCAGGCTCTGGAG TGCCTTATAAGAAAGACCCATAGTTTGGGGAAGCCGTGTCATCATGCGATCTTCATGGTAAAGAAGTCTGAGTTGGGCGA CAGTGGCACCGACTATACGCTTCTCACCACGTGCAAGGAAATGATCTACAAGTTCTGCCCCAGCACGGATTCGTCAAAAC TACTGGACTGCCTCAAGACGTACAAGGATGACACGCAGTTCGACCAGCGGTGTCACCTGGTGGTTGTTAATCGCATGATC GAGCAAAACACCGACTTTCGATTCAATCCCTCACTGCAAAGCGCCTGTGGAAAGAACATCGATCGGTATTGCTCCAACAT TGTGGCCAGCGCTTTGCCTAATGAGGAACTAAACGGAAAGGTGATTCACTGTCTAAAAGACAAGTTCCGCCAATCTGCGC TGGACGAACCATGTGCGAAGGAGATGATCAAGATCCTGCAGGAGCAGGCTCTCAACTACAAGTTAAACCCCCTGCTGCAG GTGTTCTGCAAGTCGGAGATCCAGGAACTGTGCAAGGCAAATGTGGATTCCGACGAGCACGGCCAGCTGGCGGAGTGCTT GAAGACGGCCTTCCTGCAGAAGCAGATCATCAACCGGCAGTGCCAGATGGAGGTGGCCACTCTGATCGCCGAAGCCAAGG CAGACATTCACGTTGATCCCATTCTTGAAACGGCGTGCACGGTCGATCTGCTGAGATACTGTTCCAAAGTGGCAGCTGGC AATGGACGCAAACTTAATTGCTTAAGGACGCTGCTGAAGGATACGCCGAACTCGTTAGAGGCGGACTGCCGAGAAAAGCT GCAGCGACGCATAGAGATGTTCAGGAACGCAGACGACACGTTGGCGCTTCCGCCCGAGGATGTGCAGCAGCTAGTGCAAC AAGTCGTCGCCTCTCCGGCGCGGAAATTCTTCCTTGTGATACTAATGAGCGCCACGGGCCTGGTCTTCCTCACCGGCATC TTCCTGGGACGGGCCACCAAACGGGCAATGGGCTTAAAGAATAAATAATTGAAATATTAGACTGTAGTGGCACTGTGTAG GCGGAGTGTGCATGTTTGTGAGCTTGCTGATTTCCATTTCGGCCCATCCATGACCCCATTCCGAAACCCAGAAGTATACT TTCACTCAACCCTGTCTGCCTTTCGATATTTGTGTGAGCAATTTGTGGCTTAGTCGTTTGTAAGACCAAAAAAATACGCG AGAAAGGAGAACACAATCAAATATACTAGTTCCATTCTTAGGGCTCTTTTCGTTATAGCTTTTTTGTAACAATTTCACTT GTATATTAAATTGTATTTTTGTAAACAAAAGCCCCTTCTTTTTAACTATATCCACATGGAAGGCATCAACAGATGTTTCT CTTTGGATCAATACCAGAGCGCTAATACGGCAGAGCGCCTAGTTTTGAGTACAAAAGTTAAGTAAAACTTGGGATAGGCT TATCAATTTAATCAAATAGCCAAGGTAACTCAAAGTATAAAATTTAATATTATGTCCTTGTGGGCTTATGAGAATTAAAA TTTGTGTTAATTGATTAAGCTTGACTCTAATTTGTACCCTAGACACGCCTTTGTTATTATTGATATTAGTAATTCTATTA TTATCCTTTGTTTTACGTTAAAAATTACAATTTCAACGAACTGTAGCTCTACAGGTGTTTTGGTATATTTGCACAAAAAA AAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.