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FB2026_03
,
released September 17, 2026
Comment: 0-22 hr AEL
TCTTTGAGCGCAAGCTGGTGTCACACATGTCCAAGGGATTCGATCCGTTTAAGCCGCTGTCGATCAACACCAGTGCCCTG GAGTCGTCTGCCAGCACGTCGAGCACTTCGGCCACCACGACGGTGAAGAAGCAAAGTGTGCTGTATGAATCCCTGCAGCA GCATCCATTGGCCAAGTACCATCAGACGCAGCAGCAAACGACAACGACGACGACGGTGGATGGACAGACGAGTAGCCGGA TGGAGCTGCACAAGGCGACCCTGGCGAATGCCACCATCGATCCGGTGCCAGTTGGATCCAGCACAGCAGCCATTGTGGAC AGTGCGTCGGGAAGCGGCAACGTTGTGCGGCGCTACAAGCCATTATCCTCCAGCTCCTTTGACGAAGGCAAACCAATGCG TAGGATATCCTACCTGCGGGCAACCAACAATGAGACCGACTTCAATGCGGAGGCTGCCGCTGGCGGAGATGCCGCGGCAA CAGGGGCGTCAGGACCAGCATCTGCATCAGCAGCTTCATCTGCACCTGGACCCACGCCCTCATCTGCTCCTGCACCAGCT CCAGCACCAGCACCCCTGCCCATTTCGATCCCCATTCCGGTGGCTGCTGTCGTCGAGCCGCAAAAGACGAAGTCCCTGGT GGAGGAGCATCCGGATCCCACCTACGATGTGATCGGAGGCACAGGTGGTGGCCACGAGTTCATCGAGACGCCGAACGGCC TGGAGGATGTGCGTCTGTCGGCCCACGGGAGTCGGAGGGCCTCCATGAGCAGTGACATGCGAAGCAGCATCCACATTGAT GCCGAGTTAAATGGCAAGTATGTGCCAAACGAACTGGAGGCCTTTGAGACGGAAATCGAGATCAAGTCCTCTTGCATCAA CGGAAAACGCATGTCCGAGTACCGATCATGGCGTCAGGTGAAGCTGGAGATCAAGGGAGATCTGCTGCGTATCTACTCAG GCCGACATGCCAAATCGGAGCACAATGTGATAGAACTTGATATTAGAAACTTTAAGTTCTTCGACGAGTCGATGGATAAG AAGAAATACTTGATCCGTATGCAGTCGAAGCCATCGCCCAGTGGAGCCCATTTGGCCTCCTTGGGCAACGAGTTAAACCT GGACGAGGGTCACGACAAGCTGCTAACCTCGTCGGGCAGCAGCAGTGCGAATGAGCCCGCTGCCAACGCAGCATCGGCCC TGACCGCATCCACCTCCTCGTCCACCTGCAGCAGCGGTCCTTACACGGAGATCCTATTCAAGACGAAGAGCAGCAACGAG ATGAAGCGTCTGTTTGGACTTCTGCAGTGGAAGGATAGCCTCAACTACGATGATAATGAGCACCAGCCACAGGGCAAGCA GTTGGCAGAACCACAGAAAACAGTGGCCAGCGCCAGTTATCTGGACGACGTCCAAGGTGGAGCGAGTGCAGCCGACAGCT CACCCTTGAGCTCCCATTCCGGTGGCATTGCAGACGCTAGTGGCAGTCATCATGTTTCTGCGCTACCAGCAGCTGCGGCT ATTGTCGCCGCCACCAGCGACTCCATTTCGCCGGTGATGAAGGCAAGGAAGTCCTCTTCGCACAAGCATATACCTGATAA GGATTTGGGATCGCCCAAATCGAAGAACTGGAAGGATTTGCTCTTCCGCCGTGGCGGCAGTGGCAGCGGAGGAGTGTCAC ACCACGACTTGGCTTCGCCATCCGCCTGTGCAGCCAAGCAGATTGGATCTATTGGCGTTCCACTGCGCAGCTGTCCCATG TCGAAGGTCAATGCATATGTGCCGCATCTGGTAGAGGTGTGCACGAACATTGTGGAGACAAAGGGTCTGGGAGTGGTGGG CATCTATCGCATACCCGGCAACAAGGCGGCCATATCAGAGCTGTCCGAACTTGTTAACACCAAGGACTTTCAGTTCGAGA GCTGTGCCAGCGATGACCGCTGGGAGGATGTGAATGTGGTGAGCAGCCTGCTCAAGTTGTTTATACGCAGCTTGCCCGAT GCCTTAATGCCGGCAAGTTACTATATCAACTTTATTGAGGCGGACAAGAAGTTTGGCCTGGAGAGAATTGTACTGTTGCG CGAGATTGTGGAATCGTTACCACGGCATCCCTACGAAACGATGAAGCACTTGATTCGGCATCTGTGCCGAGTTAGCGGCA ATTGCGATGTGAACCGCATGGAGCCCAAGAACCTGGCCATCATATTTGGGCCATCTATAATCAGGACGCCGAACGATACT CTGGAAACGGCAGTCAAGGACATGAAGCATCAATGCCGCATCGTTGAGCTGCTGGTCACACAGTACGACTACTTCTTCGA AGGCGGCAGTCTGCCGGATATAGCCGAAGTAAGCGGCGGTACAGCTGCCGTAAGCGCCGCTCAGCCGCAGCAGCAGACGC AGGAGGATCAGACGAGCATGCTGCTGCACAACCTGTCGAAGATCGAGCGGATCACAGAGCGCGAAACCACGCGCACCTCG CGCTTTATGCCGCAGCTCAGACGAAAGACCCATGGCAAGCGCAGCGCCGTAAACTCGGACACGTACTCCGGCGAGAGCGT TCTGGTAAGCGGGAGCTCCTCCAACACCACCACCACCACCACCACCACCACCCACACCACAAGTAGCACAAGTAGTAGCA ACACCATCACCACAACCATCAGCACCACCACCATCCAGCAGCGACGGGCGCAGCGCAAGGCTGTGCAGAGCATTTGCGAC CAGGCTCTAATCCTGCTCCTGCCCACACCCGTTTCTTCCGTCTCTCTTCCCTTTCAGTCGTTGGACTACGCCAAGGCGTC CGCCTCTGCCTCGGCATCCGCAAGCAGCACCGCCAGTACTTCGAGCAGCTCGACGCTTCACAGCGGGGGCAAGGCATCCG CCTCATCCTCATCCAGTTCCACTACCAAGTTCAGCTCATCGTCATCGTCGGCGTGCTCAACAACGGCGGTGGCTATTTCT TCCTCCATCTCGGCGACGTCCTCCAAATTGTTAAGCGCCAATTTTAAGAAGCGTAGCACAGGAGGTTTCTTGGGCTCAAG ATCCTCGCACCAGACGCGCAAGGCCAGCTTGGACGAAAAGGACTCAGGACAGTCGTCTGGCAGCCTTGGTCACAGCCTGG GAGTAGGCATAAATCTGGCCAAGGAACAACAGCGCAGCAGCGTGGACATATCCATTCTGCTAACGGACGACGACTCTAGC AGCAGGCTAAGCGATACGGGTTCCATGTCGCTGACGACCATTACGGATACACTGGACAGCAAGCTACGCAACTTGCGCAG CGGCTCCGAATCGAATGATGAGAATGGATCGCCCGAGTTTCCCAAGAACCGGCGGCACACCTTGGGCCAGCCTCTGCACA TGCACTCCGAGAACATTCCGTATGCGGACGAGTCGCCCGAGCGGCTGTTCCATCAGTTTTGCAATCCCCTGGAGACAGCC CCCTTATCCCTGCATCTAAGCCGCTCCTGCACGGCCACAAATACAATTGGTGGCGATGAAAAGCCTTCGAAACAGTCGGG TGGAGTTGCACCACTGCCCCCAAGTTTGCTTAAGGCCGCACATAAGCTACAGCACTCAGCCACGGTGCCCATTCATCAGG GAAGCTCCACTGCACCAACTAGCGGAGCAGCCACGCCAGTAGGCACGCCAACCGGAAGCGGTGGGGCCGGAGCTATTCCC TCGCGCAGCTCATCGAGCAGTGTCACGACGCTAACCAAAAGCATACAACCCAGGTTCAGTAACTATCTAAGCTACGAAAG AGGCAATGCAGCCGCTGGAGGCGCCGAAAGGGTGGTGGGATCCGCCGGAGGAGCTGCCTCCGACGACGATTCCGAGGGCT CGACCACCAGTGATCCCAAGGAGAAGCTGCTTCTCGGTGAACGACCGTCGCCTTCGTTTTTTATCGAGCGCTACAACAAA AAGAGACGCGATCATCGGTTATTTCGCAGCGCCAGTTTCAATTGCCGGAACTACTCCACCAGGCACATAACGGCGCAGAC ATCCGGAGGAGTAGCGGCGGGAGTATCGGGCGGAGTAGCTGGAGCGGTGGCGGTGGGGGTGGCTTGCTGCCAGGCATTGA GCACCACTGGCTTGACCAAGGACGAGAAGACTGATATGAACCTGACCAAGAAGCGCCAGATCCAGAACAAGCAGAATCGG TCCATTAAGCGGCGGCACACAGTAGGCGGACCTCACGACTATTCCGCCAGCAATGGTGGCTGCTCGAATCACTCACACGG TCACGACCTGGCAGCCATCAACTACAATCACCACAATCGCCATCACCAGCAACAGTTGTTGAAACTGGGCAGCGCTACAT CTGGCGGTGGAGGCAGTGGCGCAGCCGCGGCCGAGACGACGACCACCACAACGACCACCACCACGGTGCTGCGGCGCCTC GGTACAGGTCAAGCTGGCGCCGAAGCTAGTGTGGCATTGCCCGCATCAAGTTCCGGATCGAGCAGCAACAACAATAACTC GCCCCAAAGCGATGGCAGTAATGGCAACGGCGGTGGAGTGGCGGGAGTTGCCTCCAATGCACCACCAACTGGCGTAGGTG TCGGTGGCAACAACAGCAGCGGAAGCAGCAGCAACAGCAGCAGCACCAGCACAACTCCCGCTGGGAACGGCGATCAGGTC CTGGAAGTGGGCATACGTATCAAGAACATTAGCCGGGGTCGCTTCTAAAAACCTGTATACCCAGACTAAGAAAACAGACG GTGGCAATTTCACATGGTGAAACACACATGAAACACTCTGGGCGAACTCACATTCTTAGCAAACAGTCTATACAAAGCTA AAAGTGCTAATATGGCTAAAAGCAGGCAGTCTGCTTATATCGCGTTTTAAAGAACCTGACAAAAAACATAGTTAAAAATA GCTCCTAGTGCAGATTGCTGCGACAAGACCAACCCGAAATAGCAGTTGCTTAAATGTGGTTAACCTGATTAATTGACACT TTGAAACCCACAGTTTACAAGAGTATTAACGAAAGGTTTAATATATAACCAAAAGGCATAATGAGAAGATATTTAACAAT AGTCTGTTAAACACACAAAAGCTAAACACACCTTTAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.