Comment: 0-22 hr AEL
CTCGAATCTGCAACAACGTGCACGCAAAACTCTCAAAATTTTCTCAAAAACATCAAAATCACAAGGCGAGCGGAAAACTC AAGTATAAACGCTAAACGTGTCGCAGTTTCTTGGAAAATATCTAATATTTTTGGCAACATCTCGTGTTTTGTGCAATTGT GTGTGTGTGTGGAGCAGAAGAGCGAACAACCAGGAAAAATACCAACTAAAAAAAAACTCTGTTCTGAGAATGTGAAAACA TTTTTAACATCGAAAAACTCCGAAAGCTACAACATTTAAACAAATCAAGGGAACAACAGTGGACAACTGAATGCGAAATA CCCTTTAAAGTGTACCAGCAACTAAATTGGCCACTTAGCACCATCGATATTGTGCCCCACCTGCGGCATTTTCATTATTT CGTGGCAACCAGGTGATCCGTAGGCCTTAAACTGGCGCTCAAGGTCAGGAAGCATCTATCCTTCTACTCGCCGACGCTGC TGAGCGCCGCCTTCACCAGTCTGAACGGTGTGTACAACAACAGCAGCAATAGCAGCGATCCCGCTCCGGACGAGGAGCCC GACCAGCCAGCGAACCAGCACCACCACAGCCACCATTCAAGGGTGAGCCACCACCACATTCCGCGGGAGGCCAAGCAGGA CAGGGATCGCGAAACGGAGCGTGACCAGGAGATGGGCGGCCCAACGGCGCCGGTAGCCAGCAGTGGCGAGGTGGGACAGA CGTACTGCCTCCGCTGGAACAACCACCAGACCAATCTGGTACAGATTCTACACGCCCTGCACGAGGTGGGCAGCTATGTG GACTGCAGCCTGGTGGTGGACGATGAACAGTTCCAGGCCCATCGCGTGGTTCTGGCGGCCAATTCACCGTATTTCCAGCA CATCCTGAAGGATGTGCCGCAGGACCACTGCAGCATCATACTGCCGGGAGTGAAGGGCTTCGAGATTGCCGCTTTGCTGC AGTACATGTACACGGGCGAGACGACGGTGACCAAGAGCCAGGAGCCGGAGATTCTGCGCACAGCCAAGGAGCTTCAGGTC AAGGGTCTGTACGACAACTTGATGAAGTTCAATCATGCCAGCGTCACGCCCACCTCCAGTTCGGGAGCTGGCGGAGCCAA GCCCCAGAACGGATCAGCATCCAACCACTCCTCATCCGTGATCTCCACATCCACGCACATCTCGCCCAGTGCCGCCATCT CGAGCAGCTGCTCGCCACCACCGCCGCCGCAGTTTGGCTATCAGCCGGGCTACAGCCATTATCCTCAGCAGCAGCCGATG TCGGCTAGTCAGATTCCGGCCGGTGAGGCTCCACTGACCCCCACCCAGGCCACACCCCACTCGGCGGCCTCGGGAGCGGG AGAAGCCGGTGGACAGTGGCCACTGACACCCTCCGCCGCCGCCGCCATGTTGAACTCTGTCTACGAATCAGCCGCCGATA TGAATCCTCTCAAGCGCAAGAAGCTGTCGGCCATTTCAAGCATGCTTCTGAGTGGAAACCGCGACACACCCATCCTGAGA AACGTTCTGGCCCAAGCTAATCCCGCTGACTCCTCGCAACCCGGACCAATGAATGCCAATGGAGAGAAGACGCCCACTCA TCCCCACCAGAACACCCAGCTTCCTGCCGGATTGGGAGTAAGTGGAGGCGAGCGCAACCACAGCTTCAATGGCTCCGACT ACGGCGGCGACAAGGAACCACTTTCGCCCTACACAGATCGTTCCTTTGAAGAGGAGACCGGTCAGTCTGGCGGCAAGAAG CCGGAATGGAAGCGATACAAGCAGTATACCAGAGCGGATATGATGTGCGCCATCCAAGCGGTGAGGGAGGGAATGAGTGC CCTGCAGGCGAGCAGGAAGTACGGCCTGCCCAGTCGCACTCTCTACGACAAAGTCCGTAAGCTGAACATCACCACAGGAC GTGGCACACACCGCACACCCAAGCGCAGCCCGCCGGGAGCTGAGTCCTCACAAGGTTTCTCCTATTCCGCTGCGGCAGCC GCTGCTGCACACAACTACGGTCATGGACATGGCCATCACTCGGAGGTGAAGCGTGATCAGAAGGTGGACCATGTGCCCGC CCATCACGGCATGCCGCCCACCATTCCACCCTCAGCTGCGGCCCTTCTGGATCACGCTTTCCTTCAGCAGGCTCTGGAGA ACCGTGGTGGTGACATGGCCGGCCGAGAGGCTCTCCATGCCATGGCTCTGGCCGCAGCGGCCCATGCCGCTGCCAATCGC CTTTCCAGCAGTCCAGCGGAGATGGACCAGCGCTCCAATGGCCATGGCATGCGGTCGCCAAGTCCGCAAGGTCGTCACTA CCCGCACGAGCAGGAGGCCATGGACTTGGAGATGGAGAAGCACGAGCAAAATATTATCAAGAGGGAACGCGACCAGGACG AACGTGAAGATGCGGACGATGAGGAGGATCATGAGCAAGAGCACGTGGAGGATCTCTCGCTGGCTAGAAAGGAGCGGCCA CCATCGCCCTACTCGCCCCAGCCAACCGAGGCTGGTGCCGGCGTGATTATGCACGCCAGCAGTGCGTCCGCCAATGGCAA GGATCACGTGGACTACCCATCCTCGCCGCAGTTCGTTCCCATTGGTCTGAAGAGGGAGCTGATGGAAGGCGAGGACGCTC AGGCCCGGGCCGACTGATTAACGCTCACCATGGCCTGGCAGTATGCCACGGACAGCCTAGACTCCTTGGAGTAGGCCAGG CTTAGATAGCAACTCCTATTATTCAAACCCAATTAGATCCATCCTTGTGTTACTTTCCTTACTTACCATCGGCGACTCAG GCGCCGACCAGCGTTGGTGAAGCTTTAAACGAAGAAACCTCATCCCAGTCGTTGTGGGAATCATCCGCACCAGCACCTGG CACCCATCGAAAATCAAACCCACACACTAAACTAAATTGTTGTCAGCATCACTTTGTGCCTAAAAAAAACAGAAATGTTA GGATATAGATAGTAGGATAGGACAGCATAGAATACAGATAGGTACCCTTAGGCTAGGCACTCGTAGGCCATCTTTGGAAA CCAGCTAGTTGTAGGCCTTAAAACAGTGTTACTCCACCATCCCAGATGCCGGCCAACTGACAAAACTAAGATATGTGTGC ATATGTAAACCCACTTACATAAGACTGAAGGCGGAATCGGGAGGCGGTGGAGGAGCTATCTAAGGACAGAGCAGTAGAGG CTCCCATCAGAATGGGTTAGCGGGTTAGCTACACCTTGTACAACTTTGTTAAATGCCAGTGATTTGTTTTAAAAATTATT AGCACTAGATTATTTATAACGACCATGCATGTACGTGGCCTTACTTTTATGATTTTCGAATCGAAGAGAAACCAGAAGAT CCCACTTGAACAGAACTACCTCAAATAGCGTATCTTTGTTAGCCTCATGTTATCCTGTTAGCGAGATATTTAAATCGTCC TGGGTTGAATTTATAAGACTGACACCGACTCAAACGTACCACGAGCAAGAGCACACGCATTAAAATAAAATAAAATAAAA TTATAAACTAAACTTAAATACGAATCGAGATCCTTAGTTGAAAATCTGTAAGTTTCCCGCAAATTGATTAGTTCTAAGGC CATCAACTAAACGTAAGCACTAAAACTAAACTATTTATAAGACAAAACAAAACAAAACAAAAAACAACACACATGAAAAC ATGCGCTGGGGGCAATGCAATAGGCTCTTTAATTATAAACACCTACACTTATGCAACTTATATAGTCGTACGGTCGATAA TGTTAAATTGTTATACGAGTAACTCTGTACATAGCATCATAGTCCACACATACACATGCGAATTATAAATCTAGTTTTAA GTATTCCGAATTGTACTGCAATTTAAATTATCCCTAGACACCCGACTTTCATCATGTAAAGATCAGCGAAGGAACGCGAA ATAAACTGACTGAAGAATCAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
